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This volume explores the various methods used to study tertiary lymphoid structures (TLS) in pathological situations. Pre-clinical models are also discussed in detail to show how TLS structure, development, and maintenance can be targeted and studied in vivo. The chapters in this book cover topics such as humans and mice; strategies to quantify TLS in order to use it in stained tissue sections; classifying a gene signature form fixed and paraffin-embedded tissues; and development of murine inflammatory models to help look at TLS in the context of infection or malignancy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Tertiary Lymphoid Structures: Methods and Protocols is a valuable resource that increases the reader’s knowledge on immune functions and how they will pave the way to future therapeutic applications.
This text is designed to present a comprehensive, up to date and safe approach to managing emergency general surgery patients with a minimally invasive approach. This book bridges the gap between the minimally invasive surgery (MIS) expert who may not routinely be involved in the care of the acute care surgery patient and the ACS expert surgeon who may not have a routine MIS elective practice. The first section reviews the physiology of the acute care surgery patient and the physiologic impact of MIS techniques including pnemoperitoneum in critically ill patients. This review is crucial to assure that the surgeon has a clear understanding of when a MIS approach is safe and when it can be potentially dangerous to the patient. The illustrations are presented in an atlas-style format to describe the surgical procedures. The authors of the various sections are minimally invasive and acute care surgeons from prominent academic centers. The format represents a narrative review with appropriate illustrations and links to online videos.
This text provides a comprehensive textbook summarizing the presentation, workup, and surgical solutions for common emergencies in the cancer patient. The use of medications (antibiotics, hormones, and biologic therapies) and interventional radiologic procedures as adjuncts or as replacements for the surgical solutions are discussed. The text covers emergencies that result as adverse effects for the systemic and local treatments for cancer, emergencies that result from mechanical issues with the cancer and those that result from surviving major extirpative surgery. Diagnosis and treatment of patients' immune-compromised or thrombocytopenic status from chemotherapy is discussed, as is surgical treatment in patients with prior complex surgical therapy or radiation treatment. Finally, the text covers alternatives to surgery, including new interventional radiologic and endoscopic procedures. Surgical Emergencies in the Cancer Patient will be of great value to healthcare professionals at all levels who are involved in the treatment of emergencies in the cancer patient. It is meant for a wide group of individuals, including medical students, residents in surgery and internal medicine, fellows in surgery and oncology, and practicing oncologists and surgeons. This work is meant to be a textbook for the student, a guide for the practitioner, and a reference for the general oncologist.
Adapted from the Charpentier "Te Deum in D Major" with an original school-friendly text, this is an accessible and positive way to ease your students into singing timeless choral music. An optional trumpet adds to the classic character. Majestic!
Acute Care General Surgery provides a succinct focused overview of a myriad of general surgical emergencies. Each chapter has a concise, practical framework on the workup and management of a specific topic. The book covers a wide array of pathology from the more common appendicitis, diverticulitis and cholecystitis to those less frequently encountered such as toxic megacolon, Fournier's gangrene and volvulus. Learning objectives for each chapter are clearly defined. In addition to differential diagnosis, the work up and management is comprehensively discussed. In addition, a pithy clinical pearl is included to serve as a memory aid for each diagnosis. Upon reviewing this volume, clinicians who encounter the acute care surgical patient in their practices will be better equipped to rapidly assess, evaluate, diagnose and managing their complex and often challenging problems.