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In this third edition, more than 40 renowned authorities introduce and update chapters on the theory, fundamentals, techniques, and instrumentation of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC), highlighting the latest procedures and applications of TLC to 19 important compound classes and coverage of TLC applications by compound type. Easily adaptable to industrial scenarios , the Handbook of Thin-Layer Chromatography, Third Edition supports practical research strategies with extensive tables of data, offers numerous figures that illustrate techniques and chromatograms, and includes a glossary as well as a directory of equipment suppliers.
This book gives a practical introduction to one of the more popular separation techniques. Readers will learn to perform separations and will develop the ability to make an educated guess as to what the conditions will be to separate a new mixture of compounds. The authors provide classes of compound and background theory that quickly develop the skills of the student learning thin layer chromatography. Chapter coverage includes stationary phase, mobile phase, practical techniques, applications, recent developments, and advantages and disadvantages of thin layer chromatography. It also includes a bibliography of texts providing additional separations for further study.· Stationary Phase· Mobile Phase· Sample· Practical Techniques· Applications· Recent Developments· Advantages and Disadvantages of Tlc· Self Assessment Questions and Responses · Units of Measurement
Thin-Layer Chromatography (TLC) is a modern, reliable tool that complements other chromatographic techniques. This book provides a practical guide to the basic principles, procedures and pitfalls on the practical application of TLC. Thin Layer Chromatography: A Modern Practical Approach offers a sequence of chapters following the steps of the technique as the chromatographer would follow them. The chapters provide a choice of sorbent best suited to the separation intended, followed by pre-treatment required for the sample, applying the sample to the sorbent layer, development procedure, visualisation and detection, and finally quantification. Imaging and hyphenation techniques are described. The reasons why recommendations are made for specific and more general methods are covered. The book also provides an overview of some recent developments in the field.
The present edited book is the presentation of 18 in-depth national and international contributions from eminent professors, scientists and instrumental chemists from educational institutes, research organizations and industries providing their views on their experience, handling, observation and research outputs on HPTLC, a multi-dimensional instrumentation. The book describes the recent advancements made on TLC which have revolutionized and transformed it into a modern instrumental technique HPTLC. The book addresses different chapters on HPTLC fundamentals: principle, theory, understanding; instrumentation: implementation, optimization, validation, automation and qualitative and quantitative analysis; applications: phytochemical analysis, biomedical analysis, herbal drug quantification, analytical analysis, finger print analysis and potential for hyphenation: HPTLC future to combinatorial approach, HPTLC-MS, HPTLC-FTIR and HPTLC-Scanning Diode Laser. The chapters in the book have been designed in such away that the reader follows each step of the HPTLC in logical order.
Computer Forensics: Evidence Collection and Management examines cyber-crime, E-commerce, and Internet activities that could be used to exploit the Internet, computers, and electronic devices. The book focuses on the numerous vulnerabilities and threats that are inherent on the Internet and networking environments and presents techniques and suggestions for corporate security personnel, investigators, and forensic examiners to successfully identify, retrieve, and protect valuable forensic evidence for litigation and prosecution. The book is divided into two major parts for easy reference. The first part explores various crimes, laws, policies, forensic tools, and the information needed to understand the underlying concepts of computer forensic investigations. The second part presents information relating to crime scene investigations and management, disk and file structure, laboratory construction and functions, and legal testimony. Separate chapters focus on investigations involving computer systems, e-mail, and wireless devices. Presenting information patterned after technical, legal, and managerial classes held by computer forensic professionals from Cyber Crime Summits held at Kennesaw State University in 2005 and 2006, this book is an invaluable resource for thosewho want to be both efficient and effective when conducting an investigation.
Plant Drug Analysis has proven an invaluable and unique aid for all those involved with drug production and analysis, including pharmacists, chemical and pharmaceutical researchers and technicians, drug importers and exporters, governmental chemical control agencies, and health authorities. From the reviews of the German Edition: "The reviewer would like to recommend this excellent book to all chromatographers, as he considers it highly relevant to the solution of numerous problems. Its main purpose is the demonstration of thin-layer chromatograms of the usual commercial drugs as an aid in testing for identity and purity. ... 165 colour plates, each showing 6 chromatograms and all of superb quality photographs ..." (Journal of Chromatography)
In the study and conservation of art and artifacts, natural organic materials are frequently encountered in components such as coatings, binders, and adhesives. The identification of these materials is often crucial to the attempt to characterize the technologies employed by artists or craftspeople, understand the processes and causes of deterioration, and plan appropriate conservation treatments. Yet the limited resources of many conservation laboratories put many analysis techniques beyond their reach. Thin-layer chromatography can help fill this gap. The volume consists of a handbook, protocols, and guide to reference materials. The handbook serves as a primer for the basic application of thin-layer chromatography to the analysis of binding media, adhesives, and coatings found on cultural objects; the protocols provide step-by-step instructions for the laboratory procedures involved in typical analyses; and the guide to reference materials aids in the understanding of the types of materials and documentation needed for accurate analyses by thin-layer chromatography.
Separation Methods in Drug Synthesis and Purification
Thin-layer chromatography (TLC) is a powerful, fast and inexpensive analytical method. It has proven its usefulness in pharmaceutical, food and environmental analysis. This new edition of the practical TLC guide features a completely revised chapter on documentation, now including the use of digital cameras. Selected new sorbents and instruments are also introduced. Why has the prior edition been successful? All steps of the analytical procedure are clearly explained, starting with the choice of a suitable TLC technique and ending with data evaluation and documentation. Special emphasis is put on the proper choice of materials for TLC. Properties and functions of various materials and the TLC equipment are described, covering e. g. precoated layers, solvents and developing chambers, including information on suppliers. Many practical hints for trouble shooting are given. All this is illustrated with numerous coloured figures. How to use TLC in compliance with GLP/GMP regulations is described in detail, including the required documentation. Therefore the reader can very easily compile his own standard operating procedures.