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This book summarizes recent advances made in the biophysics, biochemistry, and molecular biology of the enzyme known as Photosystem I, the light-induced plastocyanin: ferredoxin oxidoreductase. The volume provides a unique compilation of chapters that includes information highlighting controversial issues to indicate the frontiers of research and places special emphasis on methodology and practice for new researchers.
The most mysterious part of photosynthesis yet the most important for all aerobic life on Earth (including ourselves) is how green plants, algae and cyanobacteria make atmospheric oxygen from water. This thermodynamically difficult process is only achieved in Nature by the unique pigment/protein complex known as Photosystem II, using sunlight to power the reaction. The present volume contains 34 comprehensive chapters authored by 75 scientific experts from around the world. It gives an up-to-date account on all what is currently known about the molecular biology, biochemistry, biophysics and physiology of Photosystem II. The book is divided into several parts detailing the protein constituents, functional sites, tertiary structure, molecular dynamics, and mechanisms of homeostasis. The book ends with a comparison of Photosystem II with other related enzymes and bio-mimetic systems. Since the unique water-splitting chemistry catalyzed by Photosystem II leads to the production of pure oxygen gas and has the potential for making hydrogen gas, a primary goal of this book is to provide a molecular guide to future protein engineers and bio-mimetic chemists in the development of biocatalysts for the generation of clean, renewable energy from sunlight and water.
This book is a tribute to three outstanding scientists, Professors Jan Anderson FRS, Leslie Dutton FRS and John Walker FRS, Nobel Laureate. Covering some of the most recent advances in the fields of Bioenergetics and Photosynthesis, this book is a compilation of contributions from leading scientists actively involved in understanding the natural biological processes associated with the flow of energy in biological cells. The lectures found in this significant volume were presented at a meeting in March 2016 in Singapore to commemorate the outstanding research in this area.The contents begin with the ideas, specially the contribution from Nobel Laureate Rudolph Marcus, who is well-known for creating the theory of electron transport reactions. This is followed by contributions of many others on various aspects of respiratory and photosynthetic transport chains as well as the dynamic regulation of light harvesting and electron transport events in oxygenic photosynthesis. The book is highly recommended to postgraduate students and researchers who are interested in various aspects of bioenergetic cycles.
Molecular biology, particularly molecular genetics, is among the newest and most powerful approach in modern photosynthesis research. Development of molecular biology techniques has provided new methods to solve old problems in many biological disciplines. Molecular biology has its greatest potential for contribution when applied in combination with other disciplines, to focus not just on genes and molecules, but on the complex interaction between them and the biochemical pathways in the whole organism. Photosynthesis is surely the best studied research area in plant biology, making this field the foremost candidate for successfully employing molecular genetic techniques. Already, the success of molecular biology in photosynthesis has been nothing short of spectacular. Work performed over the last few years, much of which is sum marized in this volume, stands in evidence. Techniques such as site-specific mutagenesis have helped us in examining the roles of individual protein domains in the function of multiunit complexes such as the enzyme ribulose-l ,5-bisphos phate carboxylase/oxygenase (RUBISCO) and the oxygen evolving photo system (the photosystem II). The techniques of molecular biology have been very important in advancing the state of knowledge of the reaction center from the photosynthetic bacteria whose structure has been elegantly deduced by H. Michel and 1. Deisenhofer from the X-ray studies of its crystals.
Structure and function of the components of the photosynthetic apparatus and the molecular biology of these components have become the dominant themes in advances in our understanding of the light reactions of oxygenic photosynthesis. Oxygenic Photosynthesis: The Light Reactions presents our current understanding of these reactions in thylakoid membranes. Topics covered include the photosystems, the cytochrome b6-f complex, plastocyanin, ferredoxin, FNR, light-harvesting complexes, and the coupling factor. Chapters are also devoted to the structure of thylakoid membranes, their lipid composition, and their biogenesis. Updates on the crystal structures of cytochrome f, ATP synthase and photosystem I are presented and a section on molecular biology and evolution of the photosynthetic apparatus is also included. The chapters in this book provide a comprehensive overview of photosynthetic reactions in eukaryotic thylakoids. The book is intended for a wide audience, including graduate students and researchers active in this field, as well as those individuals who have interests in plant biochemistry and molecular biology or plant physiology.
Photosynthesis has been an important field of research for more than a century, but the present concerns about energy, environment and climate have greatly intensified interest in and research on this topic. Research has progressed rapidly in recent years, and this book is an interesting read for an audience who is concerned with various ways of harnessing solar energy.Our understanding of photosynthesis can now be said to have reached encyclopedic dimensions. There have been, in the past, many good books at various levels. Our book is expected to fulfill the needs of advanced undergraduate and beginning graduate students in branches of biology, biochemistry, biophysics, and bioengineering because photosynthesis is the basis of future advances in producing more food, more biomass, more fuel, and new chemicals for our expanding global human population. Further, the basics of photosynthesis are and will be used not only for the above, but in artificial photosynthesis, an important emerging field where chemists, researchers and engineers of solar energy systems will play a major role.
Photosynthesis: Photobiochemistry and Photobiophysics is the first single-authored book in the Advances in Photosynthesis Series. It provides an overview of the light reactions and electron transfers in both oxygenic and anoxygenic photosynthesis. The scope of the book is characterized by the time frame in which the light reactions and the subsequent electron transfers take place, namely between =10sup-12/sup and =10-3 second. The book is divided into five parts: An Overview; Bacterial Photosynthesis; Photosystem II & Oxygen Evolution; Photosystem I; and Proton Transport and Photophosphorylation. In discussing the structure and function of various protein complexes, we begin with an introductory chapter, followed by chapters on light-harvesting complexes, the primary electron donors and the primary electron acceptors, and finally the secondary electron donors. The discussion on electron acceptors is presented in the order of their discovery to convey a sense of history, in parallel with the advancement in instrumentation of increasing time resolution. The book includes a large number of stereo pictures showing the three-dimensional structure of various photosynthetic proteins, which can be easily viewed with unaided eyes. This book is designed to be used as a textbook in a graduate or upper-division undergraduate course in photosynthesis, photobiology, plant physiology, biochemistry, and biophysics; it is equally suitable as a resource book for students, teachers, and researchers in the areas of molecular and cellular biology, integrative biology, microbiology, and plant biology.
Dr. Harris has played a major role in the development of this organism as a model system. Her previous version of the Chlamydomonas Sourcebook which published in 1989, has been a classic in the field and is considered required reading for anyone working with this organism. This latest edition has been expanded to include three volumes providing molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism. Methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media, are included. Additions to this volume also include help with common laboratory problems such as contamination, student demonstrations, and properties of particular strains and mutants. This volume is part of a 3-Volume Set (ISBN: 978-0-12-370873-1) and is also sold individually. - Expanded revision of gold standard reference - Includes latest advances in research, including completion of the genome - Provides broad perspective with studies in cell and molecular biology, genetics, plant physiology and related fields - Available as part of a 3-Volume Set or sold individually
This book covers the expression of photosynthesis related genes including regulation both at transcriptional and translational levels. It reviews biogenesis, turnover, and senescence of thylakoid pigment protein complexes and highlights some crucial regulatory steps in carbon metabolism.