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Ion -Selective Electrodes for Biological Systems provides a user-friendly and practical guide to the manufacture and use of ion-selective electrodes for a wide variety of experimental systems used in biology. The book is aimed at researchers with little practical experience in the field and will direct them through the steps involved in making electrodes, interpretation of the data, requirements of the recording apparatus, potential pitfalls involved in their use, and the range of situations in which they can be used. This is the first book that deals comprehensively with such practical details and will enable the reader to become competent in the use of ion-selective electrodes.
Ion -Selective Electrodes for Biological Systems provides a user-friendly and practical guide to the manufacture and use of ion-selective electrodes for a wide variety of experimental systems used in biology. The book is aimed at researchers with little practical experience in the field and will direct them through the steps involved in making elec
The critically acclaimed guide to the principles, techniques, and instruments of electroanalytical chemistry-now expanded and revised Joseph Wang, internationally renowned authority on electroanalytical techniques, thoroughly revises his acclaimed book to reflect the rapid growth the field has experienced in recent years. He substantially expands the theoretical discussion while providing comprehensive coverage of the latest advances through late 1999, introducing such exciting new topics as self-assembled monolayers, DNA biosensors, lab-on-a-chip, detection for capillary electrophoresis, single molecule detection, and sol-gel surface modification. Along with numerous references from the current literature and new worked-out examples, Analytical Electrochemistry, Second Edition offers clear, reader-friendly explanations of the fundamental principles of electrochemical processes as well as important insight into the potential of electroanalysis for problem solving in a wide range of fields, from clinical diagnostics to environmental science. Key topics include: The basics of electrode reactions and the structure of the interfacial region Tools for elucidating electrode reactions and high-resolution surface characterization An overview of finite-current controlled potential techniques Electrochemical instrumentation and electrode materials Principles of potentiometric measurements and ion-selective electrodes Chemical sensors, including biosensors, gas sensors, solid-state devices, and sensor arrays
We continue in this second volume the plan evident in the first; i.e., of presenting a number of well-rounded up-to-date reviews of important developments in the exciting field of ion-selective electrodes in analytical chemistry. In this volume, in addition to the exciting applications of ISE'S to biochemistry systems represented by the description of enzyme electrodes, there is featured the most recent development in ISE'S, namely, the joining of the electrochemical and solid state expertise, resulting in CHEMFETS. The scholarly survey of the current status of ISE'S will undoubtedly be welcomed by all workers in the field. Tucson, Arizona Henry Freiser vii Contents Chapter 1 Potentiometric Enzyme Methods Robert K. Kobos 1. Introduction . . . . . . 1 2. Soluble Enzyme Systems . . . 5 2.1. Substrate Determinations 5 2.2. Enzyme Determinations . 13 2.3. Inhibitor Determinations. 18 3. Immobilized Enzyme Systems . 19 3.1. Methods of Immobilization. 19 3.2. Characteristics of Immobilized Enzymes 23 3.3. Analytical Applications with Ion-Selective Electrodes 23 4. Enzyme Electrodes 31 4.1. Urea Electrodes 35 4.2. Amygdalin Electrodes 39 4.3. Glucose Electrodes . 40 4.4. Penicillin Electrodes 40 4.5. Amino Acid Electrodes 41 4.6. Nucleotide Electrodes 46 4.7. Uric Acid Electrode 47 4.8. Creatinine Electrode 48 48 4.9. Acetylcholine Electrodes. 4.10. D-Gluconate Electrode 49 4.11. Lactate Electrode 49 4.12. Inhibitor Determination 50 4.13. Substrate Electrodes 50 4.14. Current Trends . . . .
This book broadly reviews the modem techniques and significant applications of chemical sensors and biosensors. Chapters are written by experts in the field – including Professor Joseph Wang, the most cited scientist in the world and renowned expert on sensor science who is also co-editor. Each chapter provides technical details beyond the level found in typical journal articles, and explores the application of chemical sensors and biosensors to a significant problem in biomedical science, also providing a prospectus for the future.This book compiles the expert knowledge of many specialists in the construction and use of chemical sensors and biosensors including nitric oxide sensors, glucose sensors, DNA sensors, hydrogen sulfide sensors, oxygen sensors, superoxide sensors, immuno sensors, lab on chip, implatable microsensors, et al. Emphasis is laid on practical problems, ranging from chemical application to biomedical monitoring and from in vitro to in vivo, from single cell to animal to human measurement. This provides the unique opportunity of exchanging and combining the expertise of otherwise apparently unrelated disciplines of chemistry, biological engineering, and electronic engineering, medical, physiological. - Provides user-oriented guidelines for the proper choice and application of new chemical sensors and biosensors - Details new methodological advancements related to and correlated with the measurement of interested species in biomedical samples - Contains many case studies to illustrate the range of application and importance of the chemical sensors and biosensors
The Principles of Ion Selective Electrodes and of Membrane Transport is a collection of research works on the theory, principles, and fundamentals of ion-selective electrodes and of membrane transport. This book is organized into two parts encompassing 15 chapters that highlight the application of the membrane model. Part A is a general discussion of membrane potentials and membrane transport. This part describes the formulations of the interfacial potential contribution due to phase boundaries. This part also explores the diffusion potential, the nonideality of diffusion layers or membrane phases, the liquid-junction potential arising in conventional potentiometric measuring cells. Other topics covered in this part include the practical solution for the membrane potential; the ion-transport and the electrical properties of bulk membranes; and the characteristics of lipid bilayer membranes. Part B considers the fundamentals of ion-selective electrodes. This part begins with discussions of the principles, response behavior, ion selectivity, and detection limits of solid-state membrane electrodes. This part also examines several important extensions and modifications of the Sandblom-Eisenman-Walker theory; the characteristics of neutral carrier membrane electrodes; and the theory of glass electrodes.