Published: 1991-01
Total Pages: 333
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Parts A and B, companion volumes on fluorescence microscopy, are sourcebooks of methods for living cells. Part A comprehensively covers methodology: fluorescent analogs, procedures for fluorescent labeling of various cellular structures, and fluorescence microscopy. Part B describes instrumentation and quantitation analysis for imaging and spectroscopy. Key Features * Convenient bench-top format Comprehensive methods inluded for: * Tracing proteins, peptides, hormones, and toxins * Labeling cell surfaces, mitochondria, endoplasmic reticulum, and endocytic compartments * Preparation of fluorescent probes and their incorporation into cells * Culturing cells under the microscope * Basic fluorescence microscopy and imaging techniques