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This paper provides guidelines for new high-throughput screening methods – both phenotypic and genotypic – to enable the detection of rare mutant traits, and reviews techniques for increasing the efficiency of crop mutation breeding.
This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.
Abstract: This book presents contemporary information on mutagenesis in plants and its applications in plant breeding and research. The topics are classified into sections focusing on the concepts, historical development and genetic basis of plant mutation breeding (chapters 1-6); mutagens and induced mutagenesis (chapters 7-13); mutation induction and mutant development (chapters 14-23); mutation breeding (chapters 24-34); or mutations in functional genomics (chapters 35-41). This book is an essential reference for those who are conducting research on mutagenesis as an approach to improving or modifying a trait, or achieving basic understanding of a pathway for a trait --.
Genetic variability is an important parameter for plant breeders in any con ventional crop improvement programme. Very often the desired variation is un available in the right combination, or simply does not exist at all. However, plant breeders have successfully recombined the desired genes from cultivated crop gerrnplasm and related wild species by sexual hybridization, and have been able to develop new cultivars with desirable agronomie traits, such as high yield, disease, pest, and drought resistance. So far, conventional breeding methods have managed to feed the world's ever-growing population. Continued population growth, no further scope of expanding arable land, soil degradation, environ mental pollution and global warrning are causes of concern to plant biologists and planners. Plant breeders are under continuous pressure to improve and develop new cultivars for sustainable food production. However, it takes several years to develop a new cultivar. Therefore, they have to look for new technologies, which could be combined with conventional methods to create more genetic variability, and reduce the time in developing new cultivars, with early-maturity, and improved yield. The first report on induced mutation of a gene by HJ. Muller in 1927 was a major mi1estone in enhancing variation, and also indicated the potential applica tions of mutagenesis in plant improvement. Radiation sources, such as X-rays, gamma rays and fast neutrons, and chemical mutagens (e. g. , ethyl methane sulphonate) have been widely used to induce mutations.
An essential and comprehensive summary for all plant breeders.
The main objective of this book is to bring all the research activities of mutation breeding in one umbrella.
Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.
The Indian Society of Genetics and Plant Breeding was established in 1941 in recognition of the growing contribution of improved crop varieties to the country's agriculture. Scientific plant breeding had started inIndia soon after the rediscovery of Mendel's laws of heredity. The Indian Agricultural Research Institute set up in 1905 and a number of Agricultural Colleges in different parts of the country carried out some of the earliest work mostly inthe form of pure-line selections. In subsequent years, hybridization programmes in crops like wheat, rice, oilseeds, grain legumes, sugarcane and cotton yielded a large number of improved cultivars with significantly higher yields. A turning point came in the 1960s with the development of hybrids in several crops including inter-specific hybrids in cotton. And when new germplasm with dwarfing genes became available in wheat and rice from CIMMYT and IRRI, respectively,Indian plant breeders quickly incorporated these genes into the genetic background of the country's widely grown varieties with excellent grain quality and other desirable traits. This was to mark the beginning of modem agriculture in India as more and more varieties were developed, characterized by a high harvest index and response to modem farm inputs like the inorganic fertilizers . India's green revolution which has led to major surpluses offood grains and othercommodities like sugar and cotton has been made possible by the work of one of the largest groups of plant breeders working in a coordinated network.
Induced mutagenesis is a common and promising method for the screening of new crops with improved production methods, and has made a tremendous contribution to crop improvement. Now, as the techniques of molecular biology become more widely adopted by plant breeders, this comprehensive summary sets mutation breeding within a contemporary context and relates it to other breeding techniques. This book opens a new chapter of inducing mutations at the gene level, and details techniques that can be used to harvest and exploit such mutation to improve the productivity of crops, particularly cereals, grains and vegetables. The chapters within this volume are supported by diagrams, tables and graphs to make the content more comprehensible. The book will be extremely useful for advanced undergraduates, graduates, postgraduate students, and research scientists of botany, agriculture, horticulture, genetics, biotechnology, biochemistry and agronomy.