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The success of Assisted Reproductive Technology is critically dependent upon the use of well optimized protocols, based upon sound scientific reasoning, empirical observations and evidence of clinical efficacy. Recently, the treatment of infertility has experienced a revolution, with the routine adoption of increasingly specialized molecular biological techniques and advanced methods for the manipulation of gametes and embryos. This textbook – inspired by the postgraduate degree program at the University of Oxford – guides students through the multidisciplinary syllabus essential to ART laboratory practice, from basic culture techniques and micromanipulation to laboratory management and quality assurance, and from endocrinology to molecular biology and research methods. Written for all levels of IVF practitioners, reproductive biologists and technologists involved in human reproductive science, it can be used as a reference manual for all IVF labs and as a textbook by undergraduates, advanced students, scientists and professionals involved in gamete, embryo or stem cell biology.
The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field Includes a brief history and overview on CLEM methods
Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.
In Confocal Microscopy Methods and Protocols, Stephen Paddock and a highly skilled panel of experts lead the researcher using confocal techniques from the bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, nontechnical style, the methods specifically cover most of the commonly used model organisms: worms, sea urchins, flies, plants, yeast, frogs, and zebrafish. Centered in the many biological applications of the confocal microscope, the book makes possible the successful imaging of both fixed and living specimens using primarily the laser scanning confocal microscope. The powerful hands-on methods collected in Confocal Microscopy Methods and Protocols will help even the novice to produce first-class cover-quality confocal images.
Offers a comprehensive guide to assisted reproductive technology surveillance, describing its history, global variations, and best practices.
Reprint of the original, first published in 1887.
An essential, practical guide for clinicians treating patients with infertility.
Invertebrate Embryology and Reproduction deals with the practical and theoretical objectives of the descriptive embryology of invertebrates, along with discussions on reproduction in these groups of animals. It explains several morphological and anatomical expressions in the field and covers the embryology of invertebrate animals, starting from the Protozoa, to the Echinodermata, the Protochordate and Tunicates. These groups include economically important aquatic invertebrates, such as crustaceans, as well as medically important invertebrates and economic arthropods. Each chapter is preceded by the taxonomy of the discussed phylum and/or the species to enable the reader to locate the systematic position. - Covers phylum definition, general characteristics, classification, reproduction, agametic reproduction, gametic reproduction, spawning, fertilization, development and embryogenesis - Includes recent findings in the area, along with detailed figures and photos that illustrate important concepts - Brings together difficult-to-obtain research data from the field, not only in Egyptian libraries, but globally, and previously only found through specialized references not widely available - Clarifies descriptions with striking photos and electron microscopical studies of different species