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This paper provides guidelines for new high-throughput screening methods – both phenotypic and genotypic – to enable the detection of rare mutant traits, and reviews techniques for increasing the efficiency of crop mutation breeding.
The book covers information on various types of mutagens and their effects, procedures for using mutagens for crop improvement, types of mutations (micro and macro) with statistical techniques to handle the mutation population. The subject matter presented in the book will be useful for both undergraduate and post graduate students of agriculture.
This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.
Abstract: This book presents contemporary information on mutagenesis in plants and its applications in plant breeding and research. The topics are classified into sections focusing on the concepts, historical development and genetic basis of plant mutation breeding (chapters 1-6); mutagens and induced mutagenesis (chapters 7-13); mutation induction and mutant development (chapters 14-23); mutation breeding (chapters 24-34); or mutations in functional genomics (chapters 35-41). This book is an essential reference for those who are conducting research on mutagenesis as an approach to improving or modifying a trait, or achieving basic understanding of a pathway for a trait --.
An essential and comprehensive summary for all plant breeders.
Plant breeding is estimated to have begun 9,000 11,000 years ago and mutation breeding is simply a new device in the breeders toolkit. Mutation breeding has been used since the 1930s. Mutation breeding, sometimes referred to as "variation breeding", is the process of exposing seeds to chemicals or radiation in order to generate mutants with desirable traits to be bred with other cultivars. Plants created using mutagenesis are sometimes called mutagenic plants or mutagenic seeds. Normal breeding involves cross breeding, from the parents, through a multi-generational process that may take four to five years to eliminate an unwanted genome and develop the sought-after traits before trial crops are tested, adding more time. Mutation breeding is based on selfing mutants until the induced character has a stable expression in the advanced mutant generations. Mutation breeding has many comparative advantages. It is cost effective, quick, proven and robust. In addition, mutation breeding is transferrable, ubiquitously applicable, non-hazardous and environmentally friendly. There are different kinds of mutagenic breeding such as using chemical mutagens like EMS and DMS, radiation and transposons are used to generate mutants. Mutation breeding is commonly used to produce traits in crops such as larger seeds, new colors, or sweeter fruits that either cannot be found in nature or have been lost during evolution. The book Basics of Mutation Breeding covers information on various types of mutagens and their effects, procedures for using mutagens for crop improvement, types of mutations (micro and macro) with statistical techniques to handle the mutation population. The book will be useful for both undergraduate and post graduate students of agriculture.
Genetic diversity is one of the measures of biodiversity and has consequences in biological variation. It is crucial to understand the evolutionary and adaptative processes in all living species. This book is an interdisciplinary and integrated work that will contribute to the knowledge of academics from different areas of biological sciences. This collection of scientific papers was chosen and analyzed to offer readers a broad and integrated view of the importance of genetic diversity in the evolution and adaptation of living beings, as well as practical applications of the information needed to analyze this diversity in different organisms. This book was edited by geneticist researchers and provides academics with up-to-date and quality information on the subject.
Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.
In the present era various international organizations, such as FAO, UNO, IAEA, FNCA, etc., have unanimously agreed that millions of people in both developing and developed countries are not only facing a shortage of food, but also non-availability of nutrients. The main reason put forward by these agencies is that there is less genetic diversity prevalent in the major crops, which has been further diminished since the inception of conventional plant breeding. Since the first decade of the last century the mutation breeding approach has been pivotal in enhancing the genetic diversity of crops, thereby enriching the genetic pool. `Mutagenesis: exploring genetic diversity of crops¿ describes the latest achievements in mutation breeding, with a particular focus on the development of novel mutant varieties and F1 hybrids of crops highly superior to the parental ones. The book details experimental as well as literary studies of induced mutagenesis and its role in developing the new potent varieties. The book will be useful for agricultural policy making authorities in countries of agricultural importance, scientific researchers, breeders, teachers and students keen to use mutation breeding and to explore its hidden potential to secure food and nutrient availability for the growing world population.