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This book delves into the recent developments in the microscale and microfluidic technologies that allow manipulation at the single and cell aggregate level. Expert authors review the dominant mechanisms that manipulate and sort biological structures, making this a state-of-the-art overview of conventional cell sorting techniques, the principles of microfluidics, and of microfluidic devices. All chapters highlight the benefits and drawbacks of each technique they discuss, which include magnetic, electrical, optical, acoustic, gravity/sedimentation, inertial, deformability, and aqueous two-phase systems as the dominant mechanisms utilized by microfluidic devices to handle biological samples. Each chapter explains the physics of the mechanism at work, and reviews common geometries and devices to help readers decide the type of style of device required for various applications. This book is appropriate for graduate-level biomedical engineering and analytical chemistry students, as well as engineers and scientists working in the biotechnology industry.
Electrical Manipulation of Cells provides an authoritative and up-to-date review of the field, covering all the major techniques in a single source. The book features broad coverage that ranges from the mechanisms of action of external electrical fields on biological material to the ways in which electrical stimuli are employed to manipulate cells. Bringing together the work of leading international authorities, the book covers membrane breakdown, gene delivery, electroporation, electrostimulation, cell movement, hybridoma production, plant protoplasts, electrorotation and stimulation, and electromagnetic stimulation. For each topic, the authors discuss the relevance of the approach to the current state of the art of biotechnology. Electrical Manipulation of Cells is an unmatched source of information for anyone involved in the manipulation of cells, particularly biotechnologists, cell biology, microbiologists, biophysicists and plant scientists. For researchers, the book provides technical material that ccan be employed in their own work. Students will gain thorough appreciation of the applications of this important technique.
Electromanipulation of Cells is the first comprehensive, balanced overview of this dynamic discipline. Edited by leading authorities in the field, the book surveys state-of-the-art research as well as recent practical applications of electric field technologies.
This book is a printed edition of the Special Issue "Microdevices and Microsystems for Cell Manipulation" that was published in Micromachines
In this work, alternating current (AC) electric fields are used in combination with microfluidics to manipulate micro- and nano-sized particles and to probe the electrical characteristics of microchannels with potential application in portable diagnostics. This work was carried out as contribution to a collaborative research project involving researchers from chemistry, electrical engineering and mechanical engineering at the University of Victoria, in addition to researchers from the BC Cancer Deeley Research Centre. The manipulation of particles or cells within a microchannel flow is central to many microfluidic applications. In the context of diagnostics that utilize antibodies in serum, for example, the removal of cells from the sample is often required. Continuous removal of particles and cells is particularly critical in the case of flow-through nanohole array based sensing, as these serve as fine filters and thus are very susceptible to clogging. In this work, chevron shaped, interdigitated electrodes are used to produce dielectrophoretic forces in combination with hydrodynamic drag to displace particles from their corresponding streamlines to the center of a microchannel. Analytical and finite element modeling are used to provide insight into the focusing mechanism. Dielectrophoresis (DEP) also offers opportunities for particle manipulation in combination with porous media. In this preliminary work, the viability of dielectrophoresis tuned nano-particle transport in a nanohole array is investigated through analytical and numerical modeling. The effects of hydrodynamic drag and Brownian motion are considered in the context of applied voltage, flow rate and particle size. Preliminary flow-through tests are performed experimentally as proof of concept. The final contribution focuses primarily on external infrastructure that enables AC microfluidic diagnostics, with particular relevance to portable device applications and so-called point-of-care devices. Cell ph.
The past two decades have seen rapid development of micro-/nanotechnologies with the integration of chemical engineering, biomedical engineering, chemistry, and life sciences to form bio-MEMS or lab-on-chip devices that help us perform cellular analysis in a complex micro-/nanoflluidic environment with minimum sample consumption and have potential biomedical applications. To date, few books have been published in this field, and researchers are unable to find specialized content. This book compiles cutting-edge research on cell manipulation, separation, and analysis using microfluidics and bio-MEMS devices. It illustrates the use of micro-robots for biomedical applications, vascularized microfluidic organs-on-a-chip and their applications, as well as DNA gene microarray biochips and their applications. In addition, it elaborates on neuronal cell activity in microfluidic compartments, microvasculature and microarray gene patterning, different physical methods for drug delivery and analysis, micro-/nanoparticle preparation and separation in a micro-/nanofluidic environment, and the potential biomedical applications of micro-/nanoparticles. This book can be used by academic researchers, especially those involved in biomicrofluidics and bio-MEMS, and undergraduate- and graduate-level students of bio-MEMS/bio-nanoelectromechanical systems (bio-NEMS), biomicrofluidics, biomicrofabricatios, micro-/nanofluidics, biophysics, single-cell analysis, bionanotechnology, drug delivery systems, and biomedical micro-/nanodevices. Readers can gain knowledge of different aspects of microfluidics and bio-MEMS devices; their design, fabrication, and integration; and biomedical applications. The book will also help biotechnology-based industries, where research and development is ongoing in cell-based analysis, diagnosis, and drug screening.
This book is a printed edition of the Special Issue "Selected Papers from 2017 International Conference on Micro/Nanomachines" that was published in Micromachines
AC Electrokinetics is a very important phenomenon in the presence of non-uniform electric fields that is suited for direct manipulation of both particles and bulk fluid in the microfluidic system. Based on the parameters of the applied AC electric field such as voltage and frequency, as well as the properties of solution and the particles, for example, conductivity and permittivity, dominant forces in the microfluidic system may vary.