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Biomedical photonics is currently one of the fastest growing fields, connecting research in physics, optics, and electrical engineering coupled with medical and biological applications. It allows for the structural and functional analysis of tissues and cells with resolution and contrast unattainable by any other methods. However, the major challenges of many biophotonics techniques are associated with the need to enhance imaging resolution even further to the sub-cellular level as well as translate them for in vivo studies. The tissue optical clearing method uses immersion of tissues into optical clearing agents (OCAs) that reduces the scattering of tissue and makes tissue more transparent and this method has been successfully used ever since. This book is a self-contained introduction to tissue optical clearing, including the basic principles and in vitro biological applications, from in vitro to in vivo tissue optical clearing methods, and combination of tissue optical clearing and various optical imaging for diagnosis. The chapters cover a wide range of issues related to the field of tissue optical clearing: mechanisms of tissue optical clearing in vitro and in vivo; traditional and innovative optical clearing agents; recent achievements in optical clearing of different tissues (including pathological tissues) and blood for optical imaging diagnosis and therapy. This book provides a comprehensive account of the latest research and possibilities of utilising optical clearing as an instrument for improving the diagnostic effectiveness of modern optical diagnostic methods. The book is addressed to biophysicist researchers, graduate students and postdocs of biomedical specialties, as well as biomedical engineers and physicians interested in the development and application of optical methods in medicine. Key features: The first collective reference to collate all known knowledge on this topic Edited by experts in the field with chapter contributions from subject area specialists Brings together the two main approaches in immersion optical clearing into one cohesive book
Biomedical optical imaging is a rapidly emerging research area with widespread fundamental research and clinical applications. This book gives an overview of biomedical optical imaging with contributions from leading international research groups who have pioneered many of these techniques and applications. A unique research field spanning the microscopic to the macroscopic, biomedical optical imaging allows both structural and functional imaging. Techniques such as confocal and multiphoton microscopy provide cellular level resolution imaging in biological systems. The integration of this technology with exogenous chromophores can selectively enhance contrast for molecular targets as well as supply functional information on processes such as nerve transduction. Novel techniques integrate microscopy with state-of-the-art optics technology, and these include spectral imaging, two photon fluorescence correlation, nonlinear nanoscopy; optical coherence tomography techniques allow functional, dynamic, nanoscale, and cross-sectional visualization. Moving to the macroscopic scale, spectroscopic assessment and imaging methods such as fluorescence and light scattering can provide diagnostics of tissue pathology including neoplastic changes. Techniques using light diffusion and photon migration are a means to explore processes which occur deep inside biological tissues and organs. The integration of these techniques with exogenous probes enables molecular specific sensitivity.
This third edition of the biomedical optics classic Tissue Optics covers the continued intensive growth in tissue optics—in particular, the field of tissue diagnostics and imaging—that has occurred since 2007. As in the first two editions, Part I describes fundamentals and basic research, and Part II presents instrumentation and medical applications. However, for the reader’s convenience, this third edition has been reorganized into 14 chapters instead of 9. The chapters covering optical coherence tomography, digital holography and interferometry, controlling optical properties of tissues, nonlinear spectroscopy, and imaging have all been substantially updated. The book is intended for researchers, teachers, and graduate and undergraduate students specializing in the physics of living systems, biomedical optics and biophotonics, laser biophysics, and applications of lasers in biomedicine. It can also be used as a textbook for courses in medical physics, medical engineering, and medical biology.
This entry-level textbook, covering the area of tissue optics, is based on the lecture notes for a graduate course (Bio-optical Imaging) that has been taught six times by the authors at Texas A&M University. After the fundamentals of photon transport in biological tissues are established, various optical imaging techniques for biological tissues are covered. The imaging modalities include ballistic imaging, quasi-ballistic imaging (optical coherence tomography), diffusion imaging, and ultrasound-aided hybrid imaging. The basic physics and engineering of each imaging technique are emphasized. A solutions manual is available for instructors; to obtain a copy please email the editorial department at [email protected].
Adaptive Optics for Biological Imaging brings together groundbreaking research on the use of adaptive optics for biological imaging. The book builds on prior work in astronomy and vision science. Featuring contributions by leaders in this emerging field, it takes an interdisciplinary approach that makes the subject accessible to nonspecialists who
This book describes the Optical Immersion Clearing method and its application to acquire information with importance for clinical practice and various fields of biomedical engineering. The method has proved to be a reliable means of increasing tissue transparency, allowing the investigator or surgeon to reach deeper tissue layers for improved imaging and laser surgery. This result is obtained by partial replacement of tissue water with an active optical clearing agent (OCA) that has a higher refractive index and is a better match for the refractive index of other tissue components. Natural tissue scattering is thereby reduced. An exponential increase in research using this method has occurred in recent years, and new applications have emerged, both in clinical practice and in some areas of biomedical engineering. Recent research has revealed that treating ex vivo tissues with solutions containing active OCAs in different concentrations produces experimental data to characterize drug delivery or to discriminate between normal and pathological tissues. The obtained drug diffusion properties are of interest for the pharmaceutical and organ preservation industry. Similar data can be estimated with particular interest for food preservation. The free water content evaluation is also of great interest since it facilitates the characterization of tissues to discriminate pathologies. An interesting new application that is presented in the book regards the creation of two optical windows in the ultraviolet spectral range through the application of the immersion method. These induced transparency windows open the possibility to diagnose and treat pathologies with ultraviolet light. This book presents photographs from the tissues we have studied and figures that represent the experimental setups used. Graphs and tables are also included to show the numerical results obtained in the sequential calculations performed.
This text begins by describing the basic principles and diagnostic applications of optical techniques based on detecting and processing the scattering, fluorescence, FT IR, and Raman spectroscopic signals from various tissues, with an emphasis on blood, epithelial tissues, and human skin. The second half of the volume discusses specific imaging technologies, such as Doppler, laser speckle, optical coherence tomography (OCT), and fluorescence and photoacoustic imaging.
This text draws together the fields of optical microscopy and optical data storage, in a unique compilation of valuable and novel scientific work that is scarcely to be found elsewhere. The contributing authors are unquestioned leaders of their respective fields.
Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical a
This book provides foundational information on modeling light propagation through diffusive media, with special emphasis on biological tissue. A summary of the theoretical background on light propagation through diffusive media is provided with the aid of easy-to-use software designed to calculate the solutions of the diffusion equation. The book also provides: the basic theory of photon transport with the analytical solutions of the diffusion equation for several geometries; detailed coverage of the radiative transfer equation and the diffusion equation; the theories and the formulae based on the diffusion equation that have been widely used for biomedical applications; the general concepts and the physical quantities necessary to describe light propagation through absorbing and scattering media; and, a description of the software provided on the CD-ROM, along with the accuracy of the presented solutions. Although the theoretical and computational tools provided with this book and CD-ROM have their primary use in the field of biomedical optics, there are many other applications in which they can be used, including agricultural products, forest products, food products, plastic materials, pharmaceutical products, and many others.