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This detailed volume covers diverse aspects of P2X7 receptor analysis, ranging from its molecular structure to related pharmacological and immunological tools, via its analysis in heterologous expression systems as well as assays using primary cells and whole animal models. After three introductory chapters that focus on its structure, ligands, and physiological functions, the book details the generation of antibody and nanobody tools for P2X7 receptors, provides protocols for the analysis of expressed P2X7 receptors with a focus on their electrophysiological analysis, as well as protocols for the investigation of P2X7 down-stream signaling in immune cells by flow cytometry. Mouse models and procedures suited to investigate P2X7-mediated effects in other primary cells and in vivo are also explained. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The P2X7 Receptor: Methods and Protocols is a valuable reference not only for the growing community fascinated by this unusual ion channel but also for a broad readership interested in ion channels or purinergic receptors.
Reprint of the original, first published in 1883.
Winner of the Arthur S. Link-Warren F. Kuehl Price for Documentary Editing of the Society for Historians of American Foreign Relations The Papers of George Catlett Marshall comprise the eminent soldier and statesman's most noteworthy personal and official correspondence, speeches, congressional testimonies, and statements to the press. Volume 1 follows Marshall from childhood to his appointment as acting Chief of Staff of the US Army. It covers his education at the Virginia Military Institute and early service in France, China and the Philippines, as well as his experiences as aide-de-camp to General John J. Pershing and as assistant commandant of the Infantry School as Fort Benning, Georgia, where he mentored future leaders such as Omar Bradley, Matthew Ridgway, Joseph Stilwell and, Walter Bedell Smith.
In December 2003 the painter Jack Vettriano, a coalminer’s son, met his parents off the train from Scotland on his way to collect an OBE. Over the last few years Vettriano has had a meteoric rise to fame – emerging from the unlikely background of the Scottish coalfields, unknown and untutored, he has become Scotland’s most successful and controversial contemporary artist. Appearing on posters and cards, mugs and umbrellas, prints of his work outsell Van Gogh, Dali and Monet and his paintings have been acquired by celebrities around the world. 'The Singing Butler', Britain's most reproduced painting, fetched a record £744,800 at auction in April 2004. Vettriano’s images have an often mysterious narrative and are a gateway to an alluring yet sinister world. Daylight scenes of heady optimism, painted against backdrops of beaches and racetracks, are counterbalanced by more disquieting canvases of complex night-time liaisons in bars and clubs, bedrooms and ballrooms. Both sexes are clearly styled – the men hard-edged and mysterious, the women seductive and enigmatic. Yet beneath the confident posturing, Vettriano recognizes our inherent human frailty, that there is no victor in the struggle between duplicity and desire. Men and women are ultimately trapped by the machinations of intense love and passion with little control over their destiny. 'Jack Vettriano' presents about thirty new images, as well as some recently surfaced works, plus the best of the paintings previously published in 'Lovers and Other Strangers' and 'Fallen Angels', also by Pavilion. In March 2004 Melvin Bragg’s The South Bank Show broadcast a programme dedicated to Jack entitled Jack Vettriano: The People’s Painter. Reissued in smaller user-friendly format.