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Biochemical Pathways and Environmental Responses in Plants: Part C, Volume 683 in the Methods in Enzymology series, highlights advances in the field. with this new volume presenting chapters on topics including Preparation of hydroxycinnamoyl-CoA thioesters using recombinant 4-coumarate:coenzyme A ligase (4CL) for characterization of BAHD acyltransferases, Near-real time determination of BAHD acyl-coA transferase reaction rates, Bioinformatic tools for protein structure prediction and for molecular docking applied to enzyme active site analysis, Computational and biochemical methods to measure the activity of carboxysomes and protein organelles in vivo, Dirigent Proteins Family Function and Structure, Three-in-One Method for High-throughput plant multi-omics, amongst other timely topics. Additional sections cover Analysis of isoprenyl-phosphates by liquid chromatography-mass spectrometry, Lignin/Lignan Biosynthesis Structure/Function, Comparison of TLC, HPLC, and direct infusion mass spectrometry methods for identification and quantification of diacylglycerol molecular species, Plant sphingolipid analysis, RNA-seq analysis of alternative pre-mRNA splicing mediated by photoreceptors in Physcomitrium patens, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in Methods in Enzymology serials - Updated release includes the latest information on Biochemical Pathways and Environmental Responses in Plants
Biochemical Pathways and Environmental Responses in Plants, Part B, Volume 682 in the Methods in Enzymology series, highlights advances in the field with this new volume presenting chapters on MIE 681/682: Biochemical pathways and environmental responses in plants, Structure, function, and engineering of plant polyketide synthases, A sensitive LC-MS/MS assay for enzymatic characterization of methylthioalkylmalate synthase involved in glucosinolate side-chain elongation, Assaying formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based assay, An Approach to Nearest Neighbor Analysis of Pigmented Protein Complexes by Using Chemical Crosslinking in Combination with Mass Spectrometry, Biochemical characterization of plant aromatic aminotransferases, and much more. Other chapters focus on Functional Analysis of Phosphoethanolamine N-methyltransferase (PMT) in Plants and Parasites, A structure-guided computational screening approach for predicting plant enzyme-metabolite interactions, Plant metacaspase: an example of microcrystal structure determination and analysis, Biocatalytic system for comparative assessment of functional association of cytochrome P450 monooxygenases with their redox partners, Dirigent Protein Family Function and Structure, and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in Methods in Enzymology series - Includes the latest information on Biochemical pathways and environmental responses in plants
Biochemical Pathways and Environmental Responses in Plants, Part A, Volume 676 in the Methods in Enzymology series highlights new advances in the field with this new volume presenting interesting chapters on topics such as Structure, function, and engineering of plant polyketide synthases, A sensitive LC-MS/MS assay for enzymatic characterization of methylthioalkylmalate synthase involved in glucosinolate side-chain elongation, Assaying formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based assay, An Approach to Nearest Neighbor Analysis of Pigmented Protein Complexes by Using Chemical Crosslinking in Combination with Mass Spectrometry, and much more. Other chapters cover Biochemical characterization of plant aromatic aminotransferases, Functional Analysis of Phosphoethanolamine N-methyltransferase (PMT) in Plants and Parasites, A structure-guided computational screening approach for predicting plant enzyme-metabolite interactions, Plant metacaspase: an example of microcrystal structure determination and analysis, Biocatalytic system for comparative assessment of functional association of cytochrome P450 monooxygenases with their redox partners, Dirigent Protein Family Function and Structure, and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in Methods in Enzymology series - Includes the latest information on Biochemical pathways and environmental responses in plants
Respiration in plants, as in all living organisms, is essential to provide metabolic energy and carbon skeletons for growth and maintenance. As such, respiration is an essential component of a plant’s carbon budget. Depending on species and environmental conditions, it consumes 25-75% of all the carbohydrates produced in photosynthesis – even more at extremely slow growth rates. Respiration in plants can also proceed in a manner that produces neither metabolic energy nor carbon skeletons, but heat. This type of respiration involves the cyanide-resistant, alternative oxidase; it is unique to plants, and resides in the mitochondria. The activity of this alternative pathway can be measured based on a difference in fractionation of oxygen isotopes between the cytochrome and the alternative oxidase. Heat production is important in some flowers to attract pollinators; however, the alternative oxidase also plays a major role in leaves and roots of most plants. A common thread throughout this volume is to link respiration, including alternative oxidase activity, to plant functioning in different environments.
Nutraceuticals and Natural Product Pharmaceuticals analyzes the nutraceutical and pharmaceutical research published over the last decade, paying particular attention to applications and recovery effects. The book emphasizes the great need for both nutritionists and pharmacologists to understand how these drugs can benefit human health. Topics explore innovative sources, bioavailability, pharmacokinetics, translating novel pathways and mechanisms of action into their clinical use, personalized nutrition and natural product medicine, the convergence between nutraceuticals and western medicine, interactions between drugs, nutrients, the microbiome and lifestyles, industrial applications and commercialization, metabolomics, nano-delivery systems and function, and more. Nutritionists and pharmacists working with natural products, food scientists, nutrition researchers and those interested in the development of innovative products, nutraceuticals, pharmaceuticals and functional foods are sure to benefit from this thorough resource. - Connects research from the nutraceutical and pharmaceutical industries - Promotes further communication and cooperation between pharmacologists and nutritionists by analyzing nutraceutical and pharmaceutical research in particular applications and recovery efforts - Explores the health effects of target compounds and the development of applications in both sectors
The Fourth Edition of the Handbook of Photosynthesis offers a unique and comprehensive collection of topics in the field of photosynthesis, serving as an invaluable resource in this field. With contributions from 95 scientists and experts from over 20 countries, this volume has been divided into 13 parts, each serving independently to facilitate the understanding of the material. FEATURES Presents comprehensive information on photosynthesis under normal and environmental stress conditions Covers artificial photosynthesis and its future-related issues Contains 25 new chapters and 18 extensively revised and expanded chapters Includes three new sections: Influence of Nanoparticles on Photosynthesis; Protection of Photosynthesis System and Stress Alleviation Strategies by Photosynthates Manipulations and Photosynthesis Efficiency in Plants under Multiple Abiotic and Biotic Stressors; and Artificial Photosynthesis and Its Future Contains numerous tables, figures, illustrations, and case studies to facilitate the comprehension of the material as well as thousands of index words A primary resource in its field, Handbook of Photosynthesis, Fourth Edition, provides a comprehensive resource for researchers, academics, and for university courses, with the information as a valuable source to plan, implement, and evaluate strategies for dealing with photosynthesis issues.
This Special Issue of Water, Air and Soil Pollution offers contributions from the th 18 IUFRO workshop on Air Pollution Stress, Forest Responses to the Pollution st Climate of the 21 Century held in Edinburgh, Scotland, from September 21 to 23,1998. The meeting was held under the auspices of IUFRO, Research Group 7.04.00 chaired by Dr Kevin Percy of Canada. A new session structure was adopted to stimulate activity within the six working parties and a brief resume of these is presented at the front of this volume. The two, one-day plenary sessions were devoted to the two important air pollution issues, nitrogen deposition and ozone. Invited papers were augmented by a large and excellent contribution of poster papers. The final day comprised parallel Working Party Sessions with pre arranged speakers to stimulate discussions. One hundred and thirty one scientists attended, representing 20 countries and 7 IUFRO regions: Northern Europe, Central Europe, Eastern Europe, Mediterranean, North America, Asia and the Western Pacific. Lucy Sheppard David Fowler Water, Air, and Soil Pollution 116: 1, 1999.
Fruits play a substantial role in the human diet as a source of vitamins, minerals, dietary fiber and a wide range of molecules relevant to health promotion and disease prevention. The characterization of genes involved in the accumulation of these molecules during fruit development and ripening, and in the overall plant’s response to the environment, constitutes a fundamental step for improving yield- and quality-related traits, and for predicting this crop’s behavior in the field. This is certainly the case for grapevine (Vitis vinifera L.), one of the most largely cultivated fruit crops in the world. The cultivation of this species is facing challenging scenarios driven by climate change – including increases in atmospheric carbon dioxide (CO2), solar radiation, and earth surface temperature, and decreases of water and nutrient availability. All these events will potentially affect the grapevine phenology, physiology, and metabolism in many growing regions and ultimately affect the quality of their fruits and of the most important derived product, the wine. The sequencing of the grapevine genome has given rise to a new era, characterized by the generation of large-scale data that requires complex computational analyses. Numerous transcriptomic and metabolomic studies have been performed in the past fifteen years, providing insights into the gene circuits that control the accumulation of all sorts of metabolites in grapevines. From now on, the integration of two or more ‘omics’ will allow depicting gene-transcript-metabolite networks from a more holistic (i.e. systems) perspective. This eBook attempts to support this new direction, by gathering innovative studies that assess the impact of genotypes, the environment, and agronomical practices on fruits at the ‘ome’-scale. The works hereby collected are part of a Research Topic covering the use of ‘omics’-driven strategies to understand how environmental factors and agronomical practices – including microclimate modification (e.g. sunlight incidence or temperature), water availability and irrigation, and postharvest management – affect fruit development and composition. These studies report well-settled transcriptomic and metabolomic methods, in addition to newly-developed techniques addressing proteome profiles, genome methylation landscapes and ionomic signatures, some of which attempt to tackle the influence of terroir, i.e. the synergic effect of (micro)climate, soil composition, grape genotype, and vineyard practices. A few reviews and opinions are included that focus on the advantages of applying network theory in grapevine research. Studies on vegetative organs in their relation to fruit development and on fruit-derived cell cultures are also considered.
This book illustrates experimental and computational methodologies used to achieve cost effective biological processes for the production of fuels and biochemicals through multiple approaches to increasing yield, titers, and productivity in a robust host. The volume includes the most recent and cutting-edge aspects of pathway engineering, flux analysis, and metabolic enzyme engineering. Each chapter highlights the complexity and challenges of the problem as well as the methods used to solve this problem or changes needed in current methods. As a part of the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that gives researchers a much needed boost. Authoritative and practical, Metabolic Pathway Engineering benefits not only scientists working on more fundamental aspects of this endeavor but also those in the biochemical industry working on strain engineering for robust industrial processes.