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This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant S. aureus. The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Informative and cutting-edge, Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Third Edition is a valuable resource for researchers looking to set up new methods to study S. aureus, and will also be very useful for technicians and scientists working on other bacterial pathogens.
Methicillin-resistant Staphylococcus aureus (MRSA) emerged as a clinically relevant human pathogen more than five decades ago. The virulent bacterium was first detected in hospitals and other health care facilities where vulnerable hosts, frequent exposure to the selective pressure of intensive antimicrobial therapy, and the necessity for invasive procedures created a favorable environment for dissemination. MRSA emerged as an important cause of healthcare-associated infections, particularly central line-associated bloodstream infection, ventilator-associated pneumonia, and surgical site infection (SSI). Despite the adoption of infection-control measures, the incidence of MRSA infection at most U.S. hospitals steadily increased for many years, but it is now decreasing. While the decrease in the incidence of MRSA infection may be due to efforts to screen for MRSA carriage, it may also be due to secular trends (such as efforts to improve patient safety) and to confounders (such as efforts to improve the appropriate use of antibiotics and to decrease healthcare-associated infections in general, including catheter-associated bloodstream infection, ventilator-associated pneumonia, and SSI). A number of analyses suggest that MRSA infections are associated with increased mortality and cost of care when compared with those due to strains that are susceptible to methicillin. Even the availability of newer pharmaceutical agents with specific activity against MRSA has not ameliorated the challenge of caring for patients with MRSA. The widespread use of these agents has been limited, in part due to toxicity, cost, and uncertainty as to optimal indications. The management and control of MRSA have been further complicated by dramatic changes in the epidemiology of transmission and infection observed over the past two decades. Specifically, S. aureus strains resistant to methicillin, once exclusively linked to hospital care, have increasingly been detected among patients in the community who lack conventional risk factors for MRSA infection. Community-acquired MRSA has been linked to outbreaks of infection in hospitals and health care facilities. Conventional strategies for the control of MRSA have focused on the prevention of spread from patient to patient. The effectiveness of hand hygiene in preventing the spread of MRSA has been demonstrated in observational studies in which hand hygiene promotion campaigns were associated with subsequent reductions in the incidence of MRSA among hospitalized patients. While hand hygiene remains important in the effort to control MRSA transmission, the continued spread of the pathogen after its initial introduction in most facilities has prompted efforts to identify additional strategies. The use of contact isolation-including the donning of gowns and gloves when interacting with patients colonized or infected with MRSA and the assignment of such patients to single rooms or to a room with a group of affected patients-has been widely promoted and adopted. Such isolation precautions now are the centerpiece of most authoritative guidelines for MRSA control. Despite the broad consensus associated with the use of contact isolation for MRSA prevention, the specific evidence in support of this practice remains limited and indirect. The objective of this review was to synthesize comparative studies that examined the benefits or harms of screening for MRSA carriage in the inpatient or outpatient settings. The review examined MRSA-screening strategies applied to all hospitalized or ambulatory patients, as well as screening strategies applied to selected inpatient or outpatient populations, and compared them with no screening or with screening of selected patient populations. The review evaluated MRSA-screening strategies that included screening with or without isolation and with or without attempted eradication/decolonization.
Staphylococcus was first recognized as a human pathogen in 1880 and was named for its grape cluster-like appearance. In 1884, Staphylococcus aureus was identified and named for its vibrant golden color, which was later found to be the result of golden toxin production. Here, experts examine in-depth patterns of S. aureus colonization and exposures in humans, mammals, and birds that have led to the development of various clinical diseases. The mode of transmission of S. aureus and different methods for its detection in different samples are defined. Conventional antibiotic options to treat this aggressive, multifaceted, and readily adaptable pathogen are becoming limited. Alternative, novel chemotherapeutics to target S. aureus are discussed in the pages within, including herbal medicines, bee products, and modes of delivery.
This book offers comprehensive information on modern approaches to vulvar lesions, taking into account recent management recommendations and employing the consensus terminology of the International Society for the Study of Vulvovaginal Disease (ISSVD). Further, it breaks with past misconceptions and myths, and explains in detail the modern approach. The aim is to help clinicians perform the differential diagnosis of vulvar conditions and implement the new recommended treatments. The core chapters of the book are arranged in accordance with lesion presentation: red lesions, white lesions, patches and plaques, papules and nodules, erosions and ulcers, blisters, etc. For each lesion, the multidisciplinary management is fully explained, including clarifications of the role of gynecologists, dermatologists, physical therapists, pain specialists, sex therapists, and others. Clinical case presentations and numerous illustrations of treatment procedures are included, supplemented by informative online videos. A separate chapter is devoted to vulvar pain, which is increasingly becoming recognized as a growing problem. Here, again the past approaches are replaced with new paradigms. All of the authors are acknowledged experts in the field and the editor is a past president of the ISSVD. The book will be of value for all vulvar specialists and a wide range of other clinicians.
This book provides a survey of recent advances in the development of antibiofilm agents for clinical and environmental applications. The fact that microbes exist in structured communities called biofilms has slowly become accepted within the medical community. We now know that over 80% of all infectious diseases are biofilm-related; however, significant challenges still lie in our ability to diagnose and treat these extremely recalcitrant infections. Written by experts from around the globe, this book offers a valuable resource for medical professionals seeking to treat biofilm-related disease, academic and industry researchers interested in drug discovery and instructors who teach courses on microbial pathogenesis and medical microbiology.
Diagnostics and Therapy in Veterinary Dermatology presents thorough coverage of the latest discoveries, drugs, and treatments for dermatologic conditions in animals. Chapters written by experts in each respective area of veterinary dermatology contain up-to-date information on new diagnostic tools and tests, autoimmune diseases, parasitic and fungal infections, medical management of acute and chronic conditions, alternative dermatologic therapies, and more. Offering practical solutions for both specialist and general practice veterinarians dealing with dermatology cases, this wide-ranging resource also addresses antibiotic resistance and misuse, the availability of foods for elimination diet trials, problems with generic drugs, emerging infectious diseases, and other important problems currently facing the profession. Throughout the text, veterinary practitioners are provided with real-world guidance on improving how they work up their dermatology cases and strengthening communication between the primary care veterinarian and the dermatologist. Edited by a leading board-certified dermatologist, this volume: Focuses on cats and dogs Includes numerous high-quality clinical photographs illustrating all key concepts Covers topics such as how to use your nursing staff to the fullest, the One Health movement, and how changing climate is increasing the spread of certain dermatologic diseases Discusses approaches for building a better working relationship between clients, primary care veterinarians and dermatologists Provides insights on the future of technology in the diagnosis and treatment of dermatologic diseases Covering the very latest developments in the field, Diagnostics and Therapy in Veterinary Dermatology is essential reading for veterinary dermatologists, veterinary students, and any veterinary general practitioner with a dermatology caseload.
These guidelines provide recommendations that outline the critical aspects of infection prevention and control. The recommendations were developed using the best available evidence and consensus methods by the Infection Control Steering Committee. They have been prioritised as key areas to prevent and control infection in a healthcare facility. It is recognised that the level of risk may differ according to the different types of facility and therefore some recommendations should be justified by risk assessment. When implementing these recommendations all healthcare facilities need to consider the risk of transmission of infection and implement according to their specific setting and circumstances.
Like sharks, epidemic diseases always lurk just beneath the surface. This fast-paced history of their effect on mankind prompts questions about the limits of scientific knowledge, the dangers of medical hubris, and how we should prepare as epidemics become ever more frequent. Ever since the 1918 Spanish influenza pandemic, scientists have dreamed of preventing catastrophic outbreaks of infectious disease. Yet, despite a century of medical progress, viral and bacterial disasters continue to take us by surprise, inciting panic and dominating news cycles. From the Spanish flu and the 1924 outbreak of pneumonic plague in Los Angeles to the 1930 'parrot fever' pandemic and the more recent SARS, Ebola, and Zika epidemics, the last 100 years have been marked by a succession of unanticipated pandemic alarms. Like man-eating sharks, predatory pathogens are always present in nature, waiting to strike; when one is seemingly vanquished, others appear in its place. These pandemics remind us of the limits of scientific knowledge, as well as the role that human behaviour and technologies play in the emergence and spread of microbial diseases.
The first Infection control guidelines for long-term care facilities were published in 1986. Since that time the interest in, and knowledge of, the requirements of infection control programs for long-term care facilities has steadily increased. This document presents the second version and looks at the following points: organizational structure of an infection control program; environmental concerns; departments and services; management of specific care situations; occupational health; and, epidemic investigation and control.