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A method is described for preparing membrane filters for electron microscopic observation.
Membrane filters are partially permeable layers made of cellulose esters which are employed to separate coarsely dispersed and colloidal particle sizes from substances. The electron microscope is necessary in many cases in order to make visible the particles separated by the filter. Since the membrane filter by reason of its film thickness ranging from 50 to 200 microns is impermeable to electrons, preparation problems result, and it is to the solution of these that the work concerns.
An eclectic mix of studies on chemical and electrochemical behaviour of membrane surfaces. The book looks at membranes - both organic and inorganic - from a host of different perspectives and in the context of many diverse disciplines. It explores the behaviours of both synthetic and biological membranes, employing physical, chemical and physiochemical perspectives, and blends state-of-the-art research of many disciplines into a coherent whole.
A detailed treatment of information relating to fluid-oxide interfaces. It outlines methods for quantifying adsorption and desorption of polymeric and non-polymeric solutes at the gas- and solution-oxide interfaces. It also analyzes novel properties of oxide membranes and the synthesis and dissolution of oxide solids.
A guide to modern scanning electron microscopy instrumentation, methodology and techniques, highlighting novel applications to cell and molecular biology.
The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field Includes a brief history and overview on CLEM methods