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This book, which was originally published in 1986, introduces the reader to the main steps in the analysis of chimeras, explains their structural and developmental basis, and the ways of classifying and manipulating them. The twelve chapters separate types of chimeras according to their origin - by grafting or polyploidy; their structure - sectorial or periclinal; or according to the varied parts of the plant most affected - tubers, leaves, flowers or fruit. Throughout the book care is taken to distinguish between the activity of the growing-point in determining chimera structure, and the role of gene expression in determining appearance. Examples of the experimental uses of chimeras are given and of the valuable role they can play in studying fundamental questions of anatomical development; the disadvantages of chimeras in mutation breeding are discussed too.
This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.
Grow in the Dark puts the spotlight on 50 of the best houseplants you can grow in your dim or dark apartment. Author Lisa Eldred-Steinkopf, known as the Houseplant Guru, shares the knowledge she’s gained tending to her own personal jungle of over 1,000 houseplants. Having a south-facing window doesn’t always guarantee you the best light to grow plants—especially if your window faces an alley or a tree-lined street. What’s the point of growing an urban jungle if tall buildings are blocking all your sunshine? This compact guide, designed to look as good on your shelf as it is useful, will help you learn how to make the most of your light so you can reap the physical and emotional benefits of living with plants. Detailed profiles include tips on watering your plants just right, properly potting them, and troubleshooting pests and diseases. You’ll also learn which plants are safe to keep around your pets. Whether you live in a shady top-floor apartment or a dungeon-y garden level, this book will help you grow your plant collection to its healthiest for its Instagram debut.
This paper provides guidelines for new high-throughput screening methods – both phenotypic and genotypic – to enable the detection of rare mutant traits, and reviews techniques for increasing the efficiency of crop mutation breeding.
From New York Times bestselling author Mira Grant comes the final book in the terrifying Parasitology series. The outbreak has spread, tearing apart the foundations of society, as implanted tapeworms have turned their human hosts into a seemingly mindless mob. Sal and her family are trapped between bad and worse, and must find a way to compromise between the two sides of their nature before the battle becomes large enough to destroy humanity, and everything that humanity has built. . . including the chimera. The broken doors are closing. Can Sal make it home? "A riveting near-future medical thriller that reads like the genetically-engineered love child of Robin Cook and Michael Crichton." —John Joseph Adams on Parasite More from Mira Grant: Parasitology Parasite Symbiont Chimera Newsflesh Feed Deadline Blackout Feedback Rise
Survey of literature on mutation in potato; Production of a diphoid potato clone with genetic markers; Formation of adventitious roots and shoots from potato leaves, leaf parts and stems in vitro; Literature on organization, post-irradiation behaviour and histogenic effects in shoot apices; Radiation-induced-damage and recovery of potato tuber eyers.
Abstract: This book presents contemporary information on mutagenesis in plants and its applications in plant breeding and research. The topics are classified into sections focusing on the concepts, historical development and genetic basis of plant mutation breeding (chapters 1-6); mutagens and induced mutagenesis (chapters 7-13); mutation induction and mutant development (chapters 14-23); mutation breeding (chapters 24-34); or mutations in functional genomics (chapters 35-41). This book is an essential reference for those who are conducting research on mutagenesis as an approach to improving or modifying a trait, or achieving basic understanding of a pathway for a trait --.
This volume addresses challenging new questions surrounding stem cell-based chimera research. This book is organized into three parts: Part One provides readers with a summary of different human donor cell types. The chapters in this section discuss ways to evaluate new types of pluripotent stem cells; the derivation of naïve and primed pluripotent stem cells from mouse preimplantation embryos; and the ethical and regulatory complexities of informed consent for the procurement of somatic cells. Part Two discusses methods for generating chimeras. The chapters here look at chick models and human-chick organizer grafts; generating human-pig interspecies chimeras; and techniques for transplanting mouse neural stem cells into a mouse disease model for stroke. Part Three concludes the book with a look at ongoing ethical controversies and new scientific directions. Chapters in this part cover the ethics of crossing the xenobarrier; animal welfare; experimentation with spermatogonial stem cells; and cautious approaches to human-monkey chimera studies to further understand complex human brain disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Chimera Research: Methods and Protocols is a valuable resource for scientists interesting in using chimeras as a research tool while also taking into consideration their complex ethical scopes.
Genetic variability is an important parameter for plant breeders in any con ventional crop improvement programme. Very often the desired variation is un available in the right combination, or simply does not exist at all. However, plant breeders have successfully recombined the desired genes from cultivated crop gerrnplasm and related wild species by sexual hybridization, and have been able to develop new cultivars with desirable agronomie traits, such as high yield, disease, pest, and drought resistance. So far, conventional breeding methods have managed to feed the world's ever-growing population. Continued population growth, no further scope of expanding arable land, soil degradation, environ mental pollution and global warrning are causes of concern to plant biologists and planners. Plant breeders are under continuous pressure to improve and develop new cultivars for sustainable food production. However, it takes several years to develop a new cultivar. Therefore, they have to look for new technologies, which could be combined with conventional methods to create more genetic variability, and reduce the time in developing new cultivars, with early-maturity, and improved yield. The first report on induced mutation of a gene by HJ. Muller in 1927 was a major mi1estone in enhancing variation, and also indicated the potential applica tions of mutagenesis in plant improvement. Radiation sources, such as X-rays, gamma rays and fast neutrons, and chemical mutagens (e. g. , ethyl methane sulphonate) have been widely used to induce mutations.