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Whilst assisted reproduction techniques (ART) have become increasingly successful and largely standardized, there is still only a partial understanding of what constitutes a 'true' embryo environment. Replicating the varying physiological conditions of the in-vivo environment that the embryo travels through in the in-vitro culture is still a major challenge in ART. This practical volume details how to organize and operate an IVF laboratory in order to mimic these conditions for successful embryo culture. Environments and equipment that are essential for running safe and efficient facilities such as maintaining good air quality and hygiene protocols, and utilizing an effective layout are covered in detail. Other chapters discuss the different consumables needed, optimal handling techniques and parameter monitoring systems, as well as recent advances in the area including artificial intelligence and automation. This is an indispensable guide to understanding the background science of culturing embryos, crucial to successful outcomes in ART.
Being human is hard. Being a good human is even harder. Practicing kindness, honesty, and self-awareness in the face of doubt, failure, ambiguity, and vulnerability can feel insurmountable. How to Human is here to help. Alice Connor draws on nearly a decade of experience as a college chaplain to provide a tender and irreverent take on one of life's most fundamental questions: how to be a better human in a world dead set against it. Connor offers sage wisdom and no-nonsense realism through real-life examples that strike right at the rashes and rubs of the human experience. She'll take you by the hand, tell you what you need to hear, and encourage you to embrace the chaos. How to Human will help you see life as an experiment--not a quest for the right answers.
This manual is a comprehensive compilation of "methods that work" for deriving, characterizing, and differentiating hPSCs, written by the researchers who developed and tested the methods and use them every day in their laboratories. The manual is much more than a collection of recipes; it is intended to spark the interest of scientists in areas of stem cell biology that they may not have considered to be important to their work. The second edition of the Human Stem Cell Manual is an extraordinary laboratory guide for both experienced stem cell researchers and those just beginning to use stem cells in their work. - Offers a comprehensive guide for medical and biology researchers who want to use stem cells for basic research, disease modeling, drug development, and cell therapy applications - Provides a cohesive global view of the current state of stem cell research, with chapters written by pioneering stem cell researchers in Asia, Europe, and North America - Includes new chapters devoted to recently developed methods, such as iPSC technology, written by the scientists who made these breakthroughs
Charles Darwin changed the course of scientific thinking by showing how evolution accounts for the stunning diversity and biological complexity of life on earth. Recently, there has also been increased interest in the social sciences in how Darwinian theory can explain human culture. Covering a wide range of topics, including fads, public policy, the spread of religion, and herd behavior in markets, Alex Mesoudi shows that human culture is itself an evolutionary process that exhibits the key Darwinian mechanisms of variation, competition, and inheritance. This cross-disciplinary volume focuses on the ways cultural phenomena can be studied scientifically—from theoretical modeling to lab experiments, archaeological fieldwork to ethnographic studies—and shows how apparently disparate methods can complement one another to the mutual benefit of the various social science disciplines. Along the way, the book reveals how new insights arise from looking at culture from an evolutionary angle. Cultural Evolution provides a thought-provoking argument that Darwinian evolutionary theory can both unify different branches of inquiry and enhance understanding of human behavior.
Anthropology is the study of all humans in all times in all places. But it is so much more than that. "Anthropology requires strength, valor, and courage," Nancy Scheper-Hughes noted. "Pierre Bourdieu called anthropology a combat sport, an extreme sport as well as a tough and rigorous discipline. ... It teaches students not to be afraid of getting one's hands dirty, to get down in the dirt, and to commit yourself, body and mind. Susan Sontag called anthropology a "heroic" profession." What is the payoff for this heroic journey? You will find ideas that can carry you across rivers of doubt and over mountains of fear to find the the light and life of places forgotten. Real anthropology cannot be contained in a book. You have to go out and feel the world's jagged edges, wipe its dust from your brow, and at times, leave your blood in its soil. In this unique book, Dr. Michael Wesch shares many of his own adventures of being an anthropologist and what the science of human beings can tell us about the art of being human. This special first draft edition is a loose framework for more and more complete future chapters and writings. It serves as a companion to anth101.com, a free and open resource for instructors of cultural anthropology. This 2018 text is a revision of the "first draft edition" from 2017 and includes 7 new chapters.
Humans are a striking anomaly in the natural world. While we are similar to other mammals in many ways, our behavior sets us apart. Our unparalleled ability to adapt has allowed us to occupy virtually every habitat on earth using an incredible variety of tools and subsistence techniques. Our societies are larger, more complex, and more cooperative than any other mammal's. In this stunning exploration of human adaptation, Peter J. Richerson and Robert Boyd argue that only a Darwinian theory of cultural evolution can explain these unique characteristics. Not by Genes Alone offers a radical interpretation of human evolution, arguing that our ecological dominance and our singular social systems stem from a psychology uniquely adapted to create complex culture. Richerson and Boyd illustrate here that culture is neither superorganic nor the handmaiden of the genes. Rather, it is essential to human adaptation, as much a part of human biology as bipedal locomotion. Drawing on work in the fields of anthropology, political science, sociology, and economics—and building their case with such fascinating examples as kayaks, corporations, clever knots, and yams that require twelve men to carry them—Richerson and Boyd convincingly demonstrate that culture and biology are inextricably linked, and they show us how to think about their interaction in a way that yields a richer understanding of human nature. In abandoning the nature-versus-nurture debate as fundamentally misconceived, Not by Genes Alone is a truly original and groundbreaking theory of the role of culture in evolution and a book to be reckoned with for generations to come. “I continue to be surprised by the number of educated people (many of them biologists) who think that offering explanations for human behavior in terms of culture somehow disproves the suggestion that human behavior can be explained in Darwinian evolutionary terms. Fortunately, we now have a book to which they may be directed for enlightenment . . . . It is a book full of good sense and the kinds of intellectual rigor and clarity of writing that we have come to expect from the Boyd/Richerson stable.”—Robin Dunbar, Nature “Not by Genes Alone is a valuable and very readable synthesis of a still embryonic but very important subject straddling the sciences and humanities.”—E. O. Wilson, Harvard University
How do biological, psychological, sociological, and cultural factors combine to change societies over the long run? Boyd and Richerson explore how genetic and cultural factors interact, under the influence of evolutionary forces, to produce the diversity we see in human cultures. Using methods developed by population biologists, they propose a theory of cultural evolution that is an original and fair-minded alternative to the sociobiology debate.
This manual is designed to serve as a practical guide to primary human cell culture, which is integral in both academic and industrial biotechnology research. As in the first edition, the content of the manual is not exhaustive, but rather contains selected protocols for specific cell types from major tissue groupings in the body. This improved second edition also includes a new section on stem cells and additional material on transfection. It should serve as a foundation for individual researchers to experiment, explore, and establish niche protocols for their specific needs. With its compact physical format that makes it portable and flexible for usage in a laboratory setting, the manual will be a useful guide for all beginners in primary human cell culture work.
How our collective intelligence has helped us to evolve and prosper Humans are a puzzling species. On the one hand, we struggle to survive on our own in the wild, often failing to overcome even basic challenges, like obtaining food, building shelters, or avoiding predators. On the other hand, human groups have produced ingenious technologies, sophisticated languages, and complex institutions that have permitted us to successfully expand into a vast range of diverse environments. What has enabled us to dominate the globe, more than any other species, while remaining virtually helpless as lone individuals? This book shows that the secret of our success lies not in our innate intelligence, but in our collective brains—on the ability of human groups to socially interconnect and learn from one another over generations. Drawing insights from lost European explorers, clever chimpanzees, mobile hunter-gatherers, neuroscientific findings, ancient bones, and the human genome, Joseph Henrich demonstrates how our collective brains have propelled our species' genetic evolution and shaped our biology. Our early capacities for learning from others produced many cultural innovations, such as fire, cooking, water containers, plant knowledge, and projectile weapons, which in turn drove the expansion of our brains and altered our physiology, anatomy, and psychology in crucial ways. Later on, some collective brains generated and recombined powerful concepts, such as the lever, wheel, screw, and writing, while also creating the institutions that continue to alter our motivations and perceptions. Henrich shows how our genetics and biology are inextricably interwoven with cultural evolution, and how culture-gene interactions launched our species on an extraordinary evolutionary trajectory. Tracking clues from our ancient past to the present, The Secret of Our Success explores how the evolution of both our cultural and social natures produce a collective intelligence that explains both our species' immense success and the origins of human uniqueness.