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During the last few years, immunoassay has gained tremendous popularity in clinical and research laboratories and has been applied to determine hormone, enzyme, protein, drug, and infectious agents. The aim of this book is to provide clinical laboratory personnel and students with an understanding of the principle of immunoassay and the production of reagents for immunoassay.
Each chapter of this book aims to explore the basic physical and chemical principles involved in the immunoassay techniques discussed. The book also looks at the optimization and limitations of methodology and concludes with a brief overview of the application of the performance of the technology.
First multi-year cumulation covers six years: 1965-70.
Advances in Clinical Chemistry
Containing updated and new information on advanced technology - including micro and nanoscale immunoassays - this text provides a mix of practical information coupled with a review of clincal applications and practical examples.
Thirty years have elapsed since the first description by S. A. BERSON and R. S. Y ALOW of the basic principles of radioimmunoassay (RIA). During this period of time, RIA methodology has been instrumental to the growth of many areas of biomedical research, including endocrinology, oncology, hematology, and pharmacology. It has done so by providing a relatively simple universal tool allowing, for the first time, the detection of endogenous mediators that are present 12 10 in body fluids at concentrations as low as 10- _10- M. The fundamental nature of this discovery and the wide-ranging fall-out of basic and clinical knowledge derived from its application have been acknowledged by the many honors tributed to its pioneers, including the Nobel Prize awarded to Dr. Y ALOW 10 years ago. Although several excellent books have been published during the past decades covering various aspects of RIA methodology, we felt the need, as pharmacologists, for a comprehensive discussion of the methodological and conceptual issues related to the main classes of mediators of drug action and to drugs themselves. Thus, we gladly accepted the challenge provided by the invitation to edit a volume of the Handbook of Experimental Pharmacology on Radioimmunoassay in Basic and Clinical Pharmacology. We tried to balance the emphasis placed on more general aspects of the RIA methodology and that on specific mediators.
Rapid Detection and Identification of Infectious Agents is a collection of papers presented at the International Symposium on Rapid Detection and Identification of Infectious Agents held on October 5-7, 1983, in Oakland, California, and organized by the Naval Biosciences Laboratory of the School of Public Health of the University of California at Berkeley. Contributors examine progress in the field of rapid diagnosis of infectious diseases, with a particular emphasis on DNA probe-based assays and monoclonal and polyclonal antibody-based immunoassays. This volume is organized into five sections encompassing 20 chapters. It begins with an overview of state-of-the-art methods for rapid detection and identification of infectious agents, including technology that is currently applied in clinical microbiology, as well as concerns regarding the political and scientific climates, which have an impact on health care and clinical microbiology. Chapters are organized to deal with a single diagnostic type of test for a given broad group of organisms. The approach is to compare the strengths and weaknesses of each of the new diagnostic procedures, using the same type of clinical material whenever possible. The book gives consideration to the fundamental design of DNA probes and probe assay systems, the clinical comparison of immunologic assays for the diagnosis of meningococcal disease, and immunodiagnostics for viral and parasitic pathogens. This book will be of value to scientists and researchers interested in immunology and infectious diseases, as well as the methods used to detect and identify them.