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This work is a comprehensive, international review of new immunoassay technologies. Examines special aspects of the subject, such as the large-scale production of novel monoclonal antibodies, the influence of reference preparations and matrix effects, and aspects of solid-phase chemistry. Covers the development of practical tests for use in extra-laboratory locations, and the recent availability of ultrasensitive, semi-automatic, laboratory procedures. Coverage of the problems of terminology and classification, and a glossary of 144 specialized terms are included.
Handbook of Immunoassay Technologies: Approaches, Performances, and Applications unravels the role of immunoassays in the biochemical sciences. During the last four decades, a wide range of immunoassays has been developed, ranging from the conventional enzyme-linked immunosorbent assays, to the smartphone-based point-of-care formats. The advances in rapid biochemical procedures, novel biosensing schemes, fully integrated lab-on-a-chip platforms, prolonged biomolecular storage strategies, device miniaturization and interfacing, and emerging smart system technologies equipped with personalized mobile healthcare tools are paving the way to next-generation immunoassays, and are all discussed in this comprehensive text. Immunoassays play a prominent role in clinical diagnostics as they are the eyes of healthcare professionals, helping them make informed clinical decisions via confirmed disease diagnosis, and thus enabling favorable health outcomes. The faster and reliable diagnosis of infections will further control their spread to uninfected persons. Similarly, immunoassays play a prominent role in veterinary diagnostics, food analysis, environmental monitoring, defense and security, and other bioanalytical settings. Therefore, they enable the detection of a plethora of analytes, which includes disease biomarkers, pathogens, drug impurities, environmental contaminants, allergens, food adulterants, drugs of abuse and various biomolecules. - Provides a valuable increase of understanding of cellular and biomedical functions - Gives the most updated resource in the field of immunoassays, providing the comprehensive details of various types of immunoassays that need to be performed in healthcare, and in industrial, environmental and other biochemical settings - Discusses all multifarious aspects of immunoassays - Describes the immunoassay formats, along with their principle of operation, characteristics, pros and cons, and potential biochemical and bioanalytical applications - Provides extensive knowledge and guided insights as detailed by experienced, renowned experts and key opinion makers in the field of immunoassays
Each chapter of this book aims to explore the basic physical and chemical principles involved in the immunoassay techniques discussed. The book also looks at the optimization and limitations of methodology and concludes with a brief overview of the application of the performance of the technology.
"Blood transfusion is a life-saving intervention that has an essential role in patient management within health care systems. All Member States of the World Health Organization (WHO) endorsed World Health Assembly resolutions WHA28.72 (1) in 1975 and WHA58.13 (2) in 2005. These commit them to the provision of adequate supplies of safe blood and blood products that are accessible to all patients who require transfusion either to save their lives or promote their continuing or improving health." --Preface.
Companion and Complementary Diagnostics: From Biomarker Discovery to Clinical Implementation provides readers with in-depth insights into the individual steps in the development of companion diagnostic assays, from the early biomarker discovery phase straight through to final regulatory approval. Further, the clinical implementation of companion diagnostic testing in the clinic is also discussed. As the development of predictive or selective biomarker assays linked to specific drugs is substantially increasing, this book offers comprehensive information on this quickly-evolving area of biomedicine. It is an essential resource for those in academic institutions, hospitals and pharma, and biotech and diagnostic commercial companies. - Covers all aspects, from biomarker discovery, to development and regulatory approval - Explains the "how to" aspects of companion diagnostics - Incorporates information on the entire process, allowing for easier and deeper understanding of the topic
Practical Immunology is a basic text aimed at immunology students and researchers at all levels who need a comprehensive overview of the methodology of immunology. The rapid and startling innovations in immunology over the past two decades have their root in sound experimental practice and it has always been the aim of this book to educate researchers in the design and performance of complex techniques. It will appeal to students of immunlogy, graduate students embarking on bench science, or specialised immunologists who need to use an immunological technique outside their sphere of expertise. The definitive lab "bench book". A one stop resource. Techniques explained from first principles. Basic forms of apparatus described in detail. Totally revised with new user friendly layout to aid use in the lab. Includes useful hints and tips.
Immunochemistry of Solid-Phase Immunoassay fills a niché in the field of immunoassay and immunology. Although solid-phase immunoassay constitutes a major technology in biology and medicine, there is no comprehensive source devoted to the immunochemical principles involved. As a result, this book will benefit students, technicians, and researchers who use this technology, as well as immunodiagnostic and biotech companies who develop the technology. The book is not a methods manual; instead, it incorporates the concepts, data, and opinions of more than 25 investigators working in this field. Topics discussed include: the chemistry of solid-phases, the behavior or antibodies and antigens on solid phases, membrane solid-phases, reaction kinetics, antigen quantitation, enzyme systems, photophysics, immunochemical considerations in data analyses, multianalyte assays and occupancy concepts, antibody quantitation, streptavidin, a review of data analysis software, and solid-phase peptide immunoassay.
Salivary Diagnostics surveys one of the most exciting areas of research in oral biology. Regarded as the mirror of the body, saliva has immense potential to yield real clinical improvements in our ability to diagnose, and hence treat, oral and systemic conditions. The composition of saliva and other oral fluids reflects the tissue fluid levels of therapeutic, hormonal, and immunological molecules, as well as the presence of markers for systemic and oral disease.
This book presents the results of a comprehensive study of literature on patents, patent applications and other literature, such as journal articles. The survey covers literature published from the United States, Japan and Western Europe between January 1980 and April 1991.
This book discusses in detail various serological tests that are used to identify medical conditions and diseases, from a general overview of each test to the equipment and steps needed to carry them out. The book is aimed specifically at immunology students and professionals who may occasionally need to use these tests, and thus lack training and experience in performing them. The book provides a brief overview of the immune system, including antibodies, antigens, and their interactions. The bulk of the book is comprised of 16 chapters that each explain different serological tests. These chapters start with a general introduction of the test or disease being detected, followed by the test principle, reagents required for the test, procedures and steps to perform the test, and, finally, result interpretation. Both test principles and result interpretation segments include illustrations to aid comprehension. In addition, the book also enables the reader to distinguish between positive and negative results in serological testing.