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"There is a growing demand for investigation of biofilm mechanisms in detail for the purpose of controlling diseases as well as for harnessing their potential for industrial biotechnology applications. Although biofilms have greater roles in disease biology and offer better yields than suspended cells in bioprocesses, little emphasis has placed on biofilms in several universities' curricula. This book aims to provide a basic understanding about biofilm formation, factors influencing biofilm formations, and engineering strategies for improving or inhibiting the growth of biofilms. This book will serve as course material for the undergraduate students, graduate students, and faculty who are interested in offering a course on biofilm engineering. This book covers techniques that are relevant to biofilm characterization, which will be helpful for the researchers to gain basic understanding about the subject"--
This book details the widely accepted hypothesis that the majority of bacteria in virtually all ecosystems grow in matrix-enclosed biofilms. The author, who first proposed this biofilm hypothesis, uses direct evidence from microscopy and from molecular techniques, arguing cogently for moving beyond conventional culture methods that dominated microbiology in the last century. Bacteria grow predominantly in biofilms in natural, engineered, and pathogenic ecosystems; this book provides a solid basis for the understanding of bacterial processes in environmental, industrial, agricultural, dental and medical microbiology. Using a unique "ecological" perspective, the author explores the commensal and pathogenic colonization of human organ systems.
The six years that have passed since the publication of the first edition have brought significant advances in both biofilm research and biofilm engineering, which have matured to the extent that biofilm-based technologies are now being designed and implemented. As a result, many chapters have been updated and expanded with the addition of sections reflecting changes in the status quo in biofilm research and engineering. Emphasizing process analysis, engineering systems, biofilm applications, and mathematical modeling, Fundamentals of Biofilm Research, Second Edition provides the tools to unify and advance biofilm research as a whole. Retaining the goals of the first edition, this second edition serves as: A compendium of knowledge about biofilms and biofilm processes A set of instructions for designing and conducting biofilm experiments A set of instructions for making and using various tools useful in biofilm research A set of computational procedures useful in interpreting results of biofilm research A set of instructions for using the model of stratified biofilms for data interpretation, analysis, and biofilm activity prediction
This book reviews the current concepts in biofilm formation and its implications in human health and disease. The initial chapters introduce the mechanisms of biofilm formation and its composition. Subsequently, the chapters discuss the role of biofilm in acute and chronic infections. It also explores the pivotal role of both innate and adaptive immunity on the course of biofilm infection. In addition, the book elucidates the bacterial biofilm formation on implantable devices and the current approaches to its treatment and prevention. It analyzes the possible relationship between antimicrobial resistance and biofilm formation. Finally, the book also summarizes the current state-of-the-art therapeutic approaches for preventing and treating biofilms. This book is a useful resource for researchers in the field of microbiology, clinical microbiology, and also medical practitioners.
This book serves as a manual of research techniques for electrochemically active biofilm research. Using examples from real biofilm research to illustrate the techniques used for electrochemically active biofilms, this book is of most use to researchers and educators studying microbial fuel cell and bioelectrochemical systems. The book emphasizes the theoretical principles of bioelectrochemistry, experimental procedures and tools useful in quantifying electron transfer processes in biofilms, and mathematical modeling of electron transfer in biofilms. It is divided into three sections: Biofilms: Microbiology and microbioelectrochemistry - Focuses on the microbiologic aspect of electrochemically active biofilms and details the key points of biofilm preparation and electrochemical measurement Electrochemical techniques to study electron transfer processes - Focuses on electrochemical characterization and data interpretation, highlighting key factors in the experimental procedures that affect reproducibility Applications - Focuses on applications of electrochemically active biofilms and development of custom tools to study electrochemically active biofilms. Chapters detail how to build the reactors for applications and measure parameters
In the context of wastewater treatment, Bioelectrochemical Systems (BESs) have gained considerable interest in the past few years, and several BES processes are on the brink of application to this area. This book, written by a large number of world experts in the different sub-topics, describes the different aspects and processes relevant to their development. Bioelectrochemical Systems (BESs) use micro-organisms to catalyze an oxidation and/or reduction reaction at an anodic and cathodic electrode respectively. Briefly, at an anode oxidation of organic and inorganic electron donors can occur. Prime examples of such electron donors are waste organics and sulfides. At the cathode, an electron acceptor such as oxygen or nitrate can be reduced. The anode and the cathode are connected through an electrical circuit. If electrical power is harvested from this circuit, the system is called a Microbial Fuel Cell; if electrical power is invested, the system is called a Microbial Electrolysis Cell. The overall framework of bio-energy and bio-fuels is discussed. A number of chapters discuss the basics – microbiology, microbial ecology, electrochemistry, technology and materials development. The book continues by highlighting the plurality of processes based on BES technology already in existence, going from wastewater based reactors to sediment based bio-batteries. The integration of BESs into existing water or process lines is discussed. Finally, an outlook is provided of how BES will fit within the emerging biorefinery area.
The book provides the readers of various discipline an easy understanding of the latest biophysical techniques pertaining to microbiology. Biofilm associated chronic infection is a major health problem and a serious concern to doctors, scientists and other health workers as it develops antibiotic and multi-drug resistance. This book describes various protocols utilized in the detection of the biofilm. The book has been divided into six sub sections which provides pertinent information about the various biophysical techniques and instruments that are used for detecting and analyzing the biofilm formation upon biotic and abiotic surfaces. The readers will be able to identify the techniques that can best cater information to solve the problem at hand. This book attempts to compile the latest information on the recent advances in the various functional aspects of microbial biofilms, their pathogenesis, present day treatments as well as detection strategies. This book is meant for researchers in the field of microbiology and interested in understanding microbial pathogenesis, quorum sensing and biofilm formation.
Biofilms -- Science and Technology covers the main topics of biofilm formation and activity, from basic science to applied aspects in engineering and medicine. The book presents a masterly discussion of microbial adhesion, the metabolism of microorganisms in biofilms, modelling of mass transfer and biological reaction within biofilms, as well as the behaviour of these microbial communities in industry (waste water treatment, heat exchanger biofouling, membranes, food processing) and in medicine (teeth, implants, prosthetic devices). Laboratory techniques and industrial monitoring methods are also presented. The book is directed at readers at the postgraduate level and is organised as a textbook, containing 11 chapters, a glossary, and a detailed subject index.
The study of biofilm considers the close association of micro-organisms with each other at interfaces and is relevant to a variety of disciplines, including medicine, dentistry, bioremediation, biofouling, water technology, engineering and food science. Although the habitats studied differ widely, some common elements exist such as method of attachment, coadhesion and regulation of biofilm phenotype and architecture. This book aims to distil the common principles of biofilm physiology and growth for all interested disciplines.
The latest Methods for Wastewater Treatment Using Fixed-Film Processes This Water Environment Federation resource provides complete coverage of pure fixed-film and hybrid treatment systems, along with details on their design, performance, and operational issues. Biofilm Reactors discusses factors that affect the design of the various processes, appropriate design criteria and procedures, modeling techniques, equipment requirements, and construction methods. Operational issues associated with each type of process are presented, including potential problems and corrective actions. Real-world case studies illustrate the application of the technologies presented in this authoritative volume. Biofilm Reactors covers: Biology of fixed-film processes Trickling filter and combined trickling filter suspended-growth process design and operation Rotating biological contactors Moving-bed biofilm reactors Hybrid processes Biological filters New and emerging fixed-film technologies Clarification Effluent filtration Development and application of models for integrated fixed-film activated sludge, moving-bed reactors, biological aerated filters, and trickling filters