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Abstract: Results of a cooperative survey of the central and eastern basins of Lake Erie in 1928-29 by the U.S. Bureau of Fisheries, New York State Conservation Department, Ontario Department of Game and Fisheries, Health Department of the City of Buffalo, and the Buffalo Society of Natural Sciences are presented in a series of papers. Physicochemical data include seasonal, vertical, and horizontal variations in temperatures, water movements, dissolved oxygen, carbon dioxide, phenolphthalein and methyl-orange alkalinity, pH, chlorides, and turbidity. The species composition, seasonal abundance, and distribution of micro- and macroplankton are discussed in detail. Special consideration is given to the influence of polluted river waters which flow into the lake. It is concluded that the lake is remarkably free from chemical and sewage pollution. Evidence of pollution farther than 1 mile from possible sources was detected at only 2 stations. The nutrient level of Lake Erie is high and the lake should support large fish populations.