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"As will be seen, there is not much missing here. I thought that the sections were well balanced, with rarely too much or too little on a given topic...This is a text to be welcomed by both teachers and students." BIOCHEMISTRY & MOLECULAR BIOLOGY EDUCATION (on the first edition) The second edition of this successful textbook explains the basic principles behind the key techniques currently used in the modern biochemical laboratory and describes the pros and cons of each technique and compares one to another. It is non-mathematical, comprehensive and approachable for students who are not physical chemists. A major update of this comprehensive, accessible introduction to physical biochemistry. Includes two new chapters on proteomics and bioinformatics. Introduces experimental approaches with a minimum of mathematics and numerous practical examples. Provides a bibliography at the end of each chapter. Written by an author with many years teaching and research experience, this text is a must-have for students of biochemistry, biophysics, molecular and life sciences and food science.
Suitable for advanced undergraduate and graduate students in biochemistry, this book provides clear, concise, well-exampled descriptions of the physical methods that biochemists and molecular biologists use.
This first volume contains data on amino acids which consists of the coefficients of solubility in water, heat capacities, entropies of formation, and heats of combustion. Specific gravity liquids, sucrose solution, CsCI solution isokinetic glycerol and sucrose gradients for density gradient centrifugation and the temperature dependence for select compounds are included.
The objective of this book is to provide a unifying approach to the study of biophysical chemistry for the advanced undergraduate who has had a year of physics, organic chem istry, calculus, and biology. This book began as a revised edition of Biophysical Chemistry: Molecules to Membranes, which Elizabeth Simons and I coauthored. That short volume was written in an attempt to provide a concise text for a one-semester course in biophysical chemistry at the graduate level. The experience of teaching biophysical chemistry to bi ologically oriented students over the last decade has made it clear that the subject requires a more fundamental text that unifies the many threads of modem science: physics, chem istry, biology, mathematics, and statistics. This book represents that effort. This volume is not a treatment of modem biophysical chemistry with its rich history and many contro versies, although a book on that topic is also needed. The Physical Basis of Biochemistry is an introduction to the philosophy and practice of an interdisciplinary field in which biological systems are explored using the quantitative perspective of the physical scientist. I have three primary objectives in this volume: one, to provide a unifying picture of the interdisciplinary threads from which the tapestry of biophysical studies is woven; two, to provide an insight into the power of the modeling approach to scientific investigation; and three, to communicate a sense of excitement for the activity and wholesome argument that characterize this field of study.
The Present Volume Provides An Excellent Up-To-Date Account Of The Course And Development Of Biochemistry. The Text Revolves Around The Basic Principles Of Biochemistry As Well As Molecular And Biochemical Control Mechanisms Responsible For Structural, Informational And Behavioural Properties Of Cells Resulting In A Composite Picture Of Modern Biochemistry. The Book Is Organized Into Five Sections That Follows An Introductory Chapter Dealing With Biochemical Basis Of Life. Part I Treats The Foundations And Fundamentals Which Includes Four Chapters To Help Prepare A Student Finally To Enter The Realm Of Biochemistry. This Part Is Especially Designed To Clarify The Basic Concepts And Introducing Quantitation In Biochemical Sciences And Represents A Distinctive And Crucial Feature Above All The Existing Texts. Part Ii Deals With Molecular Structure And Organization Of Biomolecules. This Part Is Composed Of Five Chapters Describing In Detail, The Biochemical Organization Of Information Molecules In Living Cells. Part Iii Represents Biosynthesis Of Biomolecules Covering Five Chapters Describing In Detail The Biochemical Control Of Traffic Of Information Molecules In The Living Cells. Part Iv Describes The Dynamics Of Energy Transformation And Represents Six Chapters Dealing With The Maintenance And Expenditure Of Energy In Biochemical Processes. Part V Deals With Enzymology And Special Aspects Of Metabolism And Includes Eleven Chapters Especially Devoted To Cover Recent Developments And Topics Of Current Importance. This Volume Concludes With A Chapter Dealing With The Place And Relevance Of Biochemistry On An Interdisciplinary Platform. Every Chapter Is Followed By A Short List Of Selected General References Pertaining To That Particular Subject. There Are Seven Indices Following The Complete Write Up And Is Primarily Directed Towards Providing Additional And Extra Information Useful To Establish A Link Between All The Sections And Chapters.In View Of Academic Curriculum Existing In Various Universities And Institutions (Including Agricultural Universities), The Present Book Should Conform To The Requirements And Interests Of Honours And Postgraduate Students In Life Sciences In General Including Biochemistry, Botany, Zoology, Genetics And Microbiology.
This text surveys the principal physical approaches used to characterize the structure and function of biomacromolecules such as proteins and DNA. It covers spectroscopy, chromatography, mass spectrometry and other topics.
The Second Edition of Principles of Physical Biochemistry provides the most current look at the theory and techniques used in the study of the physical chemistry of biological and biochemical molecules--including discussion of mass spectrometry and single-molecule methods. As leading experts in biophysical chemistry, these well-known authors offer unique insights and coverage not available elsewhere. Physical techniques currently used by practicing biochemists, including new chapters dedicated to extended material on mass spectrometry and single-molecule methods are included. The book's streamlined organization groups all hydrodynamic methods in Chapter 5 and combines Raman spectroscopy with the spectroscopy section. Relevant problems and applications help readers develop critical-thinking skills that they can apply to real biochemical and biological situations facing professionals in the industry. Biological Macromolecules; Thermodynamics and Biochemistry; Molecular Thermodynamics; Statistical Thermodynamics; Methods for the Separation and Characterization of Macromolecules; X-Ray Diffraction; Scattering From Solutions of Macromolecules; Quantum Mechanics and Spectros© Absorption Spectros© Linear and Circular Dichroism; Emission Spectros© Nuclear Magnetic Resonance Spectros© Macromolecules in Solution: Thermodynamics and Equilibria; Chemical Equilibria Involving Macromolecules; Mass Spectrometry of Macromolecules; Single-Molecule Methods. A useful reference for biochemistry professionals or for anyone interested in learning more about biochemistry.
This volume completes the account of physical methods used in biochemistry that was covered in Volume 11A, which was published in 1985. It deals with fast reaction methods, high performance liquid chromatography of peptides and proteins, fluorescence spectroscopy, neutron and X-ray scattering techniques, and also Raman and resonance spectroscopy which have in recent years been used to an increasing extent in biochemistry. This addition to the invaluable series New Comprehensive Biochemistry will be of interest to all those working in the fields of biochemistry and biophysics.
Exercise Biochemistry, Second Edition, offers a clear explanation of how exercise affects molecular-level functioning in athletes and nonathletes, both healthy and diseased.