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These proceedings contain a variety of scientific achievements and techniques presented at a 1998 international congress on plant biotechnology. Achievements today have already surpassed all previous expectations, and the field is now on the verge of creating the "evergreen revolution".
As the oldest and largest human intervention in nature, the science of agriculture is one of the most intensely studied practices. From manipulation of plant gene structure to the use of plants for bioenergy, biotechnology interventions in plant and agricultural science have been rapidly developing over the past ten years with immense forward leaps on an annual basis. This book begins by laying the foundations for plant biotechnology by outlining the biological aspects including gene structure and expression, and the basic procedures in plant biotechnology of genomics, metabolomics, transcriptomics and proteomics. It then focuses on a discussion of the impacts of biotechnology on plant breeding technologies and germplasm sustainability. The role of biotechnology in the improvement of agricultural traits, production of industrial products and pharmaceuticals as well as biomaterials and biomass provide a historical perspective and a look to the future. Sections addressing intellectual property rights and sociological and food safety issues round out the holistic discussion of this important topic. Includes specific emphasis on the inter-relationships between basic plant biotechnologies and applied agricultural applications, and the way they contribute to each other Provides an updated review of the major plant biotechnology procedures and techniques, their impact on novel agricultural development and crop plant improvement Takes a broad view of the topic with discussions of practices in many countries
This volume provides methods on different aspects and applications on plants, algae, photosynthetic bacteria, synthetic construct design, and multiplex cloning. Chapters cover multiple aspects of synthetic metabolic, photosynthetic systems, metabolic and signaling pathways, advanced engineering of metabolic networks, isolation of organelles and co-culture of microorganisms, and methods for the on command manipulation of the relative stability of proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Plant Synthetic Biology: Methods and Protocols aims to be a useful resource for both researchers starting to explore novel experimental avenues as well as for experts willing to expand their portfolio of tools and strategies.
The tremendous progress in biology over the last half century - from Watson and Crick's elucidation of the structure of DNA to today's astonishing, rapid progress in the field of synthetic biology - has positioned us for significant innovation in chemical production. New bio-based chemicals, improved public health through improved drugs and diagnostics, and biofuels that reduce our dependency on oil are all results of research and innovation in the biological sciences. In the past decade, we have witnessed major advances made possible by biotechnology in areas such as rapid, low-cost DNA sequencing, metabolic engineering, and high-throughput screening. The manufacturing of chemicals using biological synthesis and engineering could expand even faster. A proactive strategy - implemented through the development of a technical roadmap similar to those that enabled sustained growth in the semiconductor industry and our explorations of space - is needed if we are to realize the widespread benefits of accelerating the industrialization of biology. Industrialization of Biology presents such a roadmap to achieve key technical milestones for chemical manufacturing through biological routes. This report examines the technical, economic, and societal factors that limit the adoption of bioprocessing in the chemical industry today and which, if surmounted, would markedly accelerate the advanced manufacturing of chemicals via industrial biotechnology. Working at the interface of synthetic chemistry, metabolic engineering, molecular biology, and synthetic biology, Industrialization of Biology identifies key technical goals for next-generation chemical manufacturing, then identifies the gaps in knowledge, tools, techniques, and systems required to meet those goals, and targets and timelines for achieving them. This report also considers the skills necessary to accomplish the roadmap goals, and what training opportunities are required to produce the cadre of skilled scientists and engineers needed.
Now more than ever, biology has the potential to contribute practical solutions to many of the major challenges confronting the United States and the world. A New Biology for the 21st Century recommends that a "New Biology" approach-one that depends on greater integration within biology, and closer collaboration with physical, computational, and earth scientists, mathematicians and engineers-be used to find solutions to four key societal needs: sustainable food production, ecosystem restoration, optimized biofuel production, and improvement in human health. The approach calls for a coordinated effort to leverage resources across the federal, private, and academic sectors to help meet challenges and improve the return on life science research in general.
We are facing global issues concerning environmental pollution and shortages of food, feed, phytomass (plant biomass) and natural resources, which will become more serious in the forthcoming decades. To solve these issues, immeasurable numbers of various plants and huge amounts of phytomass are required every year for food, feed and for the improvement of amenities, the environment and our quality of life. Increased phytomass is also required as alternative raw material for producing bio-energy, biodegradable plastics and many other plant-originated industrial products. Only by using phytomass as a reproducible energy source and raw material, instead of fossil fuels and atomic power, we can save natural resources and minimize environmental pollution. To increase phytomass globally, we need billions of quality transplants (small plants) to be grown yearly, in the field or in the greenhouse, under various environmental conditions. However, these high quality transplants can be produced only under carefully controlled, rather than variable environment al conditions. Recent research has shown that the closed transplant production system requires considerably small amounts of electricity, water, fertilizer, CO) and pesticide to produce value-added transplants as scheduled with minimum release of environmental pollutants and minimum loss of transplants. The closed or closed-type transplant production system is defined as a transplant production system covered with opaque walls with minimized or controlled ventilation rates, using artificial lighting. With this system, photoperiod, light intensity and quality, air temperature, humidity, CO) concentration and air current speed can be controlled as desired.
Designed to inform and inspire the next generation of plant biotechnologists Plant Biotechnology and Genetics explores contemporary techniques and applications of plant biotechnology, illustrating the tremendous potential this technology has to change our world by improving the food supply. As an introductory text, its focus is on basic science and processes. It guides students from plant biology and genetics to breeding to principles and applications of plant biotechnology. Next, the text examines the critical issues of patents and intellectual property and then tackles the many controversies and consumer concerns over transgenic plants. The final chapter of the book provides an expert forecast of the future of plant biotechnology. Each chapter has been written by one or more leading practitioners in the field and then carefully edited to ensure thoroughness and consistency. The chapters are organized so that each one progressively builds upon the previous chapters. Questions set forth in each chapter help students deepen their understanding and facilitate classroom discussions. Inspirational autobiographical essays, written by pioneers and eminent scientists in the field today, are interspersed throughout the text. Authors explain how they became involved in the field and offer a personal perspective on their contributions and the future of the field. The text's accompanying CD-ROM offers full-color figures that can be used in classroom presentations with other teaching aids available online. This text is recommended for junior- and senior-level courses in plant biotechnology or plant genetics and for courses devoted to special topics at both the undergraduate and graduate levels. It is also an ideal reference for practitioners.
Woody plants belong to various taxonomic groups, which are heterogeneous in morphology, physiology, and geographic distribution. OtheJWise, they have neither strong evolutionruy relationships nor share a conunon habitat. They are a primaIy source of fiber and timber, and also include many edible fruit species. Their unique phenotypic behavior includes a perennial habit associated with extensive secondary growth. Additional characteristics of woody plants include: developmental juvenility and maturity with respect to growth habit, flowering time, and morphogenetic response in tissue cultures; environmental control of bud dormancy and flowering cycles; variable tolerance to abiotic stresses, wounding and pathogens; and long distance transport of water and IRltrients. Woody plants, particularly tree species, have been the focus of numerous physiological studies to understand their specialized functions, however, only recently they have become the target of molecular studies. Recent advances in our understanding of signal transduction pathways for environmental responses in herbaceous plants, including the identification and cloning of genes for proteins involved in signal transduction. should provide useful leads to undertake parallel studies with woody plants. Molecular mapping techniques, coupled with the availability of cloned genes from herbaceous plants, should provide shortcuts to cloning relevant genes from woody plants. The unique phenotypes of these plants can then be targeted for improvement through genetic engineering.
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