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Erika, an eighteen-year-old Gamma with superior fighting skills, finds herself being the mate of one of the strongest Alphas that has ever existed, who is also a control freak. Erika is asked to submit, but all she wants is to be free. It would be a long and violent journey to change from a Gamma to a Luna.
Erika, an eighteen-year-old Gamma with superior fighting skills, finds herself being the mate of one of the strongest Alphas that has ever existed, who is also a control freak. Erika is asked to submit, but all she wants is to be free. It would be a long and violent journey to change from a Gamma to a Luna.
A bewitching memoir about the lures, torments, and rewards of making and performing music in the indie rock world Dean Wareham's seminal bands Galaxie 500 and Luna have long been adored by a devoted cult following and extolled by rock critics. Now he brings us the blunt, heartbreaking, and wickedly charismatic account of his personal journey through the music world-the artistry and the hustle, the effortless success and the high living, as well as the bitter pills and self-inflicted wounds. It captures, unsparingly, what has happened to the entire ecosystem of popular music over a time of radical change, when categories such as "indie" and "alternative" meant nothing to those creating the music, but everything to the major labels willing to pay for it. Black Postcards is a must-have for Wareham's many fans, anyone who has ever been in a band, or the listeners who have taken an interest in the indie rock scene over the last twenty years.
Type IV secretion systems (T4SSs) are highly versatile membrane-associated transporter machines used by Gram-negative and Gram-positive bacteria to deliver substrate molecules to a large variety of target cells. This volume summarizes our current knowledge of the large variety and structural diversity of T4SSs in pathogenic Escherichia, Agrobacterium, Legionella, Coxiella, Bartonella, Helicobacter, Enterococcus and other species. Divided into 13 chapters contributed by leading experts, it presents findings that significantly enhance our understanding of how various pathogens manipulate host cell functions to trigger bacterial uptake, promote intracellular growth, suppress defense mechanisms and of how bacteria spread antibiotic resistances, thus facilitating bacterial colonization and disease development. The book is an invaluable source of information for researchers and clinicians.
This book focuses on the mechanical properties of cells, discussing the basic concepts and processes in the fields of immunology, biology, and biochemistry. It introduces and explains state-of-the-art biophysical methods and examines the role of mechanical properties in the cell/protein interaction with the connective tissue microenvironment. The book presents a unique perspective on cellular mechanics and biophysics by combining the mechanical, biological, physical, biochemical, medical, and immunological views, highlighting the importance of the mechanical properties of cells and biophysical measurement methods. The book guides readers through the complex and growing field of cellular mechanics and biophysics, connecting and discussing research findings from different fields such as biology, cell biology, immunology, physics, and medicine. Featuring suggestions for further reading throughout and addressing a wide selection of biophysical topics, this book is an indispensable guide for graduate and advanced undergraduate students in the fields of cellular mechanics and biophysics.
Recent studies show that changes in Glutamate/GABA synapses and related pathways may determine whether the synaptic plasticity that occurs as a response to stress is adaptive or maladaptive. Findings have shed new light on the mechanisms that determine the effects of stress on cognitive and affective function. Researchers have brought a wide range of techniques to bear on the study of this problem, including cutting-edge live imaging techniques, electrophysiology, glutamate release from isolated live synaptic terminals, development of transgenic and animal models and new behavioral methods. This book provides an overview of these recent findings and of the techniques used as well as a discussion of how the molecular, cellular, and functional effects of stress may trigger or precipitate neuropsychiatric disorders such as depression, schizophrenia, anxiety and PTSD.
This comprehensive guide is an ideal reference work for film specialists and enthusiasts. First published in 1984 but continuously updated ever since, CineGraph is the most authoritative and comprehensive encyclopedia on German-speaking cinema in the German language. This condensed and substantially revised English-language edition makes this important resource available to students and researchers for the first time outside its German context. It offers a representative historical overview through bio-filmographical entries on the main protagonists, from the beginnings to the present day. Included are directors and actors, writers and cameramen, composers and production designers, film theorists and critics, producers and distributors, inventors and manufacturers. An appendix includes short introductory essays on specific periods and movements, such as Early Film, Weimar, Nazi Cinema, DEFA, New German Cinema, and German film since unification, as well as on cinematic developments in Austria and Switzerland. Sections that crossreference names around specific professional groups and themes will prove equally invaluable to researchers.
Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and back. In Microtubule Dynamics: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics in vitro and in cells. Beginning with the question of how to analyze microtubule dynamics, the volume continues with detailed descriptions of how to isolate tubulin from different sources and with different posttranslational modifications, methods used to study microtubule dynamics and microtubule interactions in vitro, techniques to investigate the ultrastructure of microtubules and associated proteins, assays to study microtubule nucleation, turnover, and force production in cells, as well as approaches to isolate novel microtubule-associated proteins and their interacting proteins. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Definitive and practical, Microtubule Dynamics: Methods and Protocols provides the key protocols needed by novices and experts on how to perform a broad range of well-established and newly-emerging techniques in this vital field.
CMJ New Music Report is the primary source for exclusive charts of non-commercial and college radio airplay and independent and trend-forward retail sales. CMJ's trade publication, compiles playlists for college and non-commercial stations; often a prelude to larger success.