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Chromatography and all the related separation techniques are experimental in their origin and justification. However, the spectacular progress made in this area since World War II has given rise to a theoretical underpinning. The present book covers the current status of the research area and places it in perspective with the general concepts of the fields of physical chemistry involved. The ASI lectures/authors -- well known leaders in their fields -- have written presentations at the graduate level, accessible to all those who have a good general background in the thermodynamics and mass transfer theory of phase equilibria. The book will be useful to young scientists and engineers who wish to access the current frontiers in chromatography and other separation sciences.
Handbook of Advanced Chromatography /Mass Spectrometry Techniques is a compendium of new and advanced analytical techniques that have been developed in recent years for analysis of all types of molecules in a variety of complex matrices, from foods to fuel to pharmaceuticals and more. Focusing on areas that are becoming widely used or growing rapidly, this is a comprehensive volume that describes both theoretical and practical aspects of advanced methods for analysis. Written by authors who have published the foundational works in the field, the chapters have an emphasis on lipids, but reach a broader audience by including advanced analytical techniques applied to a variety of fields. Handbook of Advanced Chromatography / Mass Spectrometry Techniques is the ideal reference for those just entering the analytical fields covered, but also for those experienced analysts who want a combination of an overview of the techniques plus specific and pragmatic details not often covered in journal reports. The authors provide, in one source, a synthesis of knowledge that is scattered across a multitude of literature articles. The combination of pragmatic hints and tips with theoretical concepts and demonstrated applications provides both breadth and depth to produce a valuable and enduring reference manual. It is well suited for advanced analytical instrumentation students as well as for analysts seeking additional knowledge or a deeper understanding of familiar techniques. - Includes UHPLC, HILIC, nano-liquid chromatographic separations, two-dimensional LC-MS (LCxLC), multiple parallel MS, 2D-GC (GCxGC) methodologies for lipids analysis, and more - Contains both practical and theoretical knowledge, providing core understanding for implementing modern chromatographic and mass spectrometric techniques - Presents chapters on the most popular and fastest-growing new techniques being implemented in diverse areas of research
Featuring reliable, up-to-the-minute reviews of major developments in chromatography, Volume 43 studies the latest advances in the field with contributions and current research from world-renowned leaders in industry. It provides detailed discussions of current topics, issues, and developments in separation science and examining topics such as grad
The basic objectives of this book are to: provide basic information on chromatography and separation science; show how simple extraction and partition processes provide the basis for development of chromatography and separation science; describe the role of chromatography and separation science in various fields; discuss the role of chromatography and separation science in development of new methodology; and present new evolving methods and how to select an optimum method.· The book covers the fundamental physical and chemical phenomena involved in separations· Provides a concise overview of the basics of transport phenomena and thermodynamics· Shows the importance of chromatography within separation science
An in-depth text that explores the interface between analytical chemistry and trace evidence Analytical Techniques in Forensic Science is a comprehensive guide written in accessible terms that examines the interface between analytical chemistry and trace evidence in forensic science. With contributions from noted experts on the topic, the text features a detailed introduction analysis in forensic science and then subsequent chapters explore the laboratory techniques grouped by shared operating principles. For each technique, the authors incorporate specific theory, application to forensic analytics, interpretation, forensic specific developments, and illustrative case studies. Forensic techniques covered include UV-Vis and vibrational spectroscopy, mass spectrometry and gas and liquid chromatography. The applications reviewed include evidence types such as fibers, paint, drugs and explosives. The authors highlight data collection, subsequent analysis, what information has been obtained and what this means in the context of a case. The text shows how analytical chemistry and trace evidence can problem solve the nature of much of forensic analysis. This important text: Puts the focus on trace evidence and analytical science Contains case studies that illustrate theory in practice Includes contributions from experts on the topics of instrumentation, theory, and case examples Explores novel and future applications for analytical techniques Written for undergraduate and graduate students in forensic chemistry and forensic practitioners and researchers, Analytical Techniques in Forensic Science offers a text that bridges the gap between introductory textbooks and professional level literature.
This book gives an overview of the numerical data analysis and signal treatment techniques that are used in chromatography and related separation techniques. Emphasis is given to the description of the symmetrical and asymmetrical chromatographic peak shape models. Both theoretical and empirical models are discussed.The fundamentals of data acquisition, types and effect of baseline noise, and methods of improving the signal-to-noise ratio (either in time or in frequency and wavelet domain) are thoroughly discussed. Resolution enhancement techniques, such as curve fitting, deconvolution by Fourier and wavelet transforms, iterative deconvolution, Kalman filtering and multivariate methods of curve resolution are all discussed with several chromatographic examples. Quantitative analysis by peak area of peak height measurement, the precision and accuracy of the quantitation of stand-alone or overlapping and symmetrical or asymmetrical peaks are treated. In a separate chapter, guidelines are given for the use of transform techniques for the analysis of chromatograms. A statistical description of peak overlap is given in the final chapters. Since the concept of resolution has to be reconsidered when one separates complex mixtures, the problem of resolution and overlap is quantitatively discussed by means of statistical methods, and by using Fourier analysis of the complex chromatogram.Features of this book• The ultimate source of numerical techniques to enhance chromatographic data• Gives a detailed description of signal and resolution enhancement techniques in a manner applicable for enhancing not only chromatography, but also spectroscopic and other analytical signals• The first book with a thorough overview of the statistics of peak overlap.This is the first volume to encompass both the simple and more sophisticated methods for the numerical treatment of chromatograms. It is, therefore, the fundamental resource of numerical analysis methods for every analyst.
Carbohydrate Analysis by Modern Liquid Phase Separation Techniques, Second Edition, presents readers with the various principles of modern liquid phase separation techniques and their contributions to the analysis of complex carbohydrates and glycoconjugates. In a selection of all-new chapters, this fully updated volume covers each technique in detail. The book aims to help analysts solve any of the many practical problems they may face in tackling the analysis of carbohydrates. In addition, it addresses current difficulties that must be resolved in carbohydrate research, thus inspiring further important technological developments to meet these challenges. This is an essential resource for anyone seeking a broad view of the science of carbohydrates and separation techniques. - Covers the basic principles of modern liquid phase separation techniques, along with their applications - Compiles up-to-date information on the field of carbohydrate analysis, along with updates on separation science - Focuses on problems currently faced in carbohydrate analysis and the solutions necessary for further progress
Separation science plays a critical role in maintaining our standard of living and quality of life. Many industrial processes and general necessities such as chemicals, medicines, clean water, safe food, and energy sources rely on chemical separations. However, the process of chemical separations is often overlooked during product development and this has led to inefficiency, unnecessary waste, and lack of consensus among chemists and engineers. A reevaluation of system design, establishment of standards, and an increased focus on the advancement of separation science are imperative in supporting increased efficiency, continued U.S. manufacturing competitiveness, and public welfare. A Research Agenda for Transforming Separation Science explores developments in the industry since the 1987 National Academies report, Separation and Purification: Critical Needs and Opportunities. Many needs stated in the original report remain today, in addition to a variety of new challenges due to improved detection limits, advances in medicine, and a recent emphasis on sustainability and environmental stewardship. This report examines emerging chemical separation technologies, relevant developments in intersecting disciplines, and gaps in existing research, and provides recommendations for the application of improved separation science technologies and processes. This research serves as a foundation for transforming separation science, which could reduce global energy use, improve human and environmental health, and advance more efficient practices in various industries.
Chromatography - A Century of Discovery 1900-2000 represents the combined thinking and contributions of many chromatographers. It includes several in-depth feature chapters covering the Beginnings of Chromatography, which highlights M.S. Tswett, the inventor of chromatography, and several other early pioneers. Included are the contributions of several Nobel Laureates,and 125 Chromatography Award Winners and contributors, an extensive bibliography of publications on the History of the Evolution of Chromatography; a presentation of Major International Symposia supporting chromatography and as a bridge to selected sciences. Special chapters are written by well-known Chromatographers on Support and Stationary Phases, and Separations followed by a chapter on Milestones and Paradigm Shifts in Science. New discoveries in the life sciences and medicine, agriculture, the environment and separations technology in the 21st century will rely immeasurably on the 20th century research tools in chromatography and those yet to be developed.
The last two decades have seen a number of significant advances in the methodology for evaluating the molecular weight distributions of polydispersed macromolecular systems in solution at the molecular level. This reference presents reviews on the progress in different analytical and characterization methods of biopolymers. Readers will find useful information about combinations of complex biopolymer analysis such as chromatographic or membrane based fractionation procedures combined with multiple detectors on line (multi-angle laser light scattering or MALLS). Key topics include: • refractive index, UV-Vis absorbance and intrinsic viscosity detection systems, • advances in SEC-MALLS (size exclusion chromatography coupled to multi-angle laser light scattering) and FFF-MALLS (field flow fractionation coupled on line to MALLS), • HPSEC-A4F-MALLS, matrix-assisted laser-desorption ionization (MALDI) • electrospray ionization (ESI) mass spectrometry • nuclear magnetic resonance (NMR) spectroscopy This reference is intended for students of applied chemistry and biochemistry who require information about biopolymer analysis and characterization.