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"Experimental Plant Virology” provides the updated methodology for studying the genomic characterization and mechanisms of infection, the quantitative determination as well as the diagnosis of plant pathogenic viruses. With illustrations showing viral symptoms and ultra-structures, clear and concise descriptions, the book presents the latest developments in experimental plant virology. This book is intended for researchers, university teaching staff, graduate students and undergraduates in plant science. Dr. Jishuang Chen is a professor of plant pathology at the Institute of Bioengineering, Zhejiang Sci-Tech University, China.
Viruses require a special approach to establish their presence in a diseased plant since they are not visible, even under a light microscope. This manual describes in detail a variety of protocols for determining the properties and identity of a virus and its behavior in infected plants. A Springer Lab Manual.
For the past twenty years I have worked as an applied plant virologist, attempting to identify and control virus diseases in field crops. During the last ten years it has been my privilege to present short courses in plant virology to final-year students studying plant pathology, micro biology and general botany. Throughout the period I have been lecturing, it has been possible to recommend several excellent 'library' books for further reading in plant virology, but there has been no publication covering applied plant virology that a student might consider purchasing. With teaching requirements in mind this book has been written to provide a concise introduction to applied plant virology based on the experiences I have gained working on virus diseases, both in an applied laboratory and in the field. The text concentrates on introducing the reader to aspects of plant virology that would be encountered every day by an applied virologist trying to identify viruses and develop control measures for virus diseases of crop plants. Although a brief introduction to virus structure and its terminology is given in the opening chapter of the book, no attempt is made to cover in detail the more fundamental aspects of virus structure, biochemistry and replication. Similarly, the symptoms caused by individual viruses are not described, although the various types of symptoms that plant viruses cause and which might be encountered by a student or research worker are described.
"Experimental Plant Virology” provides the updated methodology for studying the genomic characterization and mechanisms of infection, the quantitative determination as well as the diagnosis of plant pathogenic viruses. With illustrations showing viral symptoms and ultra-structures, clear and concise descriptions, the book presents the latest developments in experimental plant virology. This book is intended for researchers, university teaching staff, graduate students and undergraduates in plant science. Dr. Jishuang Chen is a professor of plant pathology at the Institute of Bioengineering, Zhejiang Sci-Tech University, China.
Plant Virology, Second Edition, was written to cover the substantial developments in many areas of plant virology since the first edition was published. Advances have been made in all branches of the subject, but these have been most far reaching with respect to the structure of viruses and of their components, and in the understanding of how viral genomes are organized and how viruses replicate in cells. Significant developments have also occurred in the understanding of how viruses are transmitted by invertebrates and in the application of control measures for specific diseases. The taxonomy of viruses has advanced significantly, and there are now 25 internationally approved families and groups of plant viruses. All these developments have required that most sections be entirely rewritten. This book is intended primarily for graduate students in plant pathology, plant virology, general virology, and microbiology, and for teachers and research workers in these fields. It should also prove useful to some people in related disciplines—molecular biologists, biochemists, plant physiologists, and entomologists.
Most books on epidemiology have treated the subject from a statistical, mathematical or computer applicational point of view. However, experiments must be performed first to provide the data for models which in turn can then be proven by further experimentation. This mutual interplay of theory and empirics gives epidemiology its scientific thrust and charm. This book provides a choice of methods for varying applications and objectives, covering all important aspects for the designing of experiments. Furthermore, the reader is supplied with solutions to his experimental problems and many "tricks of the trade". The newcomer to the field will also profit by this methodology guide.
Topics covered in this book include RNA silencing and its suppression in plant virus infection, virus replication mechanisms, the association of cellular membranes with virus replication and movement, plant genetic resistance to viruses, viral cell-to-cell spread, long distance movement in plants, virus induced ER stress, virus diversity and evolution, virus-vector interactions, cross protection, geminiviruses, negative strand RNA viruses, viroids, and the diagnosis of plant viral diseases using next generation sequencing. This book was anticipated to help plant pathologists, scholars, professors, teachers and advanced students in the field with a comprehensive state-of-the-art knowledge of the subject.
Diagnosis of Plant Virus Diseases presents a comprehensive summary of methods currently available for the diagnosis of plant diseases caused by viruses and viroids. Up-to-date literature references are provided, brief accounts of the basis for particular methods are included, and detailed protocols are presented. Procedures discussed include the use of host plants, electron microscopy of in vitro preparations, serological procedures (especially forms of ELISA, monoclonal antibodies, serological specific electron microscopy, and immunoblotting), and nucleic acid hybridization procedures. Strategies are outlined for implicating virus-like pathogens as causes of diseases of unknown etiology, and problems involved in identifying complexes of transmission-dependent and helper viruses are discussed. The book will be extremely useful for phytopathologists, plant virologists, and research students and workers in plant virology laboratories and diagnostic plant pathology laboratories.
All the information you need on plant viruses in a single volume The Handbook of Plant Virology is a comprehensive guide to the terms and expressions commonly used in the study of plant virology, complete with descriptions of plant virus families down to the generic level. Rather than simply listing terms in alphabetical order, this unique book links each term to related terms within a theme and adds commentary from authors whose specific expertise adds additional dimensions to the topics. The result is an invaluable resource for research workers, educators, and students working in plant virology and pathology, crop protection, molecular biology, and plant breeding. The Handbook of Plant Virology provides enough details and background in the discussion of each topic to present a clear and thorough understanding of terms without the lengthy analysis found in most textbooks. The book’s first section covers: the mechanics of virus classification internal and external symptoms (with color illustrations) isolation and purification genome packaging replication and gene expression detection and identification various methods of virus transmission serology forecasting disease development recombination control strategies economic importance and much more The second section of The Handbook of Plant Virology is devoted to concise descriptions of the 81 genera and 18 families of plant viruses, including: positive-sense, single-stranded RNA viruses, such as Potyviridae, Sequiviridae, and Comoviridae double-stranded RNA viruses, such as Reoviridae and Partitiviridae negative-sense, single-stranded RNA viruses, such as Rhabdoviridae and Bunyaviridae single-stranded DNA viruses, such as Geminiviridae, Pseudoviridae, Metaviridae The Handbook of Plant Virology also includes photos, illustrations, figures, diagrams, and brief, but detailed, bibliographies. The book’s concise mix of information on currently assigned taxonomic families and the genera of plant viruses make it an essential reference tool for practitioners, researchers, educators, and students.
This book provides a comprehensive look at the field of plant virus evolution. It is the first book ever published on the topic. Individual chapters, written by experts in the field, cover plant virus ecology, emerging viruses, plant viruses that integrate into the host genome, population biology, evolutionary mechanisms and appropriate methods for analysis. It covers RNA viruses, DNA viruses, pararetroviruses and viroids, and presents a number of thought-provoking ideas.