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An introduction to the microscope with colored illustrations, projects, and activities.
This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material
Greg makes fascinating discoveries about things he finds at home when he looks at them through his new microscope. ‘An accurate and entertaining book for beginning independent readers.' 'BL.
Providing an overview of God's world through a microscope, this book gives a brief history of microscopes before diving into seeing the world through one. Starting with their simple origins in the 13th century as magnifying glasses and exploring some of the many modern varieties of imaging, we explore how they are used and some of what may be seen through one now.Filled with full-color microscopic images of varied animals, insects, plants and fungi, and microorganisms, as well as detailed information for using the modern microscope in the classroom.Discusses examples of stained and unstained slide samples, brightfield, darkfield, and phase contrast microscopy.Includes practical tips about the use of the microscope and labels many of the slide images for easier identification of microscopic structures.Though this is an independent text that can be used with any biology study, it also serves as a companion book in the Master's Class Biology: The Study of Life From a Christian Worldview high school course available from Master Books®. Those who purchase this book would not have to purchase a microscope in order to fulfill the requirements.
Excerpt from The Microscope, Vol. 12: An Illustrated Monthly Magazine for the Student of Natures Little Things In Offering this preliminary notice of my studies on the growth and decay Of plants, as seen in the onion, I feel that much time is needed to get its complete history free from all error - much more time than I now have at command. I hope that many others, both present workers and new recruits, may help to carry the work Onward. About the Publisher Forgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.com This book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully; any imperfections that remain are intentionally left to preserve the state of such historical works.
This book has evolved by processes of selection and expansion from its predecessor, Practical Scanning Electron Microscopy (PSEM), published by Plenum Press in 1975. The interaction of the authors with students at the Short Course on Scanning Electron Microscopy and X-Ray Microanalysis held annually at Lehigh University has helped greatly in developing this textbook. The material has been chosen to provide a student with a general introduction to the techniques of scanning electron microscopy and x-ray microanalysis suitable for application in such fields as biology, geology, solid state physics, and materials science. Following the format of PSEM, this book gives the student a basic knowledge of (1) the user-controlled functions of the electron optics of the scanning electron microscope and electron microprobe, (2) the characteristics of electron-beam-sample inter actions, (3) image formation and interpretation, (4) x-ray spectrometry, and (5) quantitative x-ray microanalysis. Each of these topics has been updated and in most cases expanded over the material presented in PSEM in order to give the reader sufficient coverage to understand these topics and apply the information in the laboratory. Throughout the text, we have attempted to emphasize practical aspects of the techniques, describing those instru ment parameters which the microscopist can and must manipulate to obtain optimum information from the specimen. Certain areas in particular have been expanded in response to their increasing importance in the SEM field. Thus energy-dispersive x-ray spectrometry, which has undergone a tremendous surge in growth, is treated in substantial detail.
An in-depth guide explains how to put bugs, water, food, plants and pollen, and even parts of the body (like fingernails) under the scope for a close-up glimpse while also explaining how to identify the microscope's different pieces and how to focus properly.
This is a brief history of the development of microscopy, from the use of beads and water droplets in ancient Greece, through the simple magnifying glass, to the modern compound microscope. The technology and optical theory are developed in a straightforward manner, and this leads to a description and explanation of the most modern technologies in electron microscopy, and scanning electron microscopy as well as the new scanning probe microscopies. A series of very interesting applications of the various microscopic techniques are described. The most recent pioneering techniques in near field and confocal optical microscope technologies are described and evaluated for their future importance.
This manual contains selected material from Cells - a Laboratory Manual, as well as two chapters from Live Cell Imaging. It includes sections on microscopy, and on preparing and labelling specimens for microscopy.