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Advanced 3D-Printed Systems and Nanosystems for Drug Delivery and Tissue Engineering explores the intricacies of nanostructures and 3D printed systems in terms of their design as drug delivery or tissue engineering devices, their further evaluations and diverse applications. The book highlights the most recent advances in both nanosystems and 3D-printed systems for both drug delivery and tissue engineering applications. It discusses the convergence of biofabrication with nanotechnology, constructing a directional customizable biomaterial arrangement for promoting tissue regeneration, combined with the potential for controlled bioactive delivery. These discussions provide a new viewpoint for both biomaterials scientists and pharmaceutical scientists. Shows how nanotechnology and 3D printing are being used to create systems which are intelligent, biomimetic and customizable to the patient Explores the current generation of nanostructured 3D printed medical devices Assesses the major challenges of using 3D printed nanosystems for the manufacture of new pharmaceuticals
This book introduces readers to the theory and practice of extrusion bio-printing of scaffolds for tissue engineering applications. The author emphasizes the fundamentals and practical applications of extrusion bio-printing to scaffold fabrication, in a manner particularly suitable for those who wish to master the subject matter and apply it to real tissue engineering applications. Readers will learn to design, fabricate, and characterize tissue scaffolds to be created by means of extrusion bio-printing technology.
Three-dimensional (3D) printing enables the fabrication of tissue-engineered constructs and devices from a patient’s own medical data, leading to the creation of anatomically matched and patient-specific constructs. There is a growing interest in applying 3D printing technologies in the fields of tissue engineering and regenerative medicine. The main printing methods include extrusion-based, vat photopolymerization, droplet-based, and powder-based printing. A variety of materials have been used for printing, from metal alloys and ceramics to polymers and elastomers as well as from hydrogels to extracellular matrix proteins. More recently, bioprinting, a subcategory of 3D printing, has enabled the precise assembly of cell-laden biomaterials (i.e., bioinks) for the construction of complex 3D functional living tissues or artificial organs. In this Special Issue, we aim to capture state-of-the-art research papers and the most current review papers focusing on 3D printing for tissue engineering and regenerative medicine. In particular, we seek novel studies on the development of 3D printing and bioprinting approaches, developing printable materials (inks and bioinks), and utilizing 3D-printed scaffolds for tissue engineering and regenerative medicine applications. These applications are not limited to but include scaffolds for in vivo tissue regeneration and tissue analogues for in vitro disease modeling and/or drug screening.
Tissue Printing explains and compiles step-by-step methods and applications of this elegantly simple and practical technique. The protocols can be easily modified by the research biologist or teacher to study a wide variety of biological problems for basic research or classroom teaching. Tissue printing requires no expensive equipment for successful implementation, is safe, and can be used for both plant and animal systems. This practical laboratory guide contains many illustrative halftones, a complete bibliography, technique overviews, detailed protocols, and sample practical applications. * Provides step-by-step protocols and practical applications of tissue printing to the plant and animal sciences* Describes simple, rapid, low-budget techniques for study of proteins and nucleic acids at the tissue level* Protocols have been tested and successfully used by authors of each chapter and their colleagues* Chapters are thoroughly illustrated and extensively referenced to original literature.
Cell and organ printing has become a hot topic of scientific pursuit. Since several early publications between 2000-2003 that demonstrated proof-of-concept, cell and organ printing has blossomed into a rich area for scientific exploration that is being performed by researchers across the globe. Research has thoroughly demonstrated that living cells can be printed via a number of actuations including electrospray, extrusion via micropens and ejection through photothermal, thermal or optical mechanisms. This topic has come of age and it is ripe for exploring the underpinnings of the research to date. We have included research that uses printing technology to deposit or guide cells for tissue engineering applications and for completeness, we have also included chapters describing bacteria printing, biomolecular printing that could be used to build growth factors or recruitment macromolecules into scaffolds, tissue microdissection, as well as live cell printing. The breadth of approaches includes 3D freeform fabrication, ink jet, laser guidance and modified laser direct write techniques. We hope that this book is not the final word but the first word, defining how these tools have been used to take the first steps towards the ultimate goal of creating heterogeneous tissue constructs. Only time will tell whether cell printers will truly become organ printers, but the technologies described in this book hold promise to achieve what the field of regenerative medicine requires - functional 3D scaffolds with multiple cell types differentiated into functional tissue!
Essentials of 3D Biofabrication and Translation discusses the techniques that are making bioprinting a viable alternative in regenerative medicine. The book runs the gamut of topics related to the subject, including hydrogels and polymers, nanotechnology, toxicity testing, and drug screening platforms, also introducing current applications in the cardiac, skeletal, and nervous systems, and organ construction. Leaders in clinical medicine and translational science provide a global perspective of the transformative nature of this field, including the use of cells, biomaterials, and macromolecules to create basic building blocks of tissues and organs, all of which are driving the field of biofabrication to transform regenerative medicine. Provides a new and versatile method to fabricating living tissue Discusses future applications for 3D bioprinting technologies, including use in the cardiac, skeletal, and nervous systems, and organ construction Describes current approaches and future challenges for translational science Runs the gamut of topics related to the subject, from hydrogels and polymers to nanotechnology, toxicity testing, and drug screening platforms
This book introduces various 3D printing systems, biomaterials, and cells for organ printing. In view of the latest applications of several 3D printing systems, their advantages and disadvantages are also discussed. A basic understanding of the entire spectrum of organ printing provides pragmatic insight into the mechanisms, methods, and applications of this discipline. Organ printing is being applied in the tissue engineering field with the purpose of developing tissue/organ constructs for the regeneration of both hard (bone, cartilage, osteochondral) and soft tissues (heart). There are other potential application areas including tissue/organ models, disease/cancer models, and models for physiology and pathology, where in vitro 3D multicellular structures developed by organ printing are valuable.
This text advances fundamental knowledge in modeling in vitro tissues/organs as an alternative to 2D cell culture and animal testing. Prior to engineering in vitro tissues/organs,the descriptions of prerequisites (from pre-processing to post-processing) in modeling in vitro tissues/organs are discussed. The most prevalent technologies that have been widely used for establishing the in vitro tissue/organ models are also described, including transwell, cell spheroids/sheets, organoids, and microfluidic-based chips. In particular, the authors focus on 3D bioprinting in vitro tissue/organ models using tissue-specific bioinks. Several representative bioprinting methods and conventional bioinks are introduced. As a bioink source, decellularized extracellular matrix (dECM) are importantly covered, including decellularization methods, evaluation methods for demonstrating successful decellularization, and material safety. Taken together, the authors delineate various application examples of 3D bioprinted in vitro tissue/organ models especially using dECM bioinks.
This book offers the first comprehensive review of emerging cell-printing technologies, from the use of printing technology to deposit or guide cells for tissue engineering applications, to bacteria printing, biomolecular printing and live cell printing.
Carbon Transfer Printing is a book about one of the earliest photographic processes that provided the first permanent printing methods, available in one form or another for over 150 years. This book reviews the extensive history of carbon transfer and related pigment processes in both monochrome and color, to serve as point source for a new carbon printer to begin to master the craft of carbon printing, as well as provide new material for experienced carbon printers so they can expand their techniques. The book includes never-before-published information on pre-sensitizing carbon tissue with newly identified compounds, information on the safe use and disposal of hexavalent chromium compounds, and simplified methods of producing 3-color carbon prints. Carbon Transfer Printing is divided into two parts, illustrated with 175 photographs. Part One is a complete how-to on the carbon transfer process, from simple to complex, with a troubleshooting guide and an extensive chapter on digital negatives. Part Two is devoted to contemporary carbon printers who share their methods and secrets to creating their beautiful carbon prints. Topics that the book covers are: Key events in carbon’s history How to organize the carbon workplace Sections on necessary supplies and equipment A step-by-step digital method of making high quality digital negatives Simple and advanced methods of carbon printing How to make carbon tissue, including several methods of pre-sensitizing How to choose UV light sources for printing in carbon Step-by-step processing directions How to prepare final support papers Troubleshooting carbon Multi-layer printing to add tone, or make a full color carbon print Finishing and final presentation of carbon prints A gallery of images and advice from contemporary carbon printers Carbon Transfer Printing is designed for both the beginning carbon enthusiast as well as for the advanced practitioner. Backed with extensive research on carbon printing from books, journals, and magazine articles from the 1800s to present day, and the extensive personal experience in carbon printing of the authors, there is enough information in this book to provide inspiration and proof of both the glorious past of carbon printmaking and its enduring importance to a new generation of image makers who value the handmade print.