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This book is a practical guidebook in biochemistry, for medical as well as life sciences' students. The book covers reference values, sample collection procedure and detailed protocol to perform experiments. Each experiment starts with a brief introduction of the protocol, followed by specimen requirements and procedure. The procedures are presented in a very lucid manner and discuss details of calculations and clinical interpretations, The book is divided into 29 chapters, It offers references, general guidelines and abbreviations and provides principles and procedures of clinical biochemistry tests, along with their diagnostic importance.
This book is a practical guidebook in biochemistry, for medical as well as life sciences' students. The book covers reference values, sample collection procedure and detailed protocol to perform experiments. Each experiment starts with a brief introduction of the protocol, followed by specimen requirements and procedure. The procedures are presented in a very lucid manner and discuss details of calculations and clinical interpretations,The book is divided into 29 chapters, It offers references, general guidelines and abbreviations and provides principles and procedures of clinical biochemistry tests, along with their diagnostic importance.
"Basic Concepts in Biochemistry has just one goal: to review the toughest concepts in biochemistry in an accessible format so your understanding is through and complete."--BOOK JACKET.
Finding a simple and step-by-step procedure to conduct clinical biochemistry-related analyses is a real challenge for many undergraduate, graduate students, researchers and technicians in universities and laboratories. Moreover, understanding the theory of the experiment, which is not provided in some currently available manuals, is a useful and essential requirement in the experiment for successful performance, accuracy and acceptable results. The book contains 14 chapters. The first three chapters describe essential clinical aspects in laboratory such as specimens used for clinical chemistry analysis and sample collecting methods with common sampling errors. In addition, the fundamentals and laboratory techniques commonly used for sample analysis such as centrifugation, electrophoresis, photometry, fluorometry, and chromatography are also covered in one separate chapter. The later chapters discuss the biologic basics of liver, kidney and heart diseases and the common enzymes measured to assess the function of these organs. Moreover, properties, diagnosis and analysis of vital minerals disorders such as iron, calcium, phosphate, zinc and magnesium are discussed in five different chapters. Hematological disorders related to nutrition and some case histories and comments are added in order to help students to analyze and interpret the lab results in proper way. The book also has a separate chapter with lot of case studies and their solutions for better understanding. This book will be a useful reference for new students, non-native English medicine and life science students as it relies on figures and diagrams that explain the concepts and diagnosis of diseases in a simple way. Therefore, this quick guide aims to provide and develop the basic practical skills in the users with simple steps to follow along with the theoretical explanation for better understanding. It is expected that this quick handbook will provide good tools and useful guidelines for the students and researchers as well.
Protocols in Biochemistry and Clinical Biochemistry, second edition, offers clear, applied instruction in fundamental biochemistry methods and protocols, from buffer preparation to nucleic acid purification, protein, lipid, carbohydrate, and enzyme testing, and clinical testing of vitamins, glucose, and cholesterol levels, among other diagnostics. Each protocol is illustrated with step-by-step instructions, labeled diagrams, and color images, as well as a thorough overview of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods, and troubleshooting, all to support a range of study types and clinical diagnostics. This fully revised edition has been expanded and enriched to feature 100 protocols, as well as chapter key term definitions and worked examples. All-new protocols added to this edition include identification of lipids by TLC, lipid per oxidation measurement by thiobarbituric acid assays, determination of serum amylase, catalase activity assay, superoxide dismutase assay, qualitative analysis of plant secondary metabolites, qualitative analysis of photochemicals, quantitative estimation of secondary metabolites, estimation of chlorophyll contents, and starch determination, among others. Each protocol is written to help researchers and clinicians easily reproduce lab methods and ensure accurate test results. - Includes full listings and discussions of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods, and troubleshooting across 100 protocols - Features clear, step-by-step instruction with color diagrams and images, followed by worked examples of putting lab techniques into action - Empowers researchers and clinicians to reproduce research and clinical methods and ensure test accuracy
Contemporary Practice in Clinical Chemistry, Fourth Edition, provides a clear and concise overview of important topics in the field. This new edition is useful for students, residents and fellows in clinical chemistry and pathology, presenting an introduction and overview of the field to assist readers as they in review and prepare for board certification examinations. For new medical technologists, the book provides context for understanding the clinical utility of tests that they perform or use in other areas in the clinical laboratory. For experienced laboratorians, this revision continues to provide an opportunity for exposure to more recent trends and developments in clinical chemistry. - Includes enhanced illustration and new and revised color figures - Provides improved self-assessment questions and end-of-chapter assessment questions
We are very pleased to put forth the revised edition of 'Laboratory Manual of Biochemistry and Clinical Pathology'. We have incorporated all the suggestions, modified it to make it easier, student friendly and relevant in terms of achieving curriculum outcome. We are very much thankful to all the learned teachers who have given their feedback whole-heartedly. We have even incorporated the changes in this manual based on the feedback given by the teachers from all the institutes. Now, we believe that the manual has been fulfilling the aspirations of biochemistry teachers and students too. This manual is prepared as per PCI Education Regulations, 2020 for Diploma Course in Pharmacy. The methods of all the experiments are reviewed and added from the recent research papers, so that the advancement in the methods or apparatus can be addressed. This manual is designed for 'outcome-based education' and each experiment is arranged in a uniform way such as practical significance, practical outcomes (PrOs) and its mapping with course outcomes, minimum theoretical background, resources used, procedure, precautions, observations, result, conclusion, references and related questions. Moreover, assessment scheme is also given to help the student and teacher to know what to be assessed. During the laboratory period, you will have to multitask, while you are doing the experiment. It is essential to document properly what you do and what you observe while doing the practical. Always plan your work ahead and think about what you are doing, why you are doing it, what is happening, and what you can conclude from your experiment.
This textbook explains the basic principles of Biochemistry, Nutrition and Dietetics and their application to health and disease. It presents core information to introduce basic concepts and thereby apply the acquired knowledge in nursing practice. Third edition is comprehensively updated to meet the constantly changing health needs of people. Content has been reorganized and significant changes have been made during the development of the text to include addition of a new section on biochemistry and recent updates in the Nutrition section as per the revised syllabus outlined by the Indian Nursing Council. This book can be used by students and teachers of Biochemistry, Nutrition, Dietetics, Nursing, Medicine, and other health sciences. Highlights: Now in FULL COLOR! UPDATED! As per the revised Indian Nursing Council syllabus NEW! Section on biochemistry comprising 8 chapters “Nutrition” included in chapter Therapeutic Diets to address the basic nutrition needs of affected patients NEW! Chapter Nutrition Deficiency Disorders included which covers causes, signs and symptoms, and management of important and prevalent disease conditions such as severe acute malnutrition, childhood obesity, and deficiency disorders of vitamins and minerals UPDATED! Recommended dietary allowances, IYCF guidelines, anemia in pregnancy and adolescence, and nutrition education Recipes for different types of diet and sample menus for important diseases included for ready reference Important topics like “Calculation of nutritive value of foods” included with examples for easy understanding Enzymes of diagnostic importance for various diseases discussed Metabolism of carbohydrates, proteins, and lipids illustrated for better understanding Content presented in a student friendly manner complemented with plenty of illustrations, flowcharts, and tables Chapter-end summaries for quick review and Self-Assessment section as per University examination pattern An extensive glossary included.
We are pleased to put forth the "Laboratory Manual of Biochemistry." This manual, prepared according to the PCI B. Pharm course regulations 2014, is divided into four sections: qualitative analysis, quantitative analysis, estimation of blood parameters and catalytic role of enzymes. The methods of all the experiments are drawn from the latest editions of official books such as the Indian Pharmacopoeia and research papers, ensuring the inclusion of the latest advancements in methodologies or apparatus. This manual is designed for outcome-based education. Each experiment follows a uniform format, with sections for practical significance, practical outcomes (PrOs), mapping with course outcomes, theory, resources used, procedure, precautions, observations, results, conclusion, references, and synopsis questions. Each experiment offers an opportunity for students to perform practical work, developing proficiency in effectively managing equipment, handling glassware, chemicals, reagents, and writing analytical reports. In addition, the questions at the end of the experiments help to enhance students' knowledge, benefiting them as they pursue higher studies. During the laboratory period, you will need to juggle multiple tasks while performing the experiment. It is essential to document your actions and observations thoroughly as you proceed. Always plan your work ahead, considering what you are doing, why you are doing it, what is happening, and what conclusions you can draw from your experiment. We acknowledge the help and cooperation of various individuals in bringing out this manual. We are highly indebted to the authors of the books and articles mentioned in the references, which were a major source of information for this manual. We also thank the publishers, designers, and printers who worked hard to publish this manual in a timely manner. We hope that this manual will be helpful to students in understanding concepts, principles, and performing procedures. We wish you all the best!.
"It has been 5 years since the fifth edition was completed. The sixth edition has some significant organizational changes, as suggested by extensive surveys of faculty and students who used the fifth edition in their classes and studies"--