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Handbook of Methods in Aquatic Microbial Ecology is the first comprehensive compilation of 85 fundamental methods in modern aquatic microbial ecology. Each method is presented in a detailed, step-by-step format that allows readers to adopt new methods with little difficulty. The methods represent the state of the art, and many have become standard procedures in microbial research and environmental assessment. The book also presents practical advice on how to apply the methods. It will be an indispensable reference for marine and freshwater research laboratories, environmental assessment laboratories, and industrial research labs concerned with microbial measurements in water.
This book places the main actors in environmental microbiology, namely the microorganisms, on center stage. Using the modern approach of 16S ribosomal RNA, the book looks at the taxonomy of marine and freshwater bacteria, fungi, protozoa, algae, viruses, and the smaller aquatic animals such as nematodes and rotifers, as well as at the study of unculturable aquatic microorganisms (metagenomics). The peculiarities of water as an environment for microbial growth, and the influence of aquatic microorganisms on global climate and global recycling of nitrogen and sulphur are also examined. The pollution of water is explored in the context of self-purification of natural waters. Modern municipal water purification and disease transmission through water are discussed. Alternative methods for solid waste disposal are related to the economic capability of a society. Viruses are given special attention. By focusing on the basics, this primer will appeal across a wide range of disciplines.
Basic techniques; Sampling methods; Determination of biomass; Isolation methods; Identification; Specialized environments; Bacteria of fish; Bacteria of aquatic invertebrates; Epiphytic bacteria; Deep-sea bacteria; Specialized groups; Anoxygenic phototrophic bacteria; Cyanobacteria: isolation, interactions and ecology; Sulphate-reducing bacteria; Methods of studying methanogenic bacteria and methanogenic activities in aquatic environments; Activity; Assessment of bacterial activity; Nitrate metabolism by aquatic bacteria; Methods for the study of bacterial attachment.
The second edition of Microbiology of Waterborne Diseases describes the diseases associated with water, their causative agents and the ways in which they gain access to water systems. The book is divided into sections covering bacteria, protozoa, and viruses. Other sections detail methods for detecting and identifying waterborne microorganisms, and the ways in which they are removed from water, including chlorine, ozone, and ultraviolet disinfection. The second edition of this handbook has been updated with information on biofilms and antimicrobial resistance. The impact of global warming and climate change phenomena on waterborne illnesses are also discussed. This book serves as an indispensable reference for public health microbiologists, water utility scientists, research water pollution microbiologists environmental health officers, consultants in communicable disease control and microbial water pollution students. Focuses on the microorganisms of most significance to public health, including E. coli, cryptosporidium, and enterovirus Highlights the basic microbiology, clinical features, survival in the environment, and gives a risk assessment for each pathogen Contains new material on antimicrobial resistance and biofilms Covers drinking water and both marine and freshwater recreational bathing waters
Microbiological Examination Methods of Food and Water (2nd edition) is an illustrated laboratory manual that provides an overview of current standard microbiological culture methods for the examination of food and water, adhered to by renowned international organizations, such as ISO, AOAC, APHA, FDA and FSIS/USDA. It includes methods for the enumeration of indicator microorganisms of general contamination, indicators of hygiene and sanitary conditions, sporeforming, spoilage fungi and pathogenic bacteria. Every chapter begins with a comprehensive, in-depth and updated bibliographic reference on the microorganism(s) dealt with in that particular section of the book. The latest facts on the taxonomic position of each group, genus or species are given, as well as clear guidelines on how to deal with changes in nomenclature on the internet. All chapters provide schematic comparisons between the methods presented, highlighting the main differences and similarities. This allows the user to choose the method that best meets his/her needs. Moreover, each chapter lists validated alternative quick methods, which, though not described in the book, may and can be used for the analysis of the microorganism(s) dealt with in that particular chapter. The didactic setup and the visualization of procedures in step-by-step schemes allow the user to quickly perceive and execute the procedure intended. Support material such as drawings, procedure schemes and laboratory sheets are available for downloading and customization. This compendium will serve as an up-to-date practical companion for laboratory professionals, technicians and research scientists, instructors, teachers and food and water analysts. Alimentary engineering, chemistry, biotechnology and biology (under)graduate students specializing in food sciences will also find the book beneficial. It is furthermore suited for use as a practical/laboratory manual for graduate courses in Food Engineering and Food Microbiology.
Handbook of Methods in Aquatic Microbial Ecology is the first comprehensive compilation of 85 fundamental methods in modern aquatic microbial ecology. Each method is presented in a detailed, step-by-step format that allows readers to adopt new methods with little difficulty. The methods represent the state of the art, and many have become standard procedures in microbial research and environmental assessment. The book also presents practical advice on how to apply the methods. It will be an indispensable reference for marine and freshwater research laboratories, environmental assessment laboratories, and industrial research labs concerned with microbial measurements in water.
The third edition of this bestselling text has been rigorously updated to reflect major new discoveries and concepts since 2011, especially progress due to extensive application of high-throughput sequencing, single cell genomics and analysis of large datasets. Significant advances in understanding the diversity and evolution of bacteria, archaea, fungi, protists, and viruses are discussed and their importance in marine processes is explored in detail. Now in full colour throughout, all chapters have been significantly expanded, with many new diagrams, illustrations and boxes to aid students’ interest and understanding. Novel pedagogy is designed to encourage students to explore current high-profile research topics. Examples include the impacts of rising CO2 levels on microbial community structure and ocean processes, interactions of microbes with plastic pollution, symbiotic interactions, and emerging diseases of marine life. This is the only textbook addressing such a broad range of topics in the specific area of marine microbiology, now a core topic within broader Marine Science degrees. A Companion Website provides additional online resources for instructors and students, including a summary of key concepts and terminology for each chapter, links to further resources, and flashcards to aid self-assessment.
Microbial ecology is one of the fastest growing fields of microbiology. This practical volume is the bench and field scientist's guide to well-established techniques for investigating microbial communities. Both for biologists just entering the field and for experienced researchers wishingto explore new areas, this book provides the theoretical background, detailed protocols, and tips from experts for working in this field. Chapters on bacteria with interesting metabolic traits are augmented with chapters on molecular techniques, lipid analysis, and appropriate sampling techniques.The final section includes up-to-date information on biofilm development and study, the science and practice of bioremediation, modeling of biological systems (including the most useful statistical parameters), and the study of phylogenetics.