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This handbook covers the most commonly used techniques for measuring plant response to biotic and abiotic stressing factors, including: in vitro and in vivo bioassays; the study of root morphology, photosynthesis (pigment content, net photosynthesis, respiration, fluorescence and thermoluminiscence) and water status; thermal imaging; the measurement of oxidative stress markers; flow cytometry for measuring cell cycle and other physiological parameters; the use of microscope techniques for studying plant microtubules; programmed-cell-death; last-generation techniques (metabolomics, proteomics, SAR/QSAR); hybridization methods; isotope techniques for plant and soil studies; and the measurement of detoxification pathways, volatiles, soil microorganisms, and computational biology.
Brings together plant ecophysiology, remote sensing and modelling of vegetation and landscape function for advanced students and researchers.
The Handbook of Plant Ecophysiology Techniques you have now in your hands is the result of several combined events and efforts. The birth of this handbook can be traced as far as 1997, when our Plant Ecophysiology lab at the University of Vigo hosted a practical course on Plant Ecophysiology Techniques. That course showed us how much useful a handbook presenting a bunch of techniques would be for the scientists beginning to work on Plant Ecophysiology. In fact, we wrote a short handbook explaining the basics of the techniques taught in that 1997 course: Flow cytometry to measure ploidy levels, Use of a Steady-State porometer to measure transpiration, In vivo measure of fluorescence, HPLC analysis of low molecular weight phenolics, Spectrophotometric determinations of free proline and soluble proteins, TLC polyamines contents measures, Isoenzymatic electrophoresis, Use of IRGA and oxygen electrode. That modest handbook, written in Spanish, was very helpful, both for the people who attended the course and for other who have used it for beginning to work in Plant Ecophysiology. The present Handbook is much more ambitious, and it includes more techniques. But we have also had in mind the young scientists beginning to work on Plant Ecophysiology. In 1999 François Pellissier leaded a proposal presented to the European Commission in the Fifth Framework Program in the High Level * Scientific Conferences, including three EuroLab Courses about lab and field techniques useful to improve allelopathic research.
Microbes are ubiquitous in nature, and plant-microbe interactions are a key strategy for colonizing diverse habitats. The plant microbiome (epiphytic, endophytic and rhizospheric) plays an important role in plant growth and development and soil health. Further, rhizospheric soil is a valuable natural resource, hosting hotspots of microbes, and is vital in the maintenance of global nutrient balance and ecosystem function. The term endophytic microbes refers to those microorganisms that colonize the interior the plants. The phyllosphere is a common niche for synergism between microbes and plants and includes the leaf surface. The diverse group of microbes are key components of soil-plant systems, and where they are engaged in an extensive network of interactions in the rhizosphere/endophytic/phyllospheric they have emerged as an important and promising tool for sustainable agriculture. Plant microbiomes help to directly or indirectly promote plant growth using plant growth promoting attributes, and could potentially be used as biofertilizers/bioinoculants in place of chemical fertilizers. This book allows readers to gain an understanding of microbial diversity associated with plant systems and their role in plant growth, and soil health. Offering an overview of the state of the art in plant microbiomes and their potential biotechnological applications in agriculture and allied sectors, it is a valuable resource for scientists, researchers and students in the field of microbiology, biotechnology, agriculture, molecular biology, environmental biology and related subjects.
Plants in tropical regions are coping with enormous challenges of physiological stresses owing to changing environmental and climatic conditions. Rapid growth of human population and rampant exploitation of fossil fuels and other developmental activities are actively contributing to such perturbations. The Intergovernmental Panel on Climate Change has projected a sustained increase in carbon dioxide (CO2) emissions and thereby a rise in global temperature in the coming decades. The resultant changes in precipitation patterns are now evident across the globe due to intensication of hydrological cycle. Moreover, gaseous and particulate pollutants are also an immense challenge for tropical plants. Such vagaries in environmental conditions have signicant impacts on the ecophysiological traits of plants, resulting from altered interactions of tropical plants with each other, as well as other biotic and abiotic components within the ecosystem. Books available in the market that particularly focus on ecophysiological responses of tropical plants to abiotic and biotic environmental factors under climate change are limited. This book intends to fill this knowledge gap and provides a detailed analysis on ecophysiological responses of tropical plants to these environmental challenges, as well as suggesting some approachable measures for plant adaptations to these challenges. The book is equally applicable to undergraduate and postgraduate students, researchers, teachers and forest managers, and policy makers. Salient features of the book are: 1. A comprehensive discussion on adaptive mechanisms of plants through their ecophysiological responses to various biotic and abiotic stresses. 2. Elaboration on the recent techniques involved in ecophysiological research. 3. A detailed account of evolutionary responses of plants to changing climate. 4. Discussion of recent research results and some pointers to future advancements in ecophysiological research. 5. Presentation of information in a way that is accessible for students, researchers, and teachers practicing in plant physiology and ecology.
Conifers include a wide range of species that are spread all over the world. These species have wide diversity, variable stand structures ranging from monospecific monocohort to multispecific multicohort, and produce an assortment of products and services, the most frequent of which is timber. This book is a collection of contributions, both reviews and research studies, from different fields and perspectives on the management, regeneration, growth, diversity, and production of conifer stands. The book also addresses the effect of wildfires on conifer ecosystems and respiratory allergies to conifers.
Twenty-nine, prominent, international researchers provide contributions which deal with understanding the basic ecophysiological and molecular principles governing the functioning of plant systems in relation to their environment. Divided into two headings: biotic and abiotic; the first consists of abiotic, natural environmental factors--light, ultraviolet radiation, chilling and freezing, high temperatures, drought, flooding, salt and trace metals. The latter half presents anthropogenic aspects including allelochemicals, herbicides, polyamines, air pollutants, carbon dioxide, radioisotopes and fire.
Plant Life under Changing Environment: Responses and Management presents the latest insights, reflecting the significant progress that has been made in understanding plant responses to various changing environmental impacts, as well as strategies for alleviating their adverse effects, including abiotic stresses. Growing from a focus on plants and their ability to respond, adapt, and survive, Plant Life under Changing Environment: Responses and Management addresses options for mitigating those responses to ensure maximum health and growth. Researchers and advanced students in environmental sciences, plant ecophysiology, biochemistry, molecular biology, nano-pollution climate change, and soil pollution will find this an important foundational resource. - Covers both responses and adaptation of plants to altered environmental states - Illustrates the current impact of climate change on plant productivity, along with mitigation strategies - Includes transcriptomic, proteomic, metabolomic and ionomic approaches
Chlorophyll a Fluorescence: A Signature of Photosynthesis highlights chlorophyll (Chl) a fluorescence as a convenient, non-invasive, highly sensitive, rapid and quantitative probe of oxygenic photosynthesis. Thirty-one chapters, authored by 58 international experts, provide a solid foundation of the basic theory, as well as of the application of the rich information contained in the Chl a fluorescence signal as it relates to photosynthesis and plant productivity. Although the primary photochemical reactions of photosynthesis are highly efficient, a small fraction of absorbed photons escapes as Chl fluorescence, and this fraction varies with metabolic state, providing a basis for monitoring quantitatively various processes of photosynthesis. The book explains the mechanisms with which plants defend themselves against environmental stresses (excessive light, extreme temperatures, drought, hyper-osmolarity, heavy metals and UV). It also includes discussion on fluorescence imaging of leaves and cells and the remote sensing of Chl fluorescence from terrestrial, airborne, and satellite bases. The book is intended for use by graduate students, beginning researchers and advanced undergraduates in the areas of integrative plant biology, cellular and molecular biology, plant biology, biochemistry, biophysics, plant physiology, global ecology and agriculture.
Since the publication of the third edition of the Handbook of Plant and Crop Stress, continuous discoveries in the fields of plant and crop environmental stresses and their effects on plants and crops have resulted in the compilation of a large volume of the latest discoveries. Following its predecessors, this fourth edition offers a unique and comprehensive collection of topics in the fields of plant and crop stress. This new edition contains more than 80% new material, and the remaining 20% has been updated and revised substantially. This volume presents 10 comprehensive sections that include information on soil salinity and sodicity problems; tolerance mechanisms and stressful conditions; plant/crop responses; plant/crop responses under pollution and heavy metal; plant/crop responses under biotic stress; genetic factors and plant/crop genomics under stress conditions; plant/crop breeding under stress conditions; empirical investigations; improving tolerance; and beneficial aspects of stressors. Features: Provides exhaustive coverage written by an international panel of experts in the field of agriculture, particularly in plant/crop stress areas Contains 40 new chapters and 10 extensively revised and expanded chapters Includes three new sections on plant breeding, stress exerted to weeds by plants, and beneficial aspects of stress on plants/crops Numerous case studies With contributions from 100 scientists and experts from 20 countries, this Handbook provides a comprehensive resource for research and for university courses, covering soil salinity/sodicity issues and plant/crop physiological responses under environmental stress conditions ranging from cellular aspects to whole plants. The content can be used to plan, implement, and evaluate strategies to mitigate plant/crop stress problems. This new edition includes numerous tables, figures, and illustrations to facilitate comprehension of the material as well as thousands of index words to further increase accessibility to the desired information.