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Monoterpene composition was studied in a number of hard pine species and results were compared with earlier work. (I) Intratree measurements showed strong constancy of composition in both single-stemmed and forked trees of ponderosa, Jeffrey, Coulter, and Jeffrey x ponderosa pines. In grafts of these and other pines, the scion influenced the root stock, but not the reverse. (2) Large intcrtree variation in composition was found in a small sample of seven hard pines native to Mexico; the value of monoterpenes in clarifying taxonomy of these pines remains uncertain. (3) An 80-tree plot of ponderosa is adequate to determine average monoterpene composition. For the best estimate of the kinds and abundance of types of composition, a 350-tree plot is needed. Short-column chromatographic analysis is acceptably accurate for rapid classification of a large number of samples.
The present volume contains papers developed from courses given at the International Union of Forest Research Organizations (IUFRO) Bio chemical Genetics Workshop (Working Party S.04-5) held at the Univer sity of Gottingen, Germany on July 5 through 28, 1973. The workshop was organized by Professor Robert G. Stanley and was held in memory of Professor Klaus Stern. Unfortunately, both met with untimely deaths. Professor Stanley was also instrumental in initiating the process of having the workshop proceedings published. I was asked by the workshop participants to complete this task, and I wish to acknowledge their cooperation, advice and encouragement. In addition to the courses and subsequent papers resulting from the above workshop, we have included some papers by colleagues who were unable to attend the meeting. The contents of this text may, there fore, be considered a working-manual of generally "modern" techniques that are applicable to forest genetics and breeding programs. The chapters are placed in five major categories. The first three categories follow according to classes of chemical constituents in herent to plants which are nucleic acids (DNA, RNA) , primary gene products (amino acids, proteins and enzymes) and primary and secon dary metabolites (carbohydrate polymers, resins, phenolics, pigments, etc.). The fourth category is concerned with the interaction of en vironment and gene systems. Indirect selection, crossing and proto plasmic and flowering manipulation are factors covered in the fifth category.