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Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions and define their components, their manner of interaction, and their manner of function, both in the cell and in the test tube. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to understand DNA-protein interactions.
Microbial Gene Techniques is a practical laboratory guide to current techniques of molecular biology and genetics. The focus of the volume is on microbial cells, particularly eukaryotic microbes and bacteria, as well as plasmids and bacteriophages.* * Methods presented for ease of use and ready adaptation to new systems.* Detailed protocols included for:* Eukaryotic microbes - protozoan parasites (forward and reverse genetics, genome analysis), filamentous fungi (chromosome and gene analysis)* Yeast chromosomes - YACs, genome mapping, transcription factors, nucleosomes, recombination, RNA polymerase, pheromones.* Bacterial gene structure and regulation - E. coli (DNA methylation, mRNA characterization, gene regulation), B Subtilis (genetic mapping, chemotaxis), computer identification of genes.* Plasmids and bacteriophages - plasmid templates for transcription assays, plasmid replication: bacteriophage transcription, molecular genetic analysis using phages, phage assembly.
Genetic Analysis applies the combined power of molecular biology, genetics, and genomics to explore how the principles of genetics can be used as analytical tools to solve biological problems. This new edition: Illustrates the conceptual basis of key analytical tools with carefully selected examples from a range of model organisms, and encourages the reader to Look beyond the examples to see how these tools can be used to explore a wide range of biological questions, Covers the latest and most powerful experimental tools to provide a state-of-the-art review of the field, giving insights into gene networks and interactions, Includes extended case studies that enable the reader to fully get to grips with how genetic tools can be used to understand biological systems in the real world. New to This Edition: A new chapter on genome editing with focus on the CRISPR-Cas 9 system, New content on the analysis of gene activity using temperature-sensitive mutations and mosaics, Increased coverage of epigenetics, updated with the latest developments in the field, A new Learning feature called "Literature Link," which connects each chapter's content to cutting-edge research. The online resources to accompany Genetic Analysis feature the following material for students and teachers: For students: Practice problems and solutions to test your knowledge of the concepts presented, and help you to master them, Online datasets with which to practise analytic techniques, For registered adopters of the book: Figures from the book in electronic format, ready to download, Journal clubs-suggested papers and discussion questions linked to topics covered in the book. Book jacket.
A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.