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Fluorescence imaging and spectroscopy is an important tool in many areas of research. Biology has particularly benefitted from fluorescence techniques, since a single molecule's position, local environment, and even activity can be studied in real time by tagging it with a fluorescent label. It is, therefore, important to be able to understand and manipulate fluorescence. One way to control fluorescence is to shape the local electromagnetic fields that excite the fluorescent molecule. This thesis studies the interaction between fluorescent molecules and two nanophotonic structures that highly modify local electromagnetic fields: the bowtie nanoantenna and the photonic crystal cavity. The study of plasmons, or coherent excitations of free electrons in a metal, has led to the fabrication of antennas at optical frequencies. In particular, gold bowtie nanoantennas have been shown to concentrate light from the diffraction limit at 800 nm (~300 nm) down to ~20 nm, while also enhancing the local electric field intensity by a factor of 1,000. This huge change in the local field greatly alters the absorption and fluorescence emission of nearby molecules. This thesis will show that the fluorescence from an initially-poor single-molecule emitter can be enhanced by a factor of 1,300, allowing for the measurement of one highly enhanced molecule over a background of 1,000 unenhanced molecules. By extending this experiment to molecules in solution, dynamics of single molecules in concentrated solutions can also be measured. While bowtie nanoantennas act to concentrate light, light does not remain in the structure for long. The photonic crystal cavity can be used to trap and store light, which has interesting implications for molecular emitters located nearby. This thesis will show that molecules can be lithographically positioned onto a photonic crystal cavity and that the molecule's fluorescence emission is coupled to the cavity modes.
Discover how metal-enhanced fluorescence is changing traditional concepts of fluorescence This book collects and analyzes all the current trends, opinions, and emerging hot topics in the field of metal-enhanced fluorescence (MEF). Readers learn how this emerging technology enhances the utility of current fluorescence-based approaches. For example, MEF can be used to better detect and track specific molecules that may be present in very low quantities in either clinical samples or biological systems. Author Chris Geddes, a noted pioneer in the field, not only explains the fundamentals of metal-enhanced fluorescence, but also the significance of all the most recent findings and models in the field. Metal-enhanced fluorescence refers to the use of metal colloids and nanoscale metallic particles in fluorescence systems. It offers researchers the opportunity to modify the basic properties of fluorophores in both near- and far-field fluorescence formats. Benefits of metal-enhanced fluorescence compared to traditional fluorescence include: Increased efficiency of fluorescence emission Increased detection sensitivity Protect against fluorophore photobleaching Applicability to almost any molecule, including both intrinsic and extrinsic chromophores Following a discussion of the principles and fundamentals, the author examines the process and applications of metal-enhanced fluorescence. Throughout the book, references lead to the primary literature, facilitating in-depth investigations into particular topics. Guiding readers from the basics to state-of-the-technology applications, this book is recommended for all chemists, physicists, and biomedical engineers working in the field of fluorescence.
Written by the leading experts in the field, this book describes the development and current state of the art in single molecule spectroscopy. The application of this technique, which started 1989, in physics, chemistry and biosciences is displayed.
Explains the principles and current thinking behind plasmon enhanced Fluorescence Describes the current developments in Surface Plasmon Enhanced, Coupled and Controlled Fluorescence Details methods used to understand solar energy conversion, detect and quantify DNA more quickly and accurately, and enhance the timeliness and accuracy of digital immunoassays Contains contributions by the world’s leading scientists in the area of fluorescence and plasmonics Describes detailed experimental procedures for developing both surfaces and nanoparticles for applications in metal-enhanced fluorescence
This book highlights the various topics in which luminescence and electrochemistry are intimately coupled. The topic of this book is clearly at the frontier between several scientific domains involving physics, chemistry and biology. Applications in these various fields naturally also need to be mentioned, especially concerning displays and advanced investigation techniques in analytical chemistry or for biomedical issues.
This book discusses a new class of photonic devices, known as surface plasmon nanophotonic structures. The book highlights several exciting new discoveries, while providing a clear discussion of the underlying physics, the nanofabrication issues, and the materials considerations involved in designing plasmonic devices with new functionality. Chapters written by the leaders in the field of plasmonics provide a solid background to each topic.
This volume brings together several recent research articles in the field of nanophotonics. The editors have arranged the chapters in three main parts: quantum devices, photonic devices, and semiconductor devices. The chapters cover a wide variety of scopes in those areas including principles of plasmonic, SPR, LSPR and their applications, graphene-based nanophotonic devices, generation of entangled photon and quantum dots, perovskite solar cells, photo-detachment and photoionization of two-electrons systems, diffusion and intermixing of atoms in semiconductor crystals, lattice and molecular elastic and inelastic scattering including surface-enhanced Raman Scattering and their applications. It is our sincerest hope that science and engineering students and researchers could benefit from the new ideas and recent advances in the field that are covered in this book.
This book explores the role of surface effects in optical phenomena in nanoscience, from two different perspectives. When systems are reduced in volume, the ratio of surface versus volume increases. At the level of single nanostructures this translates into an enhanced role of interfacial chemistry and thermodynamics. At the level of systems of nanostructures, it translates into larger density on interfaces, which in turn leads to such intriguing collective effects as plasmonics or multiple reflection and refraction phenomena. The book highlights both perspectives presenting sample applications. Without claiming to be exhaustive, the book aims to stimulate readers in this potentially rewarding field.
Time-correlated Single Photon Counting has been written in the hope that by relating the authors' experiences with a variety of different single photon counting systems, they may provide a useful service to users and potential users of this formidably sensitive technique. Of all the techniques available to obtain information on the rates of depopulation of excited electronic singlet states of molecular species, monitoring of fluorescence provides, in principle, the simplest and most direct measure of concentration. This volume comprises eight chapters, with the first focusing on the time dependence and applications of fluorescence. Succeeding chapters go on to discuss basic principles of the single photon counting lifetime measurement; light sources; photomultipliers; electronics; data analysis; nanosecond time-resolved emission spectroscopy; time dependence of fluorescence anisotropy. This book will be of interest to practitioners in the field of chemistry.
This 21st Century Nanoscience Handbook will be the most comprehensive, up-to-date large reference work for the field of nanoscience. Handbook of Nanophysics, by the same editor, published in the fall of 2010, was embraced as the first comprehensive reference to consider both fundamental and applied aspects of nanophysics. This follow-up project has been conceived as a necessary expansion and full update that considers the significant advances made in the field since 2010. It goes well beyond the physics as warranted by recent developments in the field. Key Features: Provides the most comprehensive, up-to-date large reference work for the field. Chapters written by international experts in the field. Emphasises presentation and real results and applications. This handbook distinguishes itself from other works by its breadth of coverage, readability and timely topics. The intended readership is very broad, from students and instructors to engineers, physicists, chemists, biologists, biomedical researchers, industry professionals, governmental scientists, and others whose work is impacted by nanotechnology. It will be an indispensable resource in academic, government, and industry libraries worldwide. The fields impacted by nanoscience extend from materials science and engineering to biotechnology, biomedical engineering, medicine, electrical engineering, pharmaceutical science, computer technology, aerospace engineering, mechanical engineering, food science, and beyond.