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Excerpt from The Microscope, Vol. 12: An Illustrated Monthly Magazine for the Student of Natures Little Things In Offering this preliminary notice of my studies on the growth and decay Of plants, as seen in the onion, I feel that much time is needed to get its complete history free from all error - much more time than I now have at command. I hope that many others, both present workers and new recruits, may help to carry the work Onward. About the Publisher Forgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.com This book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully; any imperfections that remain are intentionally left to preserve the state of such historical works.
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Excerpt from The Microscope, 1892, Vol. 12: An Illustrated Monthly Magazine for the Student of Nature's Little Things Microooocus oromrsns (raovxsronu. Name, perhaps), N. S. Oocci, very large, oval, oblong, or less frequently spherical, with a bright spore or nucleus variously placed; light translucent bodies with a very thin cell wall and clearish contents. Measure ments of those of circular outlines 4, rarely 5, u, in diameter; of the ovals 4, 5, 6, 7, 8, in longest diameter, rare individuals much longer, by 3, 4, 511 wide, or slightly more or less; the very longest specimens often contain two or three nuclei, but no chain-like forms; sometimes none of these would be seen in a dense field of the cocci. This is decidedly the largest, most bold-looking Micrococcus I have yet seen or have read of. It is magnificent. At first I thought I had large fields full of some mould spores. It occurs singly, often so numerous in old rot ting onion bulbs as to fill the field of the microscope very densely in a single layer. It appears soon after the Bacterium refringente, also in the water-mounted slides kept for several days. About the Publisher Forgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.com This book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully; any imperfections that remain are intentionally left to preserve the state of such historical works.
This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material
THE GRIFFITH PROJECT Paolo Cherchi Usai, General Editor Volume 12: Essays on D.W. Griffith Edited by Paolo Cherchi Usai and Cynthia Rowell With contributions by William M. Drew, Helmut Färber, André Gaudreault, Philippe Gauthier, Lea Jacobs, Joyce Jesionowski, Charlie Keil, Richard Koszarski, Arthur Lennig, Pat Loughney, David Mayer, Russell Merritt, Jan Olsson, Paul Spehr, Yuri Tsivian, Linda Williams In early 1996, an international group of 35 specialists in silent cinema volunteered to write commentaries on more than six hundred films directed, written, produced and supervised by D.W. Griffith – or featuring him as a performer – for the eleven-volume series The Griffith Project, the largest monograph ever assembled on an individual film director, in conjunction with the massive retrospective held at the Pordenone Silent Film Festival from 1996 to 2008. All authors involved in The Griffith Project were bound to strict editorial rules, most notably the fact that all titles in the series would be assigned to them in pre-determined groups rather than as a result of their own individual preference for this or that specific entry. The patience and commitment demonstrated by all scholars in this endeavor requires at least a symbolic recognition. We therefore invited the members of the project team to write an essay on a (D.W. Griffith-related) topic of their own choice. The papers included in this volume constitute the response to our carte blanche invitation. Our offer was also extended to other experts on D.W. Griffith who, for various reasons, were unable to participate in The Griffith Project but consistently supported it with their generous advice and insight. This volume brings The Griffith Project to completion, as 2008 sees the last installment of the D.W. Griffith program at the Pordenone Silent Film Festival with the screening of his films produced between 1925 and 1931. Not surprisingly, twelve years of research on D.W. Griffith have unearthed an impressive wealth of knowledge but also an equally amazing array of new questions, certainly enough of them to fill several more volumes. Some of them (including the increasingly complex issue of D.W. Griffith's role as production supervisor) are only introduced or barely mentioned here, but we are confident that what we have called the 'Griffith Project' will continue – at the Giornate and elsewhere – with more research and newly found or preserved prints. PAOLO CHERCHI USAI is Director of the National Film and Sound Archive of Australia. He is co-founder of the Pordenone Silent Film Festival and of the L. Jeffrey Selznick School of Film Preservation at George Eastman House (Rochester, New York). He directed the experimental feature film Passio (2007). His latest book is David Wark Griffith (Editrice Il Castoro, 2008).
This book has evolved by processes of selection and expansion from its predecessor, Practical Scanning Electron Microscopy (PSEM), published by Plenum Press in 1975. The interaction of the authors with students at the Short Course on Scanning Electron Microscopy and X-Ray Microanalysis held annually at Lehigh University has helped greatly in developing this textbook. The material has been chosen to provide a student with a general introduction to the techniques of scanning electron microscopy and x-ray microanalysis suitable for application in such fields as biology, geology, solid state physics, and materials science. Following the format of PSEM, this book gives the student a basic knowledge of (1) the user-controlled functions of the electron optics of the scanning electron microscope and electron microprobe, (2) the characteristics of electron-beam-sample inter actions, (3) image formation and interpretation, (4) x-ray spectrometry, and (5) quantitative x-ray microanalysis. Each of these topics has been updated and in most cases expanded over the material presented in PSEM in order to give the reader sufficient coverage to understand these topics and apply the information in the laboratory. Throughout the text, we have attempted to emphasize practical aspects of the techniques, describing those instru ment parameters which the microscopist can and must manipulate to obtain optimum information from the specimen. Certain areas in particular have been expanded in response to their increasing importance in the SEM field. Thus energy-dispersive x-ray spectrometry, which has undergone a tremendous surge in growth, is treated in substantial detail.
Excerpt from The Microscope, Vol. 1: An Illustrated Monthly Designed to Popularize the Subject of Microscopy, New Series (Nos; 1-12) Dust or Cloudiness on the Ocular. - Employ the 18 mm. (2 in.) objective low ocular, and fiy's wing as object. About the Publisher Forgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.com This book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully; any imperfections that remain are intentionally left to preserve the state of such historical works.