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A versatile collection of readily reproducible cell-cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo. The protocols cover a diverse set of cell-cell interaction models in both normal and pathological states, are readily adaptable to nearly any cell type and organ system, and include primary data and outcome analysis. In addition, the protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
H. F. LINSKENS and J. HESLOP-HARRISON The chapters of this volume deal with intercellular interaction phenomena in plants. Collectively they provide a broad conspectus of a highly active, if greatly fragmented, research field. Certain limitations have been imposed on the subject matter, the most impor tant being the exclusion of long-range interactions within the plant body. It is true that pervasive hormonal control systems cannot readily be demarcated from controls mediated by pheromones or information-carrying molecules with more limited spheres of action, but consideration is given in this volume to the main classes of plant hormones and their functions only incidentally, since these are treated adequately in other volumes of this Encyclopedia series (V - ume 9-11) and in numerous other texts and reviews. Similarly, certain other effects, such as those associated with nutrients and ions, are not considered in any detail. Furthermore, we have excluded intracellular interactions, and also consideration of transport phenomena, which are treated in detail in Vol ume 3 of this Series. Other aspects of inter-cellular interaction, such as cell surface phenomena and implications of lectin-carbohydrate interactions, and plant-virus inter-relationships, are treated in other sections of this Encyclopedia (Volumes 13B and 14B, respectively). In the volume on physiological plant pathology (Volume 4 of this series) special attention has been given to host pathogen interaction. These aspects of our subject will therefore be excluded in the present treatise.
Mechanobiology of Cell-Matrix Interactions focuses on characterization and modeling of interactions between cells and their local extracellular environment, exploring how these interactions may mediate cell behavior. Studies of cell-matrix interactions rely on integrating engineering, (molecular and cellular) biology, and imaging disciplines. Recent advances in the field have begun to unravel our understanding of how cells gather information from their surrounding environment, and how they interrogate such information during the cell fate decision making process. Topics include adhesive and integrin-ligand interactions; extracellular influences on cell biology and behavior; cooperative mechanisms of cell-cell and cell-matrix interactions; the mechanobiology of pathological processes; (multi-scale) modeling approaches to describe the complexity or cell-matrix interactions; and quantitative methods required for such experimental and modeling studies.
Aimed at an international audience of researchers, this book contains a mixture of general discussion and experimental protocol. The techniques discussed cover a broad range of molecular and organismal biology, and allow the manipulation of developing organisms.
The NATO Advanced Study Institute on "Cellular and Molecular Control of Direct Cell Interactions in Developing System" has been attended by 15 invited main lecturers and 60 participants. According to its purpose senior scientists, postdoctoral trainees and graduate students working in areas like biology, biochemistry, electrophysiology, medicine etc . . . could discuss their common interest in the various structural, ultrastructural, molecular and functional aspects of cell interactions in developing in vivo and in vitro systems. Whereas the topics of the first week have been mostly concerned with the general aspects of cell interactions in embryogenesis (section I and II of this book), the second week has been mainly devoted to the structures and functions of the direct cell contact sites at the membrane level as gap junctions, including electrophysiological aspects, dye coupling and selective cell-cooperation in some model systems as the neuro-muscular junctions (section III-V of this book). A multidisciplinary and stepwise approach, from initial cell contacts in early embryogenesis up to well defined selective cell cooperation, appeared to be an efficient means to provide answers to the question of how cells control, in a dynamic system as given in a differentiating embryo, their multiple temporary and permanent interactions so necessary for ordered cell positioning, cell linking and well established cell-to-cell communication.
Cancer was thought to originate from alterations in intercellular signaling that resulted in the transformation of cells, their uncontrolled proliferation and metastasis. There is now an increasing body of evidence demonstrating that the surrounding matrix and cell-matrix interactions are also major players in this process. Cells adhere and receive signals from various extracellular matrices via transmembrane receptors, the best known of which are the heterodimeric glycoproteins, integrins.
H. F. LINSKENS and J. HESLOP-HARRISON The chapters of this volume deal with intercellular interaction phenomena in plants. Collectively they provide a broad conspectus of a highly active, if greatly fragmented, research field. Certain limitations have been imposed on the subject matter, the most impor tant being the exclusion of long-range interactions within the plant body. It is true that pervasive hormonal control systems cannot readily be demarcated from controls mediated by pheromones or information-carrying molecules with more limited spheres of action, but consideration is given in this volume to the main classes of plant hormones and their functions only incidentally, since these are treated adequately in other volumes of this Encyclopedia series (V - ume 9-11) and in numerous other texts and reviews. Similarly, certain other effects, such as those associated with nutrients and ions, are not considered in any detail. Furthermore, we have excluded intracellular interactions, and also consideration of transport phenomena, which are treated in detail in Vol ume 3 of this Series. Other aspects of inter-cellular interaction, such as cell surface phenomena and implications of lectin-carbohydrate interactions, and plant-virus inter-relationships, are treated in other sections of this Encyclopedia (Volumes 13B and 14B, respectively). In the volume on physiological plant pathology (Volume 4 of this series) special attention has been given to host pathogen interaction. These aspects of our subject will therefore be excluded in the present treatise.
Many advances have been made in the last decade in the understanding of the computational principles underlying olfactory system functioning. Neuromorphic Olfaction is a collaboration among European researchers who, through NEUROCHEM (Fp7-Grant Agreement Number 216916)-a challenging and innovative European-funded project-introduce novel computing p
A guide to methods of identification and characterization of the molecules that mediate cellular interactions in a variety of systems. Methods are included for preparing probes, analyzing adhesive and junctional interactions, and determining functionally important structural features of molecules.