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Induced mutagenesis is a common and promising method for screening for new crops with improved properties. This title introduces the different methods and then focuses on the screening, detection and analysis of the novel mutations. Written by a global team of authors the book is an indispensable tool for all scientists working on crop breeding in industry and academia.
Induced mutagenesis is a common and promising method for screening for new crops with improved properties. This title introduces the different methods and then focuses on the screening, detection and analysis of the novel mutations. Written by a global team of authors the book is an indispensable tool for all scientists working on crop breeding in industry and academia.
Abstract: This book presents contemporary information on mutagenesis in plants and its applications in plant breeding and research. The topics are classified into sections focusing on the concepts, historical development and genetic basis of plant mutation breeding (chapters 1-6); mutagens and induced mutagenesis (chapters 7-13); mutation induction and mutant development (chapters 14-23); mutation breeding (chapters 24-34); or mutations in functional genomics (chapters 35-41). This book is an essential reference for those who are conducting research on mutagenesis as an approach to improving or modifying a trait, or achieving basic understanding of a pathway for a trait --.
This paper provides guidelines for new high-throughput screening methods – both phenotypic and genotypic – to enable the detection of rare mutant traits, and reviews techniques for increasing the efficiency of crop mutation breeding.
This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.
In this incisive, concise overview of this booming field, the editors -- two of the leading figures in the field with a proven track record -- combine their expertise to provide an invaluable reference on the topic. Following a treatment of transcriptome analysis, the book goes on to discuss replacement and mutation analysis, gene silencing and computational analysis. The whole is rounded off with a look at emerging technologies. Each chapter is accompanied by a concise overview, helping readers to quickly identify topics of interest, while important, carefully selected words and concepts are explained in a handy glossary. Equally accessible to both experienced scientists and newcomers to the field.
Mutation detection is increasingly undertaken in a wide spectrum of research areas: in medicine it is fundamental in isolating disease genes and diagnbosis, and is especially important in cancer research; in biology, commercially important genes can be identified by the mutations they contain. But mutation detection is time-consuming and expensive. This volume offers the latest tried and tested protocols for a range of detection methods, from the labs of the leading researchers in the field.
This book highlights the recent progress on the applications of mutation breeding technology in crop plants. Plant breeders and agriculturists are faced with the new challenges of climate change, human population growth, and dwindling arable land and water resources which threaten to sustain food production worldwide. Genetic variation is the basis which plant breeders require to produce new and improved cultivars. The understanding of mutation induction and exploring its applications has paved the way for enhancing genetic variability for various plant and agronomic characters, and led to advances in gene discovery for various traits. Induced mutagenesis has played a significant role in crop improvement and currently, the technology has resulted in the development and release of more than 3600 mutant varieties in most of the crop plants with great economic impact. The field of ‘mutation breeding’ has come long way to become an important approach for crop improvement. This book covers various methodologies of mutation induction, screening of mutants, genome editing and genomics advances and mutant gene discovery. The book further discusses success stories in different countries and applications of mutation breeding in food crops, horticultural plants and plantation crops. This informative book is very useful to plant breeders, students and researchers in the field of agriculture, plant sciences, food science and genetics.
This is the newest title in the successful Molecular Plant Biology Handbook Series. Just like the other titles in the series this new book presents an excellent overview of different approaches and techniques in Metabolomics. Contributors are either from ivy-league research institutions or from companies developing new technologies in this dynamic and fast-growing field. With its approach to introduce current techniques in plant metabolomics to a wider audience and with many labs and companies considering to introduce metabolomics for their research, the title meets a growing market. The Kahl books are in addition a trusted brand for the plant science community and have always sold above expectations.
With the aim of helping to combat the challenge of providing enough food for the world, this comprehensive three-volume set book, Biotechnologies and Genetics in Plant Mutation Breeding, focuses on improvement of plant agronomic characters by manipulating the genotype of plant species with induced mutagenesis. Volume 3: Mechanisms for Genetic Manipulation of Plants covers the different genetic engineering techniques that are used to mutant genes and to incorporate them in different plant species of cereals, pulses, vegetables, and fruits. Chapters also address in-vitro mutagenesis, which allows specific mutations to be tailored for type and position within the gene. Also discussed is the role of molecular techniques and methods for mutation detection and screening in plants and the role of genetic manipulation of plants and microorganisms and the hazardous impacts on them. There is emphasis on improvement of agronomic characters by manipulating the genotype of plant species, resulting in increase in food crop productivity. Importantly, the principles of genetic engineering in which mechanism and action of transfer of desired genes from plants to animals to microorganism and vice versa are also presented. Volume 1: Mutagenesis and Crop Improvement provides important coverage of mutagenesis, cytotoxicity, and crop improvement in plants along with explanations of the processes, mutagenic effectiveness, and efficiency and mechanisms of mutagenesis. The authors also cover the principles, applications, and scope of mutagenesis. Several chapters focus on mutation-induced cytological aberrations and cytotoxicity and on in-vitro mutagenesis to exploit the somaclonal variations induced in cell culture. There is also emphasis on improvement of agronomic characteristics by manipulating the genotype of plant species, resulting in increase in productivity by the use of induced mutagenesis. Volume 2: Revolutionizing Plant Biology presents the use of mutagenesis and biotechnology to explore the variability of mutant genes for crop improvement. In this volume, the chapters deal with in-vitro mutagenesis to exploit the somaclonal variations induced in cell culture. The chapters highlight the importance of in-vitro mutagenesis in inducing salt resistance, heat resistance, and draught resistance. The chapters are supported by authentic data of the Food and Agriculture Organization of the United Nations and other international agencies that highlight the role of mutagenesis in crop improvement. With its focus on promising techniques to help to combat food scarcity in the world both in quality and quantity, these three volumes will be a valuable asset for advanced students and faculty in biotechnology and genetic engineering as well as those in botany, agriculture, and horticulture.