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Advances in the development and application of electron microscopic techniques have occurred recently such that the electron microscope has evolved to become an essential tool in both basic and clinical research. Use of this instrument has contributed significantly to the formation of new perspectives and concepts concerning cell fine structure. These structural perspectives are now being integrated with specific functional, biochemical and pathophysiological events and processes of cells and tissues. Most recently, utilization of innovative electron microscopic techniques such as freeze-fracture, freeze etching, and scanning and high-voltage electron microscopy offers both the basic and clinical scientist potentially fundamental insights into many morphodynamic processes related to the activities of cells and tissues. Such an approach has been especially rewarding when applied to the dynamic events of gametogenesis and early embryonic development. The chapters comprising this book have been selected and edited with the aim of providing an up-to-date and comprehensive account of the most important aspects of vertebrate gamets and embryos as revealed by the integration of several different submicroscopic methods. The organization of the chapters is designed to indicate present gaps in our knowledge of the developmental and reproductive biology of gametes and the developing embryo and possible Iines of research which may lead to a lessening of these gaps.
The Reproductive Biology of Bats presents the first comprehensive, in-depth review of the current knowledge and supporting literature concerning the behavior, anatomy, physiology and reproductive strategies of bats. These mammals, which occur world-wide and comprise a vast assemblage of species, have evolved unique and successful reproductive strategies through varied anatomical and physiological specialization. These are accompanied by individual and/or group behavioral interactions, usually in response to environmental mechanisms essential to their reproductive success. - Is the first book devoted to the reproductive biology of bats - Contains in-depth reviews of the literature concerned with bat reproduction - Contributors are widely recognized specialists - Provides a powerful database for future research
This book will give an overview of insect ovaries, showing the diversities and the common traits in egg growth processes. The idea to write this book developed while looking at the flood of information which appeared in the early 1980s on early pattern formation in Drosophila embryos. At this time a significant breakthrough was made in studies of this little fly, combining molecular biological methods with classical and molecular genetics. The answers to questions about early pattern formation raised new questions about the architecture of ovaries and the growth of eggs within these ovaries. However, by concentrating only on Drosophila it is not possible to form an adequate picture of what is going on in insect ovaries, since the enormous diversity found among insects is not considered sufficiently. Almost forgotten, but the first to study the architecture of ovaries, was Alexander Brandt writing in 1878 in aber das Ei und seine Bildungsstaette (On the egg and its organ of development). More than 100 years later, a series of ten books or more would be required to survey all the serious informa tion we have today on insect oogenesis. Thus, this book is a personal selection and personal view on the theme, and the authors must be excused by all those scientists whose papers could not be included. The book briefly describes the ectodemes, i. e.
This ground-breaking atlas of oocyte ultrastructure forms a practical guide to assisted reproduction. Extensively illustrated with transmission electron microscopic (TEM) images, it contains chapters on oocyte maturation, fertilization, fertilization failure, pitfalls and technical problems of intracytoplasmic sperm injection, and failed fertilization in vitro to explain the principles and evaluation of TEM images. A large chapter of TEM images of the ultrastructure of human oocytes illustrates fertilization failures after ICSI and IVF and the ultrastructure of germinal vesicle oocytes and metaphase I oocytes. It also contains detailed information about abnormal metaphase II oocytes with sections on autolysis, multi-vacuolated mature oocyte, refractile body, and fragmented oocyte. The concluding chapter describes materials and methods for single-cell transmission electron microscopy of the human oocyte.
Personnel working in assisted reproductive technology often lack the opportunities for dedicated training in the specialized techniques and technologies required for the procedures. As such, success in the form of live birth rates can range from over 50% to less than 10% per treatment cycle. This comprehensive introductory textbook is an essential resource for trainee embryologists, medical students and nurses. The recent revolutions in biotechnology and molecular biology involved in delivering assisted reproductive services are thoroughly discussed. Basic knowledge such as the development and physiology of both male and female reproductive systems is covered, with practical aspects of IVF including gamete and embryo manipulation, cryopreservation and genetic testing explained in detail. A full description of the optimal structure and management of the IVF laboratory is given, helping ensure procedures are safe and effective. Extensive and highly detailed colour illustrations bring the content to life and aids readers in their understanding.
This volume describes the myriad ways in which fish have approached problems of reproduction — it is an amply illustrated comparative study of the microscopic structure of the female genital systems of fish. The timing of its appearance is auspicious in that it coincides with the decline of the golden age of descriptive morphology. It is a compilation of thousands of micrographs from classic works in the field. The volume should prove valuable to investigators studying fish in areas such as ecology, physiology, and reproductive biology who may view histology as essential in their work but have little background in this area.