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This book explores the significance of soil microbial diversity to understand its utility in soil functions, ecosystem services, environmental sustainability, and achieving the sustainable development goals. With a focus on agriculture and environment, the book highlights the importance of the microbial world by providing state-of-the-art technologies for examining the structural and functional attributes of soil microbial diversity for applications in healthcare, industrial biotechnology, and bioremediation studies. In seven chapters, the book will act as a primer for students, environmental biotechnologists, microbial ecologists, plant scientists, and agricultural microbiologists. Chapter 1 introduces readers to the soil microbiome, and chapter 2 discusses the below ground microbial world. Chapter 3 addresses various methods for exploring microbial diversity, chapter 4 discusses the genomics methods, chapter 5 provides the metaproteomics and metatranscriptomics approaches and chapter 6 details the bioinformatics tools for soil microbial community analysis, and chapter 7 concludes the text with future perspectives on further soil microbial uses and applications.
Biochar Application: Essential Soil Microbial Ecology outlines the cutting-edge research on the interactions of complex microbial populations and their functional, structural, and compositional dynamics, as well as the microbial ecology of biochar application to soil, the use of different phyto-chemical analyses, possibilities for future research, and recommendations for climate change policy. Biochar, or charcoal produced from plant matter and applied to soil, has become increasingly recognized as having the potential to address multiple contemporary concerns, such as agricultural productivity and contaminated ecosystem amelioration, primarily by removing carbon dioxide from the atmosphere and improving soil functions. Biochar Application is the first reference to offer a complete assessment of the various impacts of biochar on soil and ecosystems, and includes chapters analyzing all aspects of biochar technology and application to soil, from ecogenomic analyses and application ratios to nutrient cycling and next generation sequencing. Written by a team of international authors with interdisciplinary knowledge of biochar, this reference will provide a platform where collaborating teams can find a common resource to establish outcomes and identify future research needs throughout the world. - Includes multiple tables and figures per chapter to aid in analysis and understanding - Includes a comprehensive table of the methods used within the contents, ecosystems, contaminants, future research, and application opportunities explored in the book - Includes knowledge gaps and directions of future research to stimulate further discussion in the field and in climate change policy - Outlines the latest research on the interactions of complex microbial populations and their functional, structural, and compositional dynamics - Offers an assessment of the impacts of biochar on soil and ecosystems
This volume provides methods, protocols, and reviews that are useful for new and experienced plant microbiome researchers. Chapters guide readers through the investigation of microbiomes associated with seeds, sampling microbiomes from plant compartments and tissues, culture-based methods, culture-independent metabarcoding methods, methods to obtain DNA and perform metabarcoding, protocols to block PCR amplification from the plant host, qPCR-based methods, editing of specific genes in Bacillus genomes, and Streptomycetes and plant microbial indicators. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Plant Microbiome: Methods and Protocols aims to ensure successful results in the further study of this vital field.
This book presents a detailed discussion on the direct interactions of plants and microorganisms in the rhizosphere environment. It includes fifteen chapters, each focusing on a specific component of plant-microbe interactions, such as the influence of plants on the root microbiome, and the downstream effects of rhizosphere microbial dynamics on carbon and nutrient fluxes in the surroundings. As such, the book helps readers gain a better understanding of diversity above the ground, and its effect on the microbiome and its functionality.
This book offers up-to-date information on different microbiomes, their community composition and interactive functions with the host, bringing together information from diverse research reports to provide an overview of the rapid developments in meta-omics technologies. It is a valuable resource for scientists, researchers, postgraduate and graduate students interested in understanding the impact and importance of next generation sequencing technologies on different hosts and their microbiomes.
Beginning with the germ theory of disease in the 19th century and extending through most of the 20th century, microbes were believed to live their lives as solitary, unicellular, disease-causing organisms . This perception stemmed from the focus of most investigators on organisms that could be grown in the laboratory as cellular monocultures, often dispersed in liquid, and under ambient conditions of temperature, lighting, and humidity. Most such inquiries were designed to identify microbial pathogens by satisfying Koch's postulates.3 This pathogen-centric approach to the study of microorganisms produced a metaphorical "war" against these microbial invaders waged with antibiotic therapies, while simultaneously obscuring the dynamic relationships that exist among and between host organisms and their associated microorganisms-only a tiny fraction of which act as pathogens. Despite their obvious importance, very little is actually known about the processes and factors that influence the assembly, function, and stability of microbial communities. Gaining this knowledge will require a seismic shift away from the study of individual microbes in isolation to inquiries into the nature of diverse and often complex microbial communities, the forces that shape them, and their relationships with other communities and organisms, including their multicellular hosts. On March 6 and 7, 2012, the Institute of Medicine's (IOM's) Forum on Microbial Threats hosted a public workshop to explore the emerging science of the "social biology" of microbial communities. Workshop presentations and discussions embraced a wide spectrum of topics, experimental systems, and theoretical perspectives representative of the current, multifaceted exploration of the microbial frontier. Participants discussed ecological, evolutionary, and genetic factors contributing to the assembly, function, and stability of microbial communities; how microbial communities adapt and respond to environmental stimuli; theoretical and experimental approaches to advance this nascent field; and potential applications of knowledge gained from the study of microbial communities for the improvement of human, animal, plant, and ecosystem health and toward a deeper understanding of microbial diversity and evolution. The Social Biology of Microbial Communities: Workshop Summary further explains the happenings of the workshop.
Unravelling Plant-Microbe Synergy focuses on agriculturally important microorganisms (AIM's) that are indigenous to soil and roots of the plant. These microbes contributing to nutrient balance, growth regulators, suppressing pathogens, alleviate stress response, orchestrating immune response and improving crop performance as they are offering sustainable and alternative solutions to the use of chemicals in agriculture. As plant microbe synergy is an enthralling subject, is multidisciplinary in nature, and concerns scientists involved in applied, and environmental microbiology and plant health and plant protection, Unravelling Plant-Microbe Synergy is an ideal resource that emphasizes the current trends of, and probable future of, microbes mediated amelioration of abiotic and biotic stress, agriculture sustainability, induced systemic tolerance and plant health protection. Unravelling Plant-Microbe Synergy discloses the microbial interaction for stress management and provides a better understanding to know the recent mechanisms to cope these environmental stresses. Unravelling Plant-Microbe Synergy bridges the gap in recent advances in the microbes interaction and rhizosphere engineering. - Emphasizes the plant microbes interactions, induced systemic tolerance, stress responsive genes and diversity of microorganisms - Illustrates the current impact of climate change on plant productivity along with mitigation strategies - Provides a two-way interactive approach to both plants and microbes, and includes multi-omics approaches
Below the soil surface, the rhizosphere is the dynamic interface among plant roots, soil microbes and fauna, and the soil itself, where biological as well as physico-chemical properties differ radically from those of bulk soil. The Rhizosphere is the first ecologically-focused book that explicitly establishes the links from extraordinarily small-scale processes in the rhizosphere to larger-scale belowground patterns and processes. This book includes chapters that emphasize the effects of rhizosphere biology on long-term soil development, agro-ecosystem management and responses of ecosystems to global change. Overall, the volume seeks to spur development of cross-scale links for understanding belowground function in varied natural and managed ecosystems. - First cross-scale ecologically-focused integration of information at the frontier of root, microbial, and soil faunal biology - Establishes the links from extraordinarily small-scale processes in the rhizosphere to larger-scale belowground patterns and processes - Includes valuable information on ecosystem response to increased atmospheric carbon dioxide and enhanced global nitrogen deposition - Chapters written by a variety of experts, including soil scientists, microbial and soil faunal ecologists, and plant biologists
This two-volume work is a testament to the increasing interest in the role of microbes in sustainable agriculture and food security. Advances in microbial technologies are explored in chapters dealing with topics such as carbon sequestration, soil fertility management, sustainable crop production, and microbial signaling networks. Volume I is a collection of research findings that invites readers to examine the application of microbes in reinstating degraded ecosystems and also in establishing sustainable croplands. Highly readable entries attempt to close the knowledge gap between soil microbial associations and sustainable agriculture. An increase in the global population with changing climate is leading to environments of various abiotic and biotic stresses for agricultural crops. It therefore becomes important to identify the techniques to improve soil fertility and function using different microbial groups such as actinobacteria, microalgae, fluorescent pseudomonads and cyanobacterial systems. These are examined in this volume in greater detail. This work is a significant contribution to research in this increasingly important discipline, and will appeal to researchers in microbiology, agriculture, environmental sciences, and soil and crop sciences.
This book includes 25 contributions from vastly experienced, global experts in PGPR research in a comprehensive and influential manner, with the most recent facts and extended case studies. Also, the chapters address the current global issues in biopesticide research.