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Cell Growth, Nutrition, Cytodifferentiation, and Cryopreservation
The Photosynthetic Apparatus: Molecular Biology and Operation: Cell Culture and Somatic Cell Genetics of Plants, Volume 7B is a collection of papers that discuss plastids – organelles found in plants that set them apart from other organisms. The book is divided into two parts. Coverage of Part I includes concepts such as photosynthesis and the photosynthetic apparatus - light energy and photosynthetic electronic transport, photosynthetic phosphorylation, and fractionation of the photosynthetic apparatus; photosystem II – its protein components, genetic aspects, and structure and function; the cytochrome b6/f complex; and the structure and function of coupling factor components. Coverage of Part II includes the biochemistry and molecular biology of chlorophyll; genes and enzymes for carotenoid biosynthesis; photoregulated development of chloroplasts; and the differentiation of amyloplasts and chromoplasts. The text is recommended for botanists, molecular biologists, and biochemists who are interested in the study of plant cells and photosynthesis.
The Molecular Biology of Plastids: Cell Culture and Somatic Cell Genetics of Plants, Volume 7A deals with various aspects of plastid nucleic acid and protein metabolism. This book is organized into 10 chapters. Chapter 1 provides the introduction to the molecular biology of plastids, followed by a discussion of the maps of restriction endonuclease sites on chloroplast chromosomes in Chapter 2. Chapter 3 focuses on chloroplast gene transmission, while Chapters 4 to 7 describe the apparatus for nucleic acid and protein metabolism and how some transcripts of chloroplast genes are processed. The ribosomal proteins, ribosomes, and translation in plastids are covered in Chapter 8. The last two chapters consider the organization, operation, and transport of polypeptides through the outer plastid membranes. This volume is a good reference for plant molecular biologist, genetic engineers, and researchers conducting work on the molecular biology of chloroplasts.
Scale-Up and Automation in Plant Propagation reviews methods of automation and scale-up of plant propagation in vitro. It looks at the large scale clonal propagation of plants, or micropropagation, as the first major practical application of plant biotechnology. It also discusses the advantages and limitations of micropropagation and evaluates current methods of commercial micropropagation. Organized into 13 chapters, this volume begins with an overview of the benefits of scaling up and automating plant propagation before proceeding with a discussion of synthetic seeds and their use for plant propagation, along with problems and economic considerations associated with synthetic seed technology. It then considers the implementation of somatic embryogenesis technology for clonal forestry, the development and commercialization of bioreactor technology for automated propagation of potato microtubers and lily microbulbs, and approaches to automated propagation of fruit trees. Other chapters focus on issues of cost reduction and development of ""new"" products, scale-up and operation of prototype bioreactors for plant propagation, and application of machine vision technology to scale-up and automated evaluation of somatic embryogenesis in sweet potato. The book also describes methods of measurement and control of the environment in culture, environmental factors affecting photosynthesis, and use of robotics and field transplanters in the automation of plant propagation. Scientists and plant breeders will find this book extremely useful.
Almost a decade has passed since the last textbook on the science of cryobiology, Life in the Frozen State, was published. Recently, there have been some serious tectonic shifts in cryobiology which were perhaps not seen on the surface but will have a profound effect on both the future of cryobiology and the development of new cryopreservation methods. We feel that it is time to revise the previous paradigms and dogmas, discuss the conceptually new cryobiological ideas, and introduce the recently emerged practical protocols for cryopreservation. The present books, "Current Frontiers in Cryobiology" and "Current Frontiers in Cryopreservation" will serve the purpose. This is a global effort by scientists from 27 countries from all continents and we hope it will be interesting to a wide audience.
This manual comprises a broad range of techniques of value to research workers in the fields of cell and molecular biology, physiology, plant breeding and propagation, and genetic engineering.
The aim of this volume is to merge classical concepts of plant cell biology with the recent findings of molecular studies and real-world applications in a form attractive not only to specialists in the realm of fundamental research, but also to breeders and plant producers. Four sections deal with the control of development, the control of stress tolerance, the control of metabolic activity, and novel additions to the toolbox of modern plant cell biology in an exemplary and comprehensive manner and are targeted at a broad professional community. It serves as a clear example that a sustainable solution to the problems of food security must be firmly rooted in modern, continuously self re-evaluating cell-biological research. No green biotech without green cell biology. As advances in modern medicine is based on extensive knowledge of animal molecular cell biology, we need to understand the hidden laws of plant cells in order to handle crops, vegetables and forest trees. We need to exploit, not only empirically, their astounding developmental, physiological and metabolic plasticity, which allows plants to cope with environmental challenges and to restore flexible, but robust self-organisation.
The papers in this book, illustating the present status of knowledge related to the vacuolar compartment of fungi and plants, were presented at an Advanced Research Workshop entitled "Plant Vacuoles. Their Importance in Plant Cell Compartmentation and their Applications in Biotechnology" held in Sophia-Antipolis, France, on July 6-11, 1986. The organizers were fortunate in being able to assemble representative leaders of all the above fields of research concerning this compartment. These scientists from all over the world were invited to present their latest results and to exchange views and plans for continued research in this highly exciting field, which is very important for the improving the industrial aspect of plant biotechnology related to the production of molecules with a high added value. To ensure maximal flexibility and opportunity for dicussion, the chairmen of the various sessions were asked to introduce their respective topics, after which they were given the freedom of organizing their sessions in a more or less improvised fashion, with brief presentations of relevant new information by participants following a schedule fixed relatively shortly before the workshop. This procedure proved to be highly successful, giving rise to most productive and stimulating discussions among the representatives of the various fields.
A number of interdisciplinary fields related to Plant Cell Biotechnology are discussed. The two main directions are: Plant cell culture in agricultural applications for the improvement of crops and industrial applications in the production of secondary metabolites. A number of areas such as physiological and biochemical aspects of autotrophic cells, gene characterization in higher plants, transformation of plant cells, genetic stability in plant cell cultures, somatic hybridization and somatic embryogenesis are treated. Recent knowledge on somaclonal and gametoclonal variation as well as on the obtainment of protoplasts and their use for the isolation and culture of heterocaryons as tools for plant breeding are considered. Furthermore, the knowledge on biomass production in fermentor conditions and the role of immobilization for increased production and scale-up of plant cells are discussed.