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The cultivation of diazotrophic microorganisms. Measurement of nitrogen fixation by direct means. Measurement of nitrogen fixation by indirect means. Methods for legumes in glasshouses and controlled environment cabinets. Non-legumes nodule systems. Methods for studying nitrogenase. Methods for studying enzymes involved in metabolism related to nitrogenase. Preparation and experimental use of leghaemoglobin. Methods for identifying strains of diazotrophs. Genetic studies with diazotrophs. Experiments with crop and pasture legumes: principles and practice. Production and quality control of legume inoculants. Forage grasses and grain crops. Nitrogen fixation in natural plant communities and soils. Sytems involving blue-green algae (cyanobacteria).
Silvicultural systems and biological nitrogen fixation; Morphology of nitrogen fixers in forest ecosystems; Taxonomy and distribution of non-legume nitrogen-fixing systems; Isolation and culture of nitrogen-fixing organisms; Wheeler, biochemical, physiological and environmental aspects of symbiotic nitrogen fixation; Analysis of nitrogen fixation; Agricultural and horticultural systems: implications for forestry; Nitrogen fixing plants in forest plantation management; Nitrogen fixation in North American forestry: research and application; Application of biological nitrogen fixation in European silviculture; Nitrogen fixation in Southeast Asian forestry: research and practice; Biological nitrogen fixation in forestry: research and practice in Australia and New Zealand.
Proceedings of a Workshop, ICARDA, Syria, April 14-17, 1986.
Phylogenetic classification of nitrogen-fixing organisms. Physiology of nitrogen fixation in free-living heterotrophs. Nitrogen fixation by photosynthetic bacteria. Nitrogen fixation in cyanobacteria. Nitrogen fixation by methanogenic bacteria. Associative nitrogen-fixing bacteria. Actinorhizal symbioses. Ecology of bradyrhizobium and rhizobium. The rhizobium infection process. Physiology of nitrogen-fixing legume nodules: compartments, and functions. Hydrogen cycling in symbiotic bacteria. Evolution of nitrogen-fixing symbioses. The rhizobium symbiosis of the nonlegume parasponia. Genetic analysis of rhizobium nodulation. Nodulins in root nodule development. Plant genetics of symbiotic nitrogen fixation. Molecular genetics of bradyrhizobium symbioses. The enzymology of molybdenum-dependent nitrogen fixation. Alternative nitrogen fixation systems. Biochemical genetics of nitrogenase. Regulation of nitrogen fixation genes in free-living and symbiotic bacteria. Isolated iron-molybdenum cofactor of nitrogenase.
Biochemical Methods Are Used In All Branches Of Biological Sciences And Agriculture Is No Exception. Research In Various Branches Of Agriculture Viz. Plant Physiology, Plant Pathology, Agricultural Microbiology Seed Technology Plant Genetics And Entomology Requires One Or The Other Biochemical Methods. A Researcher Has To Refer Many Journals And Books Before He Could Get To The Right Procedure For His Experiment. This Book On Biochemical Methods Attempts To Give Often Used Methods In A Single VolumeThe Book, Divided Into 13 Chapters Contains 115 Procedures. The Chapters Are Carbohydrates, Lipids, Proteins, Nucleic Acids, Vitamins, Enzymes, Nitrogen Fixation Antinutritional Factors, Plant Hormones, Pigments, Phenols Cell Fractionation And Separation Techniques. Each Procedure Is Divided Into Introduction, Principle, Materials, Procedure And Calculation. At The End Of Each Procedure References For Additional Reading Are Provided. Important Precautions, Warnings And Tips Are Given In The Notes Section.The Methods Elaborated In The Book Will Be Useful For Conducting Practical Classes At The Undergraduate And Postgraduate Levels In Science Colleges And Universities. This Manual Will Be A Bonanza For The Research Workers In Plant Sciences Since It Includes Procedures From The Classical Microkjeldahl Nitrogen Estimation To The Modern Southern Blotting Technique.
Biological nitrogen fixation in tropical agrosystems: twenty years of biological nitrogen fixation research in Africa; Sustainable agriculture: definition and measurement; Biological nitrogen fixation systems in tropical ecosystems: an overview; A protocol for screening legumes as soil-improving crops; The sustenance of tropical agriculture with multipurpose azolla; Facteurs pedoclimatiques limitant la lixation biologique l'azole; Response of some tropical nitrogen-fixing woody legumes to drought and inoculation with mycorrhiza; Improvement to the Phaseolus/Rhizobium symbiosis, with particular reference to the Caribbean region; Effect of pest management systems on biological nitrogen fixation; Agronomic evaluation of a rock phosphate as a phosphorus source for Leucaena leucocephala grown on an utisol; Nodulation of soybean grown under field conditions and inoculated with Bradyrhizobium japonicum strains; Effect of fertilization and Rhizobium inoculation on the growth of Leucaena and Gliricidia on an alfisol in south-western Nigeria; Early growth and nodulation in Leucaena and Gliricidia and the effects or pruning on biomass productivity; Comparative stude on the growth and productivity of Sesbania and Leucaena in the Central Plateau region, Rwanda; Supernoculation and non-nodulation mutants of soybean; Genetically improved rhizobia and their use in agriculture; Sustainability of nitrogen-fixing cropping systems: Nodulation and nitrogen fixation and transfer in a cowpea/rice cropping system; The role of legumes in sustaiing soil productivity and controlling soil erosion; Fitting soil-improving legumes into inland valley rice-baes cropping systems in West Africa; Herbage yield and soil fertility restoration potential of some tropical forage legumes.
Biological nitrogen fixation. Why, when an how to measure nitrogen fixation. Analysis of nitrogen. Nitrogen Balance Method. Nitrogen Difference method. Ureide (N solute) metode. N-isotopic methods. N-abundance method. N isotopic ditution method. prcaution whenquantifying N2 fixation associated with no-nodulatin plants (associative N2 fixation). Assays of nitrogenase activity.
During the past three decades there has been a large amount of research on biological nitrogen fixation, in part stimulated by increasing world prices of nitrogen-containing fertilizers and environmental concerns. In the last several years, research on plant--microbe interactions, and symbiotic and asymbiotic nitrogen fixation has become truly interdisciplinary in nature, stimulated to some degree by the use of modern genetic techniques. These methodologies have allowed us to make detailed analyses of plant and bacterial genes involved in symbiotic processes and to follow the growth and persistence of the root-nodule bacteria and free-living nitrogen-fixing bacteria in soils. Through the efforts of a large number of researchers we now have a better understanding of the ecology of rhizobia, environmental parameters affecting the infection and nodulation process, the nature of specificity, the biochemistry of host plants and microsymbionts, and chemical signalling between symbiotic partners. This volume gives a summary of current research efforts and knowledge in the field of biological nitrogen fixation. Since the research field is diverse in nature, this book presents a collection of papers in the major research area of physiology and metabolism, genetics, evolution, taxonomy, ecology, and international programs.
Biological nitrogen fixation has essential role in N cycle in global ecosystem. Several types of nitrogen fixing bacteria are recognized: the free-living bacteria in soil or water; symbiotic bacteria making root nodules in legumes or non-legumes; associative nitrogen fixing bacteria that resides outside the plant roots and provides fixed nitrogen to the plants; endophytic nitrogen fixing bacteria living in the roots, stems and leaves of plants. In this book there are 11 chapters related to biological nitrogen fixation, regulation of legume-rhizobium symbiosis, and agriculture and ecology of biological nitrogen fixation, including new models for autoregulation of nodulation in legumes, endophytic nitrogen fixation in sugarcane or forest trees, etc. Hopefully, this book will contribute to biological, ecological, and agricultural sciences.
Rhizobia are bacteria which inhabit the roots of plants in the pea family and "fix" atmospheric nitrogen for plant growth. They are thus of enormous economic importance internationally and the subject of intense research interest. Handbook for Rhizobia is a monumental book of practical methods for working with these bacteria and their plant hosts. Topics include the general microbiological properties of rhizobia and their identification, their potential as symbionts, methods for inoculating rhizobia onto plants, and molecular genetics methods for Rhizobium in the laboratory. The book will be invaluable to Rhizobium scientists, soil microbiologists, field and laboratory researchers at agricultural research centers, agronomists, and crop scientists.