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Brings together key new results of interdisciplinary collaborations among various research fields on rhodopsin including the photoreceptive mechanism of rhodopsins, the molecular mechanism of the visual transduction process, visual processes in the retina and other transduction processes in the retina and brain. The structures of the rhodopsin molecule are studied in the fields of protein chemistry, molecular biology, organic chemistry and structural biology; the ultra fast reactions of the retinal protein are studied in physics, biophysics, physical chemistry, organic chemistry and photobiology; the phototransduction in retinal proteins and visual cells are studied in biophysics, biochemistry, biophysical chemistry and photobiology; and the localization in the tissues is studied in anatomy and histochemistry. The diversity of visual systems in various animals is studied in zoology and comparative biochemistry.
This volume contains the lectures given at the NATO Advanced Study Institute on "Biophysics of Photoreceptors and Photomovements in Microorganisms" held in Tir renia (Pisa), Italy, in September 1990. The Institute was sponsored and mainly funded by the Scientific Affairs Division of NATO; the Physical Science Committee and the Institute of Biophysics of National Research Council of Italy also supported the School and substantially contributed to its success. It is our pleasant duty to thank these institu tions. Scientists from very different backgrounds contributed to the understanding of this fast developing field of research, which has seen considerable progress during the last years. The areas of expertise ranged from behavioral sciences, supported by sophi sticated techniques such as image analysis or laser light scattering, to spectroscopy, ap plied, in different time domains, to the study of the primary photoreactions, to electro physiology, biochemistry or molecular biology, with the aim of analyzing the various steps of the transduction chains and how they control the motor apparatus of the cells. The organisms studied covered a wide range, from bacteria to algae, fungi and other eukaryotes. Thus, the ASI represented a successful opportunity for carrying on and imple menting an interdisciplinary approach to the study of the biophysical basis of photore ception and photosensory transduction in aneural organisms, with special attention to the basic phenomena and the underlying molecular events. We hope that this book has caught the spirit in which the ASI was conceived.
Biological Events Probed by Ultrafast Laser Spectroscopy summarizes the progress in the experimental and theoretical understanding of primary phenomena occurring in biology on a picoseconds and nanosecond time scale. This book includes basic principles, survey of research results, and thinking of experts in the fields of photosynthesis, vision, hemoglobin, and DNA. This reference is organized into five parts. The primary events in the various areas of biology are reviewed in the first four parts. The last part covers the picosecond and subpicosecond laser techniques. Primary processes in photosynthesis are then discussed. This is followed by chapters on fluorescence and absorption kinetic measurements in higher plants. Subsequent chapters cover kinetic measurements in bacteria photosynthesis, theories of primary energy transfer appropriate to photosynthesis, and multiexcitation processes. Part II examines primary visual processes, including kinetic experimental work on rhodopsin and bacteriorhodopsin, and theoretical concepts on primary events in vision. The third part presents the introductory materials on the kinetic models of hemoglobin and myoglobin. It also discusses the interesting picosecond measurements on these proteins. Part IV focuses on the application of modern ultrafast temporal techniques to the study of DNA and its components, as well as DNA-dye complexes. The concluding part introduces the principles of design and operation of the solid state laser and picoseconds spectroscopy methods. A discussion on pulse generation methods and measurement techniques applicable for measuring primary events in biological systems is provided. This book is useful both for the newbies and experts in the field of ultrafast phenomena. It aims to attract biologists, chemists, physicists, and engineers who are interested in biological processes. It will help readers find all the necessary and relevant material in one presentation.
Semiannual, with semiannual and annual indexes. References to all scientific and technical literature coming from DOE, its laboratories, energy centers, and contractors. Includes all works deriving from DOE, other related government-sponsored information, and foreign nonnuclear information. Arranged under 39 categories, e.g., Biomedical sciences, basic studies; Biomedical sciences, applied studies; Health and safety; and Fusion energy. Entry gives bibliographical information and abstract. Corporate, author, subject, report number indexes.