Download Free Design And Synthesis Of Esipt Based Fluorescent Probes For The Sensing Of Potential Analytes With Cd Copy Book in PDF and EPUB Free Download. You can read online Design And Synthesis Of Esipt Based Fluorescent Probes For The Sensing Of Potential Analytes With Cd Copy and write the review.

Over the past few decades, there has been significant interest in developing fluorescent probes, because they are useful tools for biological studies. As effective analytical techniques, fluorescent probes utilize distinct advantages offered by fluorescence detection in terms of sensitivity, selectivity, and fast response time. When fluorescent probes interact selectively with target molecules, ions or biological specimens, they can generate large optical responses. Since most ions or molecules, such as Zn2+, Ca2+, or pyrophosphate ion (PPi), are non-fluorescent, chemosensors having analyte binding-triggered fluorescence are appealing in many fields, like analytical chemistry, clinical biochemistry, medicine, and environmental science.This dissertation is devoted to the design, synthesis, and characterization of novel fluorescent sensors for Zn2+ and its associated applications. Chapter II of this dissertation presents several novel terpyridine-based fluorescent sensors with different substituents affecting the electronic and steric nature of the terpyridine (tpy) fluorophore. Sensors are designed to establish the correlation between sensor structure and its photophysical properties. Low temperature fluorescence is used to evaluate the essential role of intramolecular charge transfer (ICT) in zinc binding-induced fluorescence changes. The tpy molecular fragment has a relatively large [pi]-conjugated system which enables the potential [pi-pi] interaction between two tpy platforms and affects the fluorescence of tpy ligands. Chapter III introduces a dimeric tpy ligand containing two tpy fragments connected via a meta-phenylene unit. The detailed spectroscopic study shows that this ligand displays an attractive fluorescence turn-on, in sharp contrast to mono(tpy) ligand that shows fluorescence quenching upon binding Zn2+. The result suggests the existence of delicate structural influences on fluorescence of tpy derivatives.Chapter IV is devoted to 2-(2'-hydroxyphenyl)-1,3-benzoxazole (HBO) and 2-(2'-hydroxyphenyl)-1,3-benzothiazole (HBT) derivatives featured with a structural potential of excited-state intramolecular proton transfer (ESIPT). The study reveals additional information on the binding of HBO or HBT to metal cations, which aids the sensor design for Zn2+ and PPi detection. The molecular design aims to realize ESIPT process control upon complexation with an analyte. Chapter V is devoted to the synthesis of bis(HBO) derivatives which bind Zn2+ selectively and emit near-infrared (NIR) fluorescence as a consequence of metal ion binding-induced ESIPT turn-on. Preliminary cell stain experiment was conducted and indicated the potential biological applications.
This book is a printed edition of the Special Issue "Fluorescent Probes and Sensors" that was published in Sensors
The design and synthesis of fluorescent chemosensor probes based on photoinduced electron transfer is discussed. First, the design of a series of probes to detect the presence of divalent metal cations is discussed. Next, the results for an existing probe for divalent cations are examined. Computational chemistry is used to provide a possible answer to its selectivity. This method of examination suggests that the probes selectivity is due to the bonding involved in analyte coordination. Then, the design of a series of probes to detect the presence of mono-, di-, and, trivalent hydrocarbon anions is discussed. From this discussion, the synthesis of a single probe from that series as well as characterization and the spectrophotometric results from its titration with pyruvate, malate, and citrate is discussed, respectively. The probe was found to be more selective and sensitive toward pyruvate. Work is currently underway to design and synthesize other series of probes to detect this family of anion.
Fluorescent nucleic acid probes, which use energy transfer, include such constructs as molecular beacons, molecular break lights, Scorpion primers, TaqMan probes, and others. These probes signal detection of their targets by changing either the intensity or the color of their fluorescence. Not surpr- ingly, these luminous, multicolored probes carry more flashy names than their counterparts in the other fields of molecular biology. In recent years, fluor- cent probes and assays, which make use of energy transfer, have multiplied at a high rate and have found numerous applications. However, in spite of this explosive growth in the field, there are no manuals summarizing different p- tocols and fluorescent probe designs. In view of this, the main objective of Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is to provide such a collection. Oligonucleotides with one or several chromophore tags can form fluor- cent probes capable of energy transfer. Energy transport within the probe can occur via the resonance energy transfer mechanism, also called Förster tra- fer, or by non-Förster transfer mechanisms. Although the probes using Förster transfer were developed and used first, the later non-Förster-based probes, such as molecular beacons, now represent an attractive and widely used option. The term “fluorescent energy transfer probes” in the title of this book covers both Förster-based fluorescence resonance energy transfer (FRET) probes and probes using non-FRET mechanisms. Energy transfer probes serve as molecule-size sensors, changing their fluorescence upon detection of various DNA reactions.
Nowadays, our society is facing numerous ecological, chemical, and medical challenges that could be met with new scientific discoveries. Among these scientific studies are the qualitative and quantitative analytical determinations of chemical and biological species associated with such challenges. Design and synthesis of chemical sensors capable of operating in a complex media are of crucial importance due to their potential application in the environmental and biological processes. Fluorescence based sensors are gaining wide popularity for chemical trace detection due to their high sensitivity and low cost. Chemical sensors that operate based on specific analyte-induced changes in fluorescence appear particularly attractive because they offer the potential for detecting low analyte concentrations. The present work describes the application of different types of fluorescence-based sensors designed to distinguish various structurally similar analytes, analyte mixtures as well as specific analytes in a complex media such as blood serum and urine. Here, we describe efficient methods for sensing several analytes of interest such as ovarian cancer biomarker, cancer associated nitrosamines, drug-related amines with potential for abuse and pyrophosphate in real-time PCR monitoring. Our work is a contribution to the development of practical laboratory procedures tackling challenges of our society.
Fluorescence is the most popular technique in chemical and biological sensing and this book provides systematic knowledge of basic principles in the design of fluorescence sensing and imaging techniques together with critical analysis of recent developments. Its ultimate sensitivity, high temporal and spatial resolution and versatility enables high resolution imaging within living cells. It develops rapidly in the directions of constructing new molecular recognition units, new fluorescence reporters and in improving sensitivity of response, up to the detection of single molecules. Its application areas range from the control of industrial processes to environmental monitoring and clinical diagnostics. Being a guide for students and young researchers, it also addresses professionals involved in basic and applied research. Making a strong link between education, research and product development, this book discusses prospects for future progress.
Chemistry has become an important tool in solving problems in biology and medicine. Chemists are engaged in developing new devices at the nanoscale level to aid in clinical diagnosis, real time monitoring of analytes, and analyzing neural activity, etc. Our research is aimed at designing and synthesizing fluorescent sensors to detect various divalent metal ions under physiological conditions. We designed and synthesized a chemosensor based on the principle of photoinduced electron transfer as a signal transduction mechanism in order to detect the presence of various divalent metal cations, particularly zinc. We report the synthesis, characterization and its spectrophotometric titrations with various metal ions. This sensor signals the presence of metal ions by a fluorescence signal and offers a significant advantage as it exhibits "off-on" behavior in the presence and absence of metal ions. The primary sensor was found to be very sensitive and selective toward zinc ions. We also aimed at optimizing the performance of this chemosensor by taking advantage of electron donating/withdrawing abilities of different functional groups by using computational methods. We devised a new scheme to synthesize it, unfortunately the modified sensor was not realized for unknown reason. We are currently involved in devising an alternate strategy to synthesize and develop new sensors for neutral molecules.