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The Chlamydomonas Sourcebook, 3rd Edition Cell Motility and Behavior (Volume 3) The gold-standard reference?introducing this multidisciplinary science,?fully revised and updated with the latest discoveries Originally published as the standalone Chlamydomonas Sourcebook, then expanded as the third volume in a three-part comprehensive gold-standard reference,?The Chlamydomonas Sourcebook: Cell Motility and Behavior has been fully revised and updated to include the wealth of new resources for the Chlamydomonas community. Reflecting the significant advancement in?the understanding of the role of basal bodies and cilia play in human diseases, this volume employs quantitative proteomics and mass spectroscopy as well as?cryo?EM tomography and single particle?cryo?EM. Other topics such as current insights?on?mitosis and cytokinesis, ciliary assembly and motility, intraflagellar?transport, and more help build an understanding of human diseases of the cilium. Cell Motility and Behavior presents the latest in research and best practices, making this a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, and biochemistry; crop scientists; plant physiologists; and plant, molecular, and human disease biologists. Provides an essential reference to a model species for the study of mechanisms of motility in free living cells? Includes methods for Chlamydomonas motility research? Includes a table listing the known proteins (with NCBI accession numbers) for each structure discussed, and the known mutations that affect each structure and process
Dr. Harris has played a major role in the development of this organism as a model system. Her previous version of the Chlamydomonas Sourcebook which published in 1989, has been a classic in the field and is considered required reading for anyone working with this organism. This latest edition has been expanded to include three volumes providing molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism. Methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media, are included. Additions to this volume also include help with common laboratory problems such as contamination, student demonstrations, and properties of particular strains and mutants. This volume is part of a 3-Volume Set (ISBN: 978-0-12-370873-1) and is also sold individually. - Expanded revision of gold standard reference - Includes latest advances in research, including completion of the genome - Provides broad perspective with studies in cell and molecular biology, genetics, plant physiology and related fields - Available as part of a 3-Volume Set or sold individually
This second volume of The Chlamydomonas Sourcebook provides the background and techniques for using this important organism in plant research. From biogenesis of chloroplasts and mitochondria and photosynthesis to respiration and nitrogen assimilation, this volume introduces scientists to the functions of the organism. The volume then moves on to starch biosynthesis, sulfur metabolism, response to heavy metals, and hydrogen production. - Describes molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism - Includes contributions from leaders in particular areas of research - Provides methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media - Assists researchers with common laboratory problems such as contamination - Includes valuable student demonstrations and properties of particular strains and mutants - Edited by the leading researcher in Chlamydomonas science
This volume reviews virtually everything that is known about cell motility and behavior in Chlamydomonas. World experts in each area focus on mitosis and cytokinesis; flagellar assembly and motility; intraflagellar transport, and more will use this reference as a guide for understanding human diseases of the cilium. The volume is richly illustrated and is supplemented by a Website containing both classic and previously unpublished videos of cell motility in Chlamydomonas. Because Chlamydomonas has been the premier model for investigating the function and behavior of cilia and flagella, the chapters summarize the current state of knowledge in these areas as it applies to all ciliated organisms. Thus, this volume will be an essential source for all students and researchers interested in cell motility. This volume is part of a 3-Volume Set (ISBN: 978-0-12-370873-1) and is also sold individually. - Includes a table listing the known proteins (with NCBI accession numbers) for each structure discussed, and the known mutations that affect each structure and process - Essential reference to a model species for the study of mechanisms of motility in free living cells - Includes methods for Chlamydomonas motility research - Companion Website with videos illustrates the marvels of the Chlamydomonas flagella in action
Originally published as the stand-alone Chlamydomonas Sourcebook, then expanded as the second volume in a three-part comprehensive gold-standard reference, The Chlamydomonas Sourcebook: Organellar and Metabolic Processes has been fully revised and updated to include a wealth of new knowledge and resources for the Chlamydomonas community. It details the tremendous progress recently made with respect to imaging the ultrastructure of cells, dissecting acclimation and biosynthetic responses, and elucidating molecular processes underlying the biology of organelles. In particular, this volume includes exciting new developments in the use of imaging technologies for examining supramolecular organization of the chloroplast, defining mechanisms of branched electron transfer pathways in photosynthesis, dissecting the organization of pyrenoids and CO2 concentration mechanisms, presenting the intricacies associated with acclimation to environmental conditions and providing new insights into dark metabolism and the network of fermentative metabolism. This book thus presents the latest advances in both the research and uses of new experimental approaches and technologies, making this a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, aspects of human disease, acclimation to environmental change, and the biogenesis of cellular complexes. - Describes molecular techniques, analysis of the recently sequenced genome, reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism - Provides methods for Chlamydomonas research and best practices for their applications; this includes methods for cell culture, preservation of cultures, preparation of media, lists of inhibitors, and other additives to culture media, classical genetic manipulation, and new approaches for gene transfer and editing technologies - Assists researchers with common laboratory problems such as contamination
The Chlamydomonas Sourcebook, 3rd Edition Introduction to Chlamydomonas and Its Laboratory Use (Volume 1) The gold-standard reference?covering the basic biology of the Chlamydomonas alga and techniques for its laboratory analysis Originally published as the standalone Chlamydomonas Sourcebook, then expanded as the first volume in a three-part comprehensive gold-standard reference,?The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use?has been fully revised and updated to include a?wealth of new resources for the Chlamydomonas community. Early chapters cover current understandings of its taxonomy, ultrastructure, cell and life cycles, and nuclear and organelle genomes, followed by technique-oriented chapters covering such topics as cell culture, mutagenesis, genetic analysis, construction of mutant libraries, and protein localization using immunofluorescence. This volume presents the latest in research and best practices, making it a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, and biochemistry; crop scientists; plant physiologists; and plant, molecular, and human disease biologists. - Remains the only complete reference to provide both the historical background and the most up-to-date information and applications on Chlamydomonas - Includes best practices for applications in research, including methods for culture, genetic analysis, genomic and transcriptomic analysis, and mutant screening - Helps researchers solve common laboratory problems, provides details on the properties of particular strains, and offers a comprehensive survey of molecular approaches - Provides a broad perspective for studies in cell and molecular biology, genetics, plant physiology, and related fields
The green alga Chlamydomonas is widely used as an experimental model system for studies in cellular and molecular biology, and in particular plant molecular biology. This book is the only single modern compendium of information on its biology and in particular its molecular biology and genetics. Included in addition to much information on the basic biology is material of a very practical nature, namely, methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media, help with common laboratory problems such as contamination, student demonstrations, and properties of particular strains and mutants. Casual users as well as specialists will find the book to be useful in many ways. - Provides access to previously unpublished data from genetic analysis - Provides descriptions of mutant strains - Depicts summary tables comparing properties of different species and their mutant strains - Explains detailed methods for laboratory procedures of general utility - Furnishes comparisons of culture media - Presents lists of inhibitors, mutagens, and other additives to culture media - Assists with common laboratory problems such as contamination and storage of strains - Demonstrates protocols for laboratory demonstrations available for undergraduate teaching.
Along with its companion volume on intraflagellar transport, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella. - Covers protocols for cilia and flagella across systems and species - Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time - Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures
The biological sciences cover a broad array of literature types, from younger fields like molecular biology with its reliance on recent journal articles, genomic databases, and protocol manuals to classic fields such as taxonomy with its scattered literature found in monographs and journals from the past three centuries. Using the Biological Litera
Cell Polarity in Development and Disease, Volume 154 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including Cell polarity in the protist-to-animal transition, Polarized actin networks in development: Case studies from Drosophila, Protein clustering and cell polarization, Polarity in the Drosophila female germline, Context Matters: Mechanisms governing epithelial polarization in C. elegans, Epithelial polarity in the fly: Principles and diversity, Polarizing epithelial expulsion and expulsion driven by mispolarization, The Role of Apical-Basal Polarization in the Mammalian First Cell Fate Decision, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Cell Biology series - Updated release includes the latest information on Cell Polarity in Development and Disease