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Histological techniques form the basis of many areas of research, yet they can often be poorly understood. Aimed at postgraduate students and those at an early stage of their career, this title provides a detailed and comprehensive introduction to histological techniques. With detailed images and slides, this book provides a unique overview of the area while providing the reader with a guide to how to use and incorporate histological techniques within their own research. Written by experts working within the field, this book is an essential handbook for anyone wanting to learn more about histological methods and how to apply them successfully.
Handbook of Microscopy is a manual that deals mainly with the basic instruments and techniques used in light microscopy and its biological applications. A large section is devoted to the study of organic matter in microfossils preserved in rocks, in view of its stratigraphic importance in mining and oil prospecting. This text is comprised of six chapters; the first of which introduces the reader to the basic principles as well as to the instruments and techniques used in light microscopy. This book also discusses the microscopes and electronic flashlights for photomicrography, along with the use of monochromatic light, stereological and physicochemical microanalysis, microanalysis by electron microscopy, and microdetermination of physical values. Attention then turns to staining and impregnation and methods of fixation, examination, cutting, and mounting. The remaining chapters focus on the microscopy of topological stains and non-specific cytological stains, with emphasis on special methods used in animal and plant histology and protistology and mycological methods in pathology. This book is written specifically for microscopists.
Insects, and their close relatives, the arachnids, centipedes, millipedes and woodlice, make ideal material for study by the recreational microscopist. Moreover for the entomologist, the addition of the use of the microscope to their tool kit adds a whole new dimension to their study, revealing in finest detail the appearance and structure of these tiny creatures. This book reveals the basics of insect microscopy, explaining what equipment is needed and how to get the best out of it. Topics covered include insects and their relatives; trapping insects for study; dissection, slide mounting, and publishing your work. This fascinating guide to the basics of insect microscopy will make ideal material for study by the recreational microscopist and will be of great interest to science students and entomologists. Beautifully illustrated with 140 colour photographs.
The coastal and ocean ecosystem is a significant feature of our planet and provides a source of food for much of life on Earth. Millions of species have been, and are still being discovered in the world’s oceans. Among these zooplankton serve as secondary producers and are significant as they form pelagic food links and act as indicators of water masses. They constitute the largest and most reliable source of protein for most of the ocean’s fishes. As such, their absence or depletion often affects fishery. In many countries, the decline in fishery has been attributed to reduced plankton populations. Furthermore, trillions of tiny copepods produce countless faecal pellets contributing greatly to the marine snow and therefore accelerating the flow of nutrients and minerals from the surface waters to the seabed. They are phylogenetically highly successful groups in terms of phylogenetic age, number of living species and success of adaptive radiation. A study of the basic and applied aspects of zooplankton would provide an index of the fishery potential and applications, offering insights into ocean ecology to safeguard food supplies and livelihoods of the millions of people living in coastal areas. For this reason, we need to understand all the facets of zooplankton as well as their interactions with atmosphere and other life forms, including human. In this context, this book discusses the basic and applied aspects of zooplankton, especially taxonomy, mosquitocidal activity, culture, analysis of nutritional, pigments and enzyme profile, preservation of copepods eggs, bioenrichment of zooplankton and application of zooplankton in sustainable aquaculture production, focusing on novel biofloc-copefloc technologies, and the impact of acidification and microplastics on zooplankton. Offering a comprehensive overview of the current issues and developments in the field of environmental and commercial applications, this book is a valuable resource for researchers, aquaculturists, environmental mangers wanting to understand the importance of zooplankton and develop technologies for the sustainable production of fish and other commodities to provide food and livelihoods for mankind.
Most neurobiological research is performed on vertebrates, and it is only natural that most texts describing neuroanatomical methods refer almost exclusively to this Phylum. Nevertheless, in recent years insects have been studied intensively and are becoming even more popular in some areas of research. They have advantages over vertebrates with respect to studying genetics of neuronal development and with respect to studying many aspects of integration by uniquely identifiable nerve cells. Insect central nervous system is characterized by its compactness and the rather large number of nerve cells in a structure so small. But despite their size, parts of the insect eNS bear structural comparisons with parts of vertebrate eNS. This applies particularly to the organization of the thoracic ganglia (and spinal cord), to the insect and vertebrate visual sys tems and, possibly, to parts of the olfactory neuropils. The neurons that make up these areas in insects are often large enough to be impaled by microelectrodes and can be injected with dyes. Added to advantages of using a small eNS, into which the sensory periphery is precisely mapped, are the many aspects of insect behaviour whose components can be quan titized and which may find both structural and functional correlates within clearly defined regions of neuropil. Together, these various features make the insect eNS a rewarding object for study. This volume is the first of two that describe both classic and recent methods for neuroanatomical research on insect eNS.
Invertebrate Embryology and Reproduction deals with the practical and theoretical objectives of the descriptive embryology of invertebrates, along with discussions on reproduction in these groups of animals. It explains several morphological and anatomical expressions in the field and covers the embryology of invertebrate animals, starting from the Protozoa, to the Echinodermata, the Protochordate and Tunicates. These groups include economically important aquatic invertebrates, such as crustaceans, as well as medically important invertebrates and economic arthropods. Each chapter is preceded by the taxonomy of the discussed phylum and/or the species to enable the reader to locate the systematic position. - Covers phylum definition, general characteristics, classification, reproduction, agametic reproduction, gametic reproduction, spawning, fertilization, development and embryogenesis - Includes recent findings in the area, along with detailed figures and photos that illustrate important concepts - Brings together difficult-to-obtain research data from the field, not only in Egyptian libraries, but globally, and previously only found through specialized references not widely available - Clarifies descriptions with striking photos and electron microscopical studies of different species