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This symposium is the third in a series featuring the propaga tion of higher plants through tissue culture. The first of these symposia, entitled "A Bridge Between Research and Application," was held at the University in 1978 and was published by the Technical Information Center, Department of Energy. The second symposium, on "Emerging Technologies and Strategies," was held in 1980 and pub lished as a special issue of Environmental and Experimental Botany. One of the aims of these symposia was to examine the current state of-the-art in tissue culture technology and to relate this state of technology to practical, applied, and commercial interests. Thus, the third of this series on development and variation focused on embryogenesis in culture: how to recognize it, factors which affect embryogenesis, use of embryogenic systems, etc.; and variability from culture. A special session on woody species again emphasized somatic embryogenesis as a means of rapid propagation. This volume emphasizes tissue culture of forest trees. All of these areas, we feel, are breakthrough areas in which significant progress is expected in the next few years.
2. IMPORTANCE OF NITROGEN METABOLISM 2. 1. Range of naturally occurring nitrogenous components in forest trees 2. 2. Gene expression and mapping 2. 3. Metabolic changes in organized and unorganized systems 2. 4. Nitrogen and nutrition 2. 5. Aspects of intermediary nitrogen metabolism 3. NITROGEN METABOLISM IN GROWTH AND DEVELOPMENT 3. 1. Precultural factors 3. 2. Callus formation 3. 3. Cell suspensions 3. 3. 1. Conifers 3. 3. 2. Acer 3. 4. Morphogenesis 3. 4. 1. Nitrogen metabolism of natural embryos 3. 4. 2. Somatic embryogenesis 3. 4. 2. 1. Sweetgum (Liquidambar styraciflua) 3. 4. 2. 2. Douglar-fir and loblolly pine 3. 4. 3. Organogenesis 4. OUTLOOK 11. CARBOHYDRATE UTILIZATION AND METABOLISM - T. A. Thorpe 325 1. INTRODUCTION 2. NUTRITIONAL ASPECTS 3. CARBOHYDRATE UPTAKE 4. CARBOHYDRATE METABOLISM 4. 1. Sucrose degradation 4. 2. Metabolism of other carbon sources 4. 3. Hexose mobilization and metabolism 4. 3. 1. Cell cycle studies 4. 3. 2. Growth studies 4. 3. 3. Organized development 4. 4. Cell wall biogenesis 4. 4. 1. Primary cell walls 4. 4. 2. Cell wall turnover 4. 4. 3. Secondary cell walls 4. 5. Carbon skeleton utilization 5. OSMOTIC ROLE 6. CONCLUDING THOUGHTS 369 12. THE USE OF IN VITRO TECHNIQUES FOR GENETIC MODIFICATIO~FOREST TREES - E. G. Kirby 1. INTRODUCTION 2. IN VITRO SELECTION 2. 1. Natural variation 2. 2. Induction of variation 2. 3. Selection techniques 2. 4. Plant regeneration 2 . • 5. Applications x 3. SOMATIC HYBRIDIZATION 3. 1.
Thirty-four Populus biotechnology chapters, written by 85 authors, are comprised in 5 sections: 1) in vitro culture (micropropagation, somatic embryogenesis, protoplasts, somaclonal variation, and germplasm preservation); 2) transformation and foreign gene expression; 3) molecular biology (molecular/genetic characterization); 4) biotic and abiotic resistance (disease, insect, and pollution); and 5) biotechnological applications (wood properties, flowering, phytoremediation, breeding, commercialization, economics, and bioethics).
Since the first edition of our book "Tissue Culture in Fores try" in 1982 we have witnessed remarkable advances in cell and tissue culture technologies with woody perennials. In addition to forest biologists in government, industry, and universities, we now have molecular biologists, genetic engineers, and biochemists using cell and tissue cultures of woody species routinely. There fore, the time has come for an update of the earlier edition. In our present effort to cover new developments we have expanded to three volumes: 1. General principles and Biotechnology 2. Specific Principles and Methods: Growth and Development 3. Case Histories: Gymnosperms, Angiosperms and Palms The scientific barriers to progress in tree improvement are not so much lack of foreign gene expression in plants but our current inabili ty to regenerate plants in true-to-type fashion on a mas sive and economic scale. To achieve this in the form of an appro pr iate biotechnology, cell and tissue culture will increasing ly require a better understanding of basic principles in chemistry and physics that determine structural and functional relationships among molecules and macromolecules (proteins, RNA, DNA) within cells and tissues. These principles and their relationship with the culture medium and its physical environment, principles of clonal propagation, and genetic variation and ultrastructure are discussed in volume one.
Presented here is another classic from this series and deals with general aspects of micropropagation of plants for commercial exploitation. It includes chapters on setting up a commercial laboratory, meristem culture, somatic embryogenesis, factors affecting micropropagation, disposable vessels, vitrification, acclimatization, induction of rooting, artificial substrates, cryopreservation and artificial seed. Special emphasis is given on modern approaches and developing technologies such as automation and bioreactors, robots in transplanting, artificial intelligence, information management and computerized greenhouses for en masse commercial production of plants.
This book presents latest work in the field of plant biotechnology regarding high-efficiency micropropagation for commercial exploitation at low labor and equipment costs. The book consists of 18 chapters on establishing advanced culture systems, techniques as well as latest modification protocols on a variety of crops. It also discusses new methods such as nylon film culture system, light-emitting diode and wireless light-emitting diode system, stem elongation, wounding manipulation and shoot tip removal, in vitro hydroponic and microponic culture system, thin cell layer culture system etc. Plant cell tissue has been developed more than fifty years ago. Since then applications of in vitro plant propagation expanded rapidly all around the world and played as an important role in agricultural and horticultural systems. This book will be of interest to teachers, researchers, scientists, capacity builders and policymakers. Also the book serves as additional reading material for undergraduate and graduate students of agriculture, forestry, ecology, soil science, and environmental sciences.
Modern Applications of Plant Biotechnology in Pharmaceutical Sciences explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how these methods can lead to more effective, safe, and affordable drugs. The book covers modern approaches in a practical, step-by-step manner, and includes illustrations, examples, and case studies to enhance understanding. Key topics include plant-made pharmaceuticals, classical and non-classical techniques for secondary metabolite production in plant cell culture and their relevance to pharmaceutical science, edible vaccines, novel delivery systems for plant-based products, international industry regulatory guidelines, and more. Readers will find the book to be a comprehensive and valuable resource for the study of modern plant biotechnology approaches and their pharmaceutical applications. - Builds upon the basic concepts of cell and plant tissue culture and recombinant DNA technology to better illustrate the modern and potential applications of plant biotechnology to the pharmaceutical sciences - Provides detailed yet practical coverage of complex techniques, such as micropropogation, gene transfer, and biosynthesis - Examines critical issues of international importance and offers real-life examples and potential solutions
Food security, crop protection, biodiversity, and human and environmental health are among the main needs and concerns of society. Modern biotechnology and life sciences represent a constantly evolving area that is key for the rational use of natural resources – resources that in turn are indispensable for societal development. This book features the outcomes of the IV International Biotechnology and Biodiversity Congress, held in Guayaquil, Ecuador, 2018. It includes extensive reviews of the trends in agricultural and forestry biotechnology, molecules and materials biodiscovery, ethnomedicine, environmental impact and bioindustry research, describing many of these topics from the Latin America perspective and showing how the biodiversity and ancient knowledge of these countries are vital for worldwide sustainable development.
Conference on Tissue Culture as a Plant Production System for Horticultural Crops, Beltsville, MD, October 20-23, 1985
Plant Cell and Tissue Culture gives an exhaustive account of plant cell culture and genetic transformation, including detailed chapters on all major field and plantation crops. Part A presents a comprehensive coverage of all necessary laboratory techniques for the initiation, nutrition, maintenance and storage of plant cell and tissue cultures, including discussions on these topics, as well as on morphogenesis and regeneration, meristem and shoot tip culture, plant protoplasts, mutant cell lines, variation in tissue cultures, isogenic lines, fertilization control, cryopreservation, transformation, and the production of secondary metabolites. Part B then proceeds into detail on the specific in vitro culture of specific crops, including cereals, legumes, vegetables, potatoes, other roots and tubers, oilseeds, temperate fruits, tropical fruits, plantation crops, forest trees and ornamentals. Plant Cell and Tissue Culture is, and is likely to remain, the laboratory manual of choice, as well as a source of inspiration and a guide to all workers in the field.