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In the present investigation, in vitro tuberization, pre treatments for improvement of seed germination, potting media for seedling establishment and influence of growth regulators on sprouting of tubers were studied. In the in vitro techniques, tuber node was used as explant for the micro tuber formation. Explants sterilized with 70% ethanol for 30 seconds and HgCl2 for 60 sec reduced the contamination percentage (8.00%). MS medium supplemented with 4.0 mgl-1 BAP and 1.0 mgl-1 NAA recorded the highest response for primary tuber (100 %) and secondary tuber (100 %) formation. Minimum number of days for primary (19.88 days) and secondary tuber (45.11 days) formation was recorded in the medium supplemented with 4.0 mgl-1 BAP and 1.0 mgl-1 NAA. This also recorded the maximum number of tuber (1.77) from single explant. GA3 (1.0 mgl-1) was observed to be vital for the elongation of shoot where as IAA (1.0 mgl-1) in combination with IBA was effective for induction of roots on MS medium. Seed germination is erratic due to the presence of hard seed coat and hence germination is very poor. The studies on seed treatments revealed that, pre treating the seeds with hot water for an hour improved the germination (34.88%) and also resulted in earlier germination (48.35 days) of seeds. With reference to different media, the combination of sand, soil, cocopeat (2:2:1) recorded better seed germination and early germination and was very effective for micro tuber development. At 150 days after sowing, this medium gave the maximum length of the micro tuber (2.84 cm), fresh weight (1.76 g) and dry weight (1.25 g) of the micro tuber while sand:soil:FYM recorded the maximum girth of the micro tuber (3.29 cm). Tubers treated with different growth regulators to enhance the sprouting, indicated that ethrel (500ppm) contributed for maximum sprouting percentage of tubers (100%), earlier sprouting (6.33days), maximum plant height (99.32cm) and maximum number of leaves (34.04).
Microbiome Stimulants for Crops: Mechanisms and Applications provides the latest developments in the real-world development and application of these crop management alternatives in a cost-effective, yield protective way. Sections address questions of research, development and application, with insights into recent legislative efforts in Europe and the United States. The book includes valuable information regarding mechanisms and the practical information needed to support the growing microbial inoculant and biostimulant industry, thus helping focus scientific research in new directions. - Provides methods for finding and testing endophytic and growth promotional microbes - Explains the mechanisms of microbes and other biostimulant function in promoting plant growth - Evaluates methods for treatments of plants with microbes and microbiome stimulants - Identifies areas for new research
27 chapters cover the distribution, economic importance, conventional propagation, micropropagation, tissue culture studies, and in vitro production of important medicinal and other pharmaceutical compounds in various species of Anchusa, Brucea, Catharanthus, Chrysanthemum, Coleus, Corydalis, Coreopsis, Emilia, Ginkgo, Gloriosa, Hypericum, Inonotus, Leucosceptrum, Lilium, Linum, Mosses, Nandina, Penstemon, Prunus, Pteridium, Quassia, Ribes, Senecio, Taraxacum, Thermopsis, Vanilla, and Vitiveria. Like the previous five volumes on medicinal and aromatic plants (Volumes 4, 7, 15, 21, and 24), this book contains a wealth of useful information for advanced students and researchers in the field of plant biotechnology and chemical engineering, pharmacy, botany and tissue culture.
This technical paper on ornamental plant propagation in the tropics has been written to highlight the potential of tissue culture as an advanced propagation technology allowing for mass cloning of selected varieties or ecotypes. This document provides information on the state of the art of tissue culture propagation and gives technical details for propagating some 30 ornamental plant species. The aim of the paper is to enhance the technical capacity of public institutions and private entrepreneurs, thus leading to new production and market opportunities. It also aims to facilitate and encourage the use of techniques to promote ornamental plant diversity.
Poisonous plants are used in traditional medicine systems in various healing therapies. They are a rich resource of ingredients used in herbal drug formulations that are also used in the development of synthetic drugs. They are recognized for their antioxidant, anti-inflammation, anti-cancer, and anti-diabetic activities and for many other health benefits. Exploring Poisonous Plants: Medicinal Values, Toxicity Responses, and Therapeutic Uses provides an analysis of the most important poisonous herbs, shrubs, and trees, detailing poisonous plants while demonstrating endorsements for their potential therapeutic values. Features: Presents therapeutic potentials on various poisonous herbs, shrubs, and trees. Provides descriptions of notable toxic compounds and discusses their adverse effects when consumed by animals or people. Gives practical guidance for botanical description, distribution, phytochemical constituents, pharmacological studies, and traditional and other potential uses of selected poisonous plants. This volume in the Exploring Medicinal Plants series is appropriate for scientists, researchers, and students working with poisonous plants, as well as in areas of economic botany, plant biochemistry, biotechnology, pharmacognosy, pharmaceuticals, industrial chemistry, and nanomedicine.
This volume provides summarized scientific evidence of the different classes of plant-derived phytocompounds, their sources, chemical structures, anticancer properties, mechanisms of action, methods of extraction, and their applications in cancer therapy. It also discusses endophyte-derived compounds as chemopreventives to treat various cancer types. In addition, it provides detailed information on the enhanced production of therapeutically valuable anticancer metabolites using biotechnological interventions such as plant cell and tissue culture approaches, including in vitro-, hairy root- and cell-suspension culture; and metabolic engineering of biosynthetic pathways. Anticancer Plants: Natural Products and Biotechnological Implements – Volume 2” explores the natural bioactive compounds isolated from plants as well as fungal endophytes, their chemistry, and preventive effects to reduce the risk of cancer. Moreover, it highlights the genomics/proteomics approaches and biotechnological implementations. Providing solutions to deal with the challenges involved in cancer therapy, the book benefits a wide range of readers including academics, students, and industrial experts working in the area of natural products, medicinal plant chemistry, pharmacology, and biotechnology.
This book presents a comprehensive overview of plant stresses caused by salt, drought, extreme temperatures, oxygen and toxic compounds, which are responsible for huge losses in crop yields. It discusses the latest research on the impact of salinity and global environment changes, and examines the advances in the identification and characterization of the mechanisms that allow plants to tolerate biotic and abiotic stresses. Further it presents our current understanding of metabolic fluxes and the various transporters that collectively open the possibility of applying in vitro technology and genetic engineering to improve stress tolerance. Exploring advanced methods that augment traditional plant tissue culture and breeding techniques toward the development of new crop varieties that can tolerate biotic and abiotic stresses to achieve sustainable food production, this book is a valuable resource for plant scientists and researchers.
Although a great deal of research on ornamental geophytes has been conducted since the beginning of the 1990s, current information has not been comprehensively presented to researchers and horticulturalists. Covering the latest advances in geophytes science, Ornamental Geophytes: From Basic Science to Sustainable Production provides up-to-date revi