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The formation of atmospheric nitrogen gas by denitrifying bacteria may represent a significant nutrient sink in natural ecosystems. The rate of denitrification has often been difficult to measure in situ, however, and new methodologies should stimulate research on distribution of activity in space and time. The load of fertilizer nitrogen in modem agriculture has led to increasing nutrient reservoirs in recipient subsoils, aquifers, inland waters and coastal seas. By its conversion of nitrate to atmospheric nitrogen, bacterial denitrification is the only biological process to potentially reduce the impact of increasing nutrient loadings by fertilizer nitrogen in the environment. As part of a scientific program set up by the Danish Ministry of Environment to study environment cycling of nitrogen, phosphorous and organic matter (NPO program) in the light of agricultural, domestic and industrial activities, a symposium on DENITRIFICATION IN SOIL AND SEDIMENT was held at the University of Aarhus, Denmark from 6-9 June 19i\9. On the basis of lectures given at the symposium, this book contains a number of invited contributions on the regulation of denitrification activity (control of enzyme synthesis and activity) and measurement of in situ rates of denitrification in terrestrial and aquatic environments (control factors, diel and seasonal variations, etc). Emphasis has been placed on including the recent improvements in methodologies and current understanding of process regulation, however the book also contains examples of integrated research on the significance of denitrification in environmental nutrient cycling.
Nitrogen transformation processes; The nitrogen cycle in different systems; Advances in nitrogen methodology; 15N recovery techniques in the field.
Agriculture in the industrial world has gone A common interest of the contributors is increas through dramatic changes over the past decades. ing the understanding of the turnover of carbon Mechanization in combination with high inputs of and inorganic nutrients in terestrial ecosystems. fertilizers and pesticides has turned deficits of agri The authors approach this topic from different cultural products into surplus. Over the same directions depending on their interests and ex period we have experienced increased environment pertise. Difficulties are identified in the quantifica al problems in both the atmosphere and our water tion of below-ground production where death and resources, which have been associated with the re-growth, if incorporated into the calculations, changes in management practices. can change production figures considerably as Concern about the potential pollution by compared to values derived from "peak" estimates. nitrogen fertilizers as well as the low utilization The role of root-derived carbon is investigated in efficiency of applied nitrogen by plants has created relation to nutrient competition between roots and a need for a better understanding of nitrogen microorganisms, the cost of N2 fixation and the cycling in the plant-soil-water system. To achieve decomposition of organic nitrogen. Mycorrhizae this, it is neccessary to study process interactions use root-derived carbon and their roles in phos and process regulation in an ecosystem context. phorus conservation and in supplying nutrients to During the last decade many ecosystem studies the host are exemplified.
Thoroughly updated and revised, this second edition of the bestselling Soil Sampling and Methods of Analysis presents several new chapters in the areas of biological and physical analysis and soil sampling. Reflecting the burgeoning interest in soil ecology, new contributions describe the growing number and assortment of new microbiological
Standardized methods and measurements are crucial for ecological research, particularly in long-term ecological studies where the projects are by nature collaborative and where it can be difficult to distinguish signs of environmental change from the effects of differing methodologies. This second volume in the Long-Term Ecological Research (LTER) Network Series addresses these issues directly by providing a comprehensive standardized set of protocols for measuring soil properties. The goal of the volume is to facilitate cross-site synthesis and evaluation of ecosystem processes. Chapters cover methods for studying physical and chemical properties of soils, soil biological properties, and soil organisms, and they include work from many leaders in the field. The book is the first broadly based compendium of standardized soil measurement methods and will be an invaluable resource for ecologists, agronomists, and soil scientists.
Soil sampling for microbiological analysis; Statistical treatment of microbial data; Soil sterilization; Soil water potencial; Most probable number counts; Light microscopic methods for studying soil microorganisms; Viruses; Recovery and enumeration of viable bacteria; Coliform bacteria; Autotrophic nitrifying bacteria; Free-living dinitrogen-fixing bacteria; Legume nodule symbionts; Anaerobic bacteria and processes; Denitrifiers; Actiomycetes; Frankia and the actinorhizal symbiosis; Filamentous fungi; Vesicular-arbuscular mycorrhizal fungi; Isolation of microorganisms producting antibiotics; Microbiological procedures for biodegradation research; Algae and cyanobacteria; Marking soil bacteria with lacZY; Detection of specific DNA sequences in environmental sample via polymerase chaim reaction; Isolation and purification of bacterial DNA from soil; Microbial biomass; Soil enzymes; Carbon mineralization; Isotopic methods for the study of soil organic matter dynamics ; Practical considerations in the use of nitrogen tracers in agricultural and environmental research; Nitrogen availability; Nitrogen mineralization, immobilization, and nitrification; Dinitrogen fixation; Measuring denitrification in the field; Sulfur oxidation and reduction in soils; Iron and manganese oxidation and reduction.
One of the primary references on analytical methods in soil science, Part 2 of the Methods series will be useful to all biogeoscientists, especially those with an interest in microbiology or bioremediation.
This book contains the papers presented at a Nato Advanced Re search Workshop entitled "DENITRIFICATION IN THE N-CYCLE," held in Braunschweig (W-Germany) from 24 to 27 Mai 1983. All expenses were provided by the North Atlantic Treaty Organization. The scientific programme was in the first instance planned by some members of the Eco-Science Panel under the stimulating organization of Dr. Oscar Ravera and the final programme was prepared in co-operation between Ravera and myself. However, even during the meeting important con tributions were added. The meeting was hosted by the Microbiologi cal Dept. of F.A.L., which also took care of the organizatory as pects. Nitrate is constantly lost from both terrestial and aquatic ecosystems, causing rnixed feelings between ecologists and agricul turists. While bacteriologically very rnuch is known, the ecology of the processes is still poorly understood, nor can it be evaluated what it rneans as an econornic loss for farrners and world food produc tion. Therefore this NATO Advanced Workshop was established to per mit a lirnited nurnber of scientists active in this field to corne to gether for a short while to address the following objectives: 1) To exchange ideas between scientists (bacteriologists and ecologists) and agronornists. 2) To assess the state of the art. 3) To discuss the difficulties of experimentation in the field. 4) To define future research. In order to accornplish these objectives, the workshopwas organ ized in three parts with the following thernes: 1) Bacteriological aspects of dentrification