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The ideal starting point for investigating, developing, and implementing stable isotope technologies. • Guides researchers through basic, tested, and proven protocols including DNA, RNA, protein, and phospholipid fatty acid (PLFA) SIP, from concept and history through detailed methodology, troubleshooting, and interpretation to optimal and future uses. • Explores important and emerging applications of SIP in environmental microbiology, ranging from bioremediation and gene mining to carbon tracking and gut microflora function. • Examines explorations of further elegant isotope labeling technologies such as Raman-FISH, NanoSIMS, and isotope arrays. • Serves as a valuable resource for environmental microbiology students and researchers and genomics, biotechnology, and medical microbiology professionals.
This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stable Isotope Probing: Methods and Protocols provides readers with up-to-date protocols ranging from basic to the most sophisticated applications of SIP and will benefit anyone pursuing this exciting area of study.
This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stable Isotope Probing: Methods and Protocols provides readers with up-to-date protocols ranging from basic to the most sophisticated applications of SIP and will benefit anyone pursuing this exciting area of study.
The use of microbial plant protection products is growing and their importance will strongly increase due to political and public pressure. World population is growing and the amount of food needed by 2050 will be double of what is produced now whereas the area of agricultural land is decreasing. We must increase crop yield in a sustainable way. Chemical plant growth promoters must be replaced by microbiological products. Also here, the use of microbial products is growing and their importance will strongly increase. A growing area of agricultural land is salinated. Global warming will increase this process. Plants growth is inhibited by salt or even made impossible and farmers tend to disuse the most salinated lands. Microbes have been very successfully used to alleviate salt stress of plants. Chemical pollution of land can make plant growth difficult and crops grown are often polluted and not suitable for consumption. Microbes have been used to degrade these chemical pollutants.
Centrifugal Separations in Molecular and Cell Biology focuses on the application of modern centrifugation technology in molecular and cell biology, including the separation and fractionation of biological particles by centrifugation on the preparative and analytical scales. The selection first covers the principles and practices of centrifugation and the bases of centrifugal separations. Discussions focus on the basic concepts of sedimentation theory, centrifugation methods, designing centrifugation experiments, care of centrifuges and rotors, and statistical estimation of molecular parameters. The book also ponders on the practical aspects of rate-zonal centrifugation, including gradient materials, density and viscosity of glycerol solutions, and resolution and gradient shape. The publication examines fractionations in zonal rotors and the quantitative aspects of rate-zonal centrifugation. The text then reviews isopycnic centrifugation in ionic media and analytical centrifugation. Topics include separation by isopycnic banding, large-scale preparative procedures, and density-gradient solutes. The selection is a valuable reference for readers interested in centrifugation technology.
Microoganisms are distributed across every ecosystem, and microbial transformations are fundamental to the operation of the biosphere. Microbial ecology is the study of this interaction between microorganisms and their environment, and arguably represents one of the most important areas of biological research. Yet for many years our study of microbial flora was severely limited: the primary method of culturing microorganisms on media allowed us to study only between 0.1 and 10% of the total microbial flora in any given environment. Molecular Microbial Ecology gives a comprehensive guide to the recent revolution in the study of microorganisms in the environment. Details are given on molecular methods for isolating some of the previously uncultured and numerically dominant microbial groups. PCR-based approaches to studying prokaryotic systematics are described, including ribosomal RNA analysis and stable isotope probing. Later chapters cover DNA hybridisation techniques (including fluorescent in situ hybridisation), as well as genomic and metagenomic approaches to microbial ecology. Gathering together some of the world’s leading experts, this book provides an invaluable introduction to the modern theory and molecular methods used in studying microbial ecology.