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In this book international experts discuss the state-of-the-art in the biological degradation of hydrocarbons to meet remedial or disposal goals. The work focuses on practical applications, often on globally important scales including the remediation of some of the world’s largest crude oil spills. Other related chapters discuss important implications of microbial transformation of hydrocarbons, including treatment of high fat processing wastes, impacts of microbial biodegradation activity on industrial processes, and the implications of microbial oil degradation in relation to modern oil extraction processes like hydraulic fracturing of shales and extraction of oil sands.
This Volume describes methods for simulating natural environments by using reproducible and controllable meso- and microcosm experiments to analyse hydrocarbon-degrading microorganisms and to test hypotheses. It presents important aspects of the preparation of experimental set-ups, hydrocarbon application and sampling, and features protocols for experiments with different types of samples, such as biofilms, aquatic systems (e.g. groundwater, streams), and sediments, including oscillating oxic-anoxic mesocosms, as well as for in-situ experimentation in subtidal and deep sediments. Two chapters are dedicated to cultivation under high-pressure conditions, and several chapters include protocols for processing samples for downstream chemical, microbial or activity analyses. Several of the approaches presented are generic and will benefit anyone embarking on designing meso- and microcosm experiments. pbHydrocarbon and Lipid Microbiology Protocols/b There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
The presence of chemicals in our environment is a subject of intense interest owing to the many potential adverse health effects to humans following exposure to these chemicals. The principles and practices of risk assessment are used to assess the associated health risks to provide a scientific and health basis for guidance or regulatory standards
A textbook and resource for professional scientists working in the areas of industrial microbiology, environmental microbiology, and biodegradation. - Details both the fundamental concepts of the microbial transformation of organic compounds as well as its application for biotechnology and biodegradation. - Offers comprehensive coverage of microbial catabolism from the group that developed the online Biocatalysis/Biodegradation Database (http: //umbbd.msi.umn.edu/). - Discusses the logic of catabolism, which is important in the context of genome annotation and predicting biodegradation reactions.
Contamination of the environment is of enormous economic and environmental significance. This book reviews the latest research on the harnessing of biotechnology to decontaminate the environment. Internationally acclaimed authors from diverse fields present comprehensive reviews of the most important and well-established solutions to environmental contamination. The limitations as well as the future potential of these practices are critically highlighted. The book also includes stimulating reviews of selected practices which are either novel or the subject of some debate in bioremediation research and practice. This is essential reading for bioremediation researchers and practitioners who wish to look beyond current practice and explore new avenues that may ultimately change the way in which bioremediation is conducted, monitored and studied.
This book provides an account of the major environmental contaminations present today, and offers detailed insights into their potential remediation through bio-based solutions. Bringing together the work of various international experts in this field, it contains comprehensive reviews on the mechanisms of bioremediation. Moreover, the book discusses the strategies by which bacteria and plants help in the decontamination of environmental pollutants. As such, it represents a valuable resource for a wide audience, including environmental scientists, biochemists, soil scientists, botanists, agronomists and molecular biologists.