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The Chlamydomonas Sourcebook, 3rd Edition Cell Motility and Behavior (Volume 3) The gold-standard reference?introducing this multidisciplinary science,?fully revised and updated with the latest discoveries Originally published as the standalone Chlamydomonas Sourcebook, then expanded as the third volume in a three-part comprehensive gold-standard reference,?The Chlamydomonas Sourcebook: Cell Motility and Behavior has been fully revised and updated to include the wealth of new resources for the Chlamydomonas community. Reflecting the significant advancement in?the understanding of the role of basal bodies and cilia play in human diseases, this volume employs quantitative proteomics and mass spectroscopy as well as?cryo?EM tomography and single particle?cryo?EM. Other topics such as current insights?on?mitosis and cytokinesis, ciliary assembly and motility, intraflagellar?transport, and more help build an understanding of human diseases of the cilium. Cell Motility and Behavior presents the latest in research and best practices, making this a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, and biochemistry; crop scientists; plant physiologists; and plant, molecular, and human disease biologists. Provides an essential reference to a model species for the study of mechanisms of motility in free living cells? Includes methods for Chlamydomonas motility research? Includes a table listing the known proteins (with NCBI accession numbers) for each structure discussed, and the known mutations that affect each structure and process
This volume reviews virtually everything that is known about cell motility and behavior in Chlamydomonas. World experts in each area focus on mitosis and cytokinesis; flagellar assembly and motility; intraflagellar transport, and more will use this reference as a guide for understanding human diseases of the cilium. The volume is richly illustrated and is supplemented by a Website containing both classic and previously unpublished videos of cell motility in Chlamydomonas. Because Chlamydomonas has been the premier model for investigating the function and behavior of cilia and flagella, the chapters summarize the current state of knowledge in these areas as it applies to all ciliated organisms. Thus, this volume will be an essential source for all students and researchers interested in cell motility. This volume is part of a 3-Volume Set (ISBN: 978-0-12-370873-1) and is also sold individually. - Includes a table listing the known proteins (with NCBI accession numbers) for each structure discussed, and the known mutations that affect each structure and process - Essential reference to a model species for the study of mechanisms of motility in free living cells - Includes methods for Chlamydomonas motility research - Companion Website with videos illustrates the marvels of the Chlamydomonas flagella in action
Dr. Harris has played a major role in the development of this organism as a model system. Her previous version of the Chlamydomonas Sourcebook which published in 1989, has been a classic in the field and is considered required reading for anyone working with this organism. This latest edition has been expanded to include three volumes providing molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism. Methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media, are included. Additions to this volume also include help with common laboratory problems such as contamination, student demonstrations, and properties of particular strains and mutants. This volume is part of a 3-Volume Set (ISBN: 978-0-12-370873-1) and is also sold individually. - Expanded revision of gold standard reference - Includes latest advances in research, including completion of the genome - Provides broad perspective with studies in cell and molecular biology, genetics, plant physiology and related fields - Available as part of a 3-Volume Set or sold individually
Dyneins: The Biology of Dynein Motors, Second Edition, offers a broad view of dyneins from structure, composition and organization, to biology of dynein function in both cytoplasm and cilia. As the second book in a pair on this topic, these works provide an overview of dyneins, from structure and function, to dysfunction and disease. Since the first edition, enormous strides have been taken in understanding dynein structure, its organization in the axoneme, single molecule motor mechanics and the consequences of defects for human biology, disease and development. This second edition is extensively revised, with coverage expanded from 24 to 42 chapters. Much of the expanded coverage occurs in Volume Two on dynein dysfunction and disease, such as the role of dynein and cancer, while Volume One covers the history and evolution of dyneins, dyneins in ciliary biology and cytoplasmic dynein biology. - Presents a broad-based, up-to date view of the biology of dynein motors - Discusses approaches from genetics, molecular biology, biochemistry and biophysics - Includes a companion website with movies of dynamic cell behavior - Covers the topic in comprehensive chapters written by world experts
Along with its companion volume on axonemal dynein-mediated motility, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella. - Covers protocols for cilia and flagella across systems and species - Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time - Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures
This book draws together recent data on both cytoplasmic and flagellar dyneins and the proteins they interact with, to give readers a clear picture of what is currently known about the structure and mechanics of these remarkable macro-molecular machines. Each chapter is written by active researchers, with a focus on currently used biophysical, biochemical, and cell biological methods. This is a useful handbook for frontline researchers as well as a textbook for advanced students. Besides comprehensive cover of structural information gained by electron microscopy, electron cryo-tomography, X-ray crystallography, and nuclear magnetic resonance, this book provides detailed descriptions of mechanistic experiments by single-molecule nanometry. The reports include in vitro studies of the motility of reconstituted complexes and in vivo studies of organisms expressing mutant dyneins. The recent technical improvements described, which have played an important part in recent advances, include the expression and preparation of recombinant dynein heavy chains or individual subdomains.
The Chlamydomonas Sourcebook, 3rd Edition Introduction to Chlamydomonas and Its Laboratory Use (Volume 1) The gold-standard reference?covering the basic biology of the Chlamydomonas alga and techniques for its laboratory analysis Originally published as the standalone Chlamydomonas Sourcebook, then expanded as the first volume in a three-part comprehensive gold-standard reference,?The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use?has been fully revised and updated to include a?wealth of new resources for the Chlamydomonas community. Early chapters cover current understandings of its taxonomy, ultrastructure, cell and life cycles, and nuclear and organelle genomes, followed by technique-oriented chapters covering such topics as cell culture, mutagenesis, genetic analysis, construction of mutant libraries, and protein localization using immunofluorescence. This volume presents the latest in research and best practices, making it a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, and biochemistry; crop scientists; plant physiologists; and plant, molecular, and human disease biologists. - Remains the only complete reference to provide both the historical background and the most up-to-date information and applications on Chlamydomonas - Includes best practices for applications in research, including methods for culture, genetic analysis, genomic and transcriptomic analysis, and mutant screening - Helps researchers solve common laboratory problems, provides details on the properties of particular strains, and offers a comprehensive survey of molecular approaches - Provides a broad perspective for studies in cell and molecular biology, genetics, plant physiology, and related fields
Originally published as the stand-alone Chlamydomonas Sourcebook, then expanded as the second volume in a three-part comprehensive gold-standard reference, The Chlamydomonas Sourcebook: Organellar and Metabolic Processes has been fully revised and updated to include a wealth of new knowledge and resources for the Chlamydomonas community. It details the tremendous progress recently made with respect to imaging the ultrastructure of cells, dissecting acclimation and biosynthetic responses, and elucidating molecular processes underlying the biology of organelles. In particular, this volume includes exciting new developments in the use of imaging technologies for examining supramolecular organization of the chloroplast, defining mechanisms of branched electron transfer pathways in photosynthesis, dissecting the organization of pyrenoids and CO2 concentration mechanisms, presenting the intricacies associated with acclimation to environmental conditions and providing new insights into dark metabolism and the network of fermentative metabolism. This book thus presents the latest advances in both the research and uses of new experimental approaches and technologies, making this a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, aspects of human disease, acclimation to environmental change, and the biogenesis of cellular complexes. - Describes molecular techniques, analysis of the recently sequenced genome, reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism - Provides methods for Chlamydomonas research and best practices for their applications; this includes methods for cell culture, preservation of cultures, preparation of media, lists of inhibitors, and other additives to culture media, classical genetic manipulation, and new approaches for gene transfer and editing technologies - Assists researchers with common laboratory problems such as contamination
Volumes 5A and 5B of Fundamentals of Medical Cell Biology are a treatment of several key subjects, including cellular signaling. One profitable way to tackle both volumes is to read them with care but without endeavouring to master most of the details. Then should follow a more plodding study, chapter by chapter. Such a strategy is also urged in respect of the preceding volumes. Although each chapter is only as detailed as space permits, it should in the case of the student serve as an incentive to the study of the original papers.
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as methods for studying ciliary polarity in Xenopus, analysis of signaling pathways in mammalian spermatozoa, and biochemical and physiological analysis of axonemal dyneins. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers cilia - Contains chapters on such topics as methods for studying ciliary polarity in Xenopus, analysis of signaling pathways in mammalian spermatozoa, and biochemical and physiological analysis of axonemal dyneins